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Nonmammalian Genomic Analysis A Practical Guide / Edited by Bruice Birren and Eric Lai.
Lai, Eric Hon Cheong (författare)
Birren, Bruce W. (redaktör/utgivare)
Lai, Eric Hon Cheong (redaktör/utgivare)
- San Diego : Academic Press, 1996
- Engelska online resource (xiv, 353 s.
- Relaterad länk:
http://www.sciencedi... (Table of contents)
- 1. Introduction to Pulsed-Field Gels and Preparation and Analysis of Large DNA -- 2. Electrophoretic Karyotyping in Fungi -- 3. Isolation and Analysis of High-Molecular-Weight DNA from Plants -- 4. Generating and Using DNA Markers in Plants -- 5. Genome Mapping of Protozoan Parasites by Linking Clones -- 6. Macrorestriction Mapping and Analysis of Bacterial Genomes -- 7. Cosmid Cloning with Small Genomes -- 8. Construction of P1 Artificial Chromosome (PAC) Libraries from Lower Vertebrates -- 9. The Selection of Chromosome-Specific DNA Clones from African Trypanosome Genomic Libraries -- 10. Analysis of the Dictyostelium discoideum Genome -- 11. Integrated Genome Mapping by Hybridization Techniques.
- Offering detailed protocols for those needing to construct a variety of maps and isolate genes, this unique book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. Nonmammalian Genomic Analysis: A Practical Guide covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning. Like having a local expert, this collection provides procedures for anyone, anywhere, and allows the replication of others' success.