Tyck till om SwePub Sök
här!
Sökning: onr:"swepub:oai:DiVA.org:uu-270621" >
Removal of xenotrop...
Removal of xenotropic murine leukemia virus by nanocellulose based filter paper
-
- Asper, M. (författare)
- Charles River Biopharmaceut Serv GmbH, D-51105 Cologne, Germany.,Charles River Biopharmaceutical Services GmbH, Gottfried-Hagen Str. 20, 51105, Köln, Germany
-
- Hanrieder, T. (författare)
- Charles River Biopharmaceut Serv GmbH, D-51105 Cologne, Germany.,Charles River Biopharmaceutical Services GmbH, Gottfried-Hagen Str. 20, 51105, Köln, Germany
-
- Quellmalz, Arne (författare)
- Uppsala universitet,Nanoteknologi och funktionella material,Nanotechnology and Functional Materials, Department of Engineering Sciences, Box 534, Uppsala University, 75121 Uppsala, Sweden
-
visa fler...
-
- Mihranyan, Albert (författare)
- Uppsala universitet,Nanoteknologi och funktionella material,Nanotechnology and Functional Materials, Department of Engineering Sciences, Box 534, Uppsala University, 75121 Uppsala, Sweden
-
visa färre...
-
Charles River Biopharmaceut Serv GmbH, D-51105 Cologne, Germany Charles River Biopharmaceutical Services GmbH, Gottfried-Hagen Str. 20, 51105, Köln, Germany (creator_code:org_t)
- Elsevier BV, 2015
- 2015
- Engelska.
-
Ingår i: Biologicals (Print). - : Elsevier BV. - 1045-1056 .- 1095-8320. ; 43:6, s. 452-456
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- The removal of xenotrpic murine leukemia virus (xMuLV) by size-exclusion filter paper composed of 100% naturally derived cellulose was validated. The filter paper was produced using cellulose nanofibers derived from Cladophora sp. algae. The filter paper was characterized using atomic force microscopy, scanning electron microscopy, helium pycnometry, and model tracer (100 nm latex beads and 50 nm gold nanoparticles) retention tests. Following the filtration of xMuLV spiked solutions, LRV >= 5.25 log(10) TCID50 was observed, as limited by the virus titre in the feed solution and sensitivity of the tissue infectivity test. The results of the validation study suggest that the nanocellulose filter paper is useful for removal of endogenous rodent retroviruses and retrovirus-like particles during the production of recombinant proteins.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Viral contamination
- Virus retentive filtration
- Cladophora cellulose
- Paper making
- Recombinant proteins and monoclonal antibodies
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas