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Cellulose synthesis...
Cellulose synthesis in Phytophthora infestans is required for normal appressorium formation and successful infection of potato
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- Grenville-Briggs, Laura J. (author)
- Aberdeen Oomycete Group, University of Aberdeen, Institute of Medical Sciences
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- Anderson, Victoria L. (author)
- Aberdeen Oomycete Group, University of Aberdeen, Institute of Medical Sciences
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- Fugelstad, Johanna (author)
- KTH,Glykovetenskap
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- Avrova, Anna O. (author)
- Plant-Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee
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- Bouzenzana, Jamel (author)
- Organisation et Dynamique des Membranes Biologiques, Unité Mixte de Recherche 5246, Université Lyon I
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- Williams, Alison (author)
- Aberdeen Oomycete Group, University of Aberdeen, Institute of Medical Sciences
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- Wawra, Stephan (author)
- Aberdeen Oomycete Group, University of Aberdeen, Institute of Medical Sciences
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- Whisson, Stephen C. (author)
- Plant-Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee
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- Birch, Paul R. J. (author)
- Plant-Pathology Programme, Scottish Crop Research Institute, Invergowrie, Dundee
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- Bulone, Vincent (author)
- KTH,Glykovetenskap
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- van West, Pieter (author)
- Aberdeen Oomycete Group, University of Aberdeen, Institute of Medical Sciences
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(creator_code:org_t)
- 2008-03-18
- 2008
- English.
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In: The Plant Cell. - : Oxford University Press (OUP). - 1040-4651 .- 1532-298X. ; 20:3, s. 720-738
- Related links:
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http://www.plantcell...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Cellulose, the important structural compound of cell walls, provides strength and rigidity to cells of numerous organisms. Here, we functionally characterize four cellulose synthase genes (CesA) in the oomycete plant pathogen Phytophthora infestans, the causal agent of potato (Solanum tuberosum) late blight. Three members of this new protein family contain Pleckstrin homology domains and form a distinct phylogenetic group most closely related to the cellulose synthases of cyanobacteria. Expression of all four genes is coordinately upregulated during pre- and early infection stages of potato. Inhibition of cellulose synthesis by 2,6-dichlorobenzonitrile leads to a dramatic reduction in the number of normal germ tubes with appressoria, severe disruption of the cell wall in the preinfection structures, and a complete loss of pathogenicity. Silencing of the entire gene family in P. infestans with RNA interference leads to a similar disruption of the cell wall surrounding appressoria and an inability to form typical functional appressoria. In addition, the cellulose content of the cell walls of the silenced lines is >50% lower than in the walls of the nonsilenced lines. Our data demonstrate that the isolated genes are involved in cellulose biosynthesis and that cellulose synthesis is essential for infection by P. infestans.
Subject headings
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Keyword
- fungus saprolegnia-monoica
- v chitin synthase
- cell-walls
- sequence
- alignment
- plant interactions
- in-vitro
- biosynthesis
- oomycete
- gene
- enzymes
- Bioengineering
- Bioteknik
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Grenville-Briggs ...
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Anderson, Victor ...
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Fugelstad, Johan ...
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Avrova, Anna O.
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Bouzenzana, Jame ...
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Williams, Alison
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show more...
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Wawra, Stephan
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Whisson, Stephen ...
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Birch, Paul R. J ...
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Bulone, Vincent
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van West, Pieter
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show less...
- About the subject
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
- Articles in the publication
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The Plant Cell
- By the university
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Royal Institute of Technology