Quantitative Proteomics of Yeast Post-Golgi Vesicles Reveals a Discriminating Role for Sro7p in Protein Secretion

• We here report the first comparative proteomics of purified yeast post-Golgi vesicles (PGVs). Vesicle samples isolated from PGV-accumulating sec6-4 mutants were treated with isobaric tags (iTRAQ) for subsequent quantitative tandem mass spectrometric analysis of protein content. After background subtraction, a total of 66 vesicle-associated proteins were identified, including known or assumed vesicle residents as well as a fraction not previously known to be PGV associated. Vesicles isolated from cells lacking the polarity protein Sro7p contained essentially the same catalogue of proteins but showed a reduced content of a subset of cargo proteins, in agreement with a previously shown selective role for Sro7p in cargo sorting.

Ämnesord

NATURVETENSKAP  -- Biologi -- Cellbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Cell Biology (hsv//eng)

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

Exocytosis

Golgi

ITRAQ

Membrane trafficking

Proteomics

Vesicles

cell protein

protein sro7p

unclassified drug

article

cell vacuole

Golgi complex

nonhuman

priority journal

protein analysis

protein content

protein secretion

protein targeting

quantitative analysis

tandem mass spectrometry

yeast

Adaptor Proteins

Signal Transducing

Biological Markers

Cytoplasmic Vesicles

Golgi Apparatus

Protein Transport

Recombinant Fusion Proteins

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Adaptor Proteins

Signal Transducing

genetics

metabolism

Biological Markers

Cytoplasmic Vesicles

chemistry

Golgi Apparatus

Protein Transport

Proteomics

methods

Recombinant Fusion Proteins

Saccharomyces cerevisiae

cytology

Saccharomyces cerevisiae Proteins

classification

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)