The protein expression of TRP-1 and galectin-1 in cutaneous malignant melanomas

• Background: Patients with metastazing malignant melanoma have a poor outcome and determination of thickness of the primary tumor remains as the most important prognostic predictor. The aim of this study was to use an antibody-based proteomics strategy to search for new molecular markers associated with melanoma progression. Two proteins, TRP-1 and galectin-1, were identified as proteins with enhanced expression in cells from the melanocytic lineage. Patients and Methods: Protein profiling of TRP-1 and galectin-1 together with proliferation marker Ki-67 and melanocyte marker Melan-A was performed in normal tissues from 144 individuals and in 216 different tumors using tissue microarrays and immunohistochemistry. The protein expression pattern was further analyzed in a defined cohort of 157 patients diagnosed with invasive cutaneous malignant melanoma. Results: Both TRP-1 and galectin-1 were highly expressed in normal melanocytes and melanoma. The expression of TRP-1 was inversely correlated with tumor stage (p=0.002, (R=-0.28)). Neither TRP-1 or galectin-1 was associated with overall or disease free survival (p>0.14, p>0.46 respectively). Ki-67 was associated with tumor stage and survival (p<0.001). Conclusion: TRP-1 and galectin-1 protein expression patterns were determined in normal and cancer tissues and both proteins were expressed in the majority of the malignant melanomas. There was no correlation between TRP-1 or galectin-1 expression and survival.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology (hsv//eng)

Nyckelord

Galectin-1

Human Protein Atlas Program

Malignant melanoma

TRP-1

galectin 1

glycoprotein

Ki 67 antigen

melan A

molecular marker

protein antibody

protein TRP 1

unclassified drug

article

cancer growth

cancer staging

cancer tissue

controlled study

disease free survival

female

human

immunohistochemistry

major clinical study

male

melanocyte

melanoma

overall survival

protein analysis

protein expression

proteomics

skin carcinogenesis

tissue microarray

Disease-Free Survival

Follow-Up Studies

Gene Expression Profiling

Gene Expression Regulation

Neoplastic

Humans

Ki-67 Antigen

Melanocytes

Membrane Glycoproteins

Neoplasm Invasiveness

Neoplasm Metastasis

Neoplasm Proteins

Oxidoreductases

Retrospective Studies

Skin Neoplasms

Survival Rate

MEDICINE

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