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Significance of mRNA and Protein Expression of MAC30 in Progression of Colorectal Cancer

Zhao, Zeng-Ren, (författare)
Hebei Med Univ, Shijiazhuang, Peoples R China
Zhang, Li-Jing, (författare)
Hebei Med Univ, Shijiazhuang, Peoples R China
He, Xin-Qi, (författare)
Hebei Med Univ, Shijiazhuang, Peoples R China
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Zhang, Zhi-Yong, (författare)
Hebei Med Univ, Shijiazhuang, Peoples R China
Zhang, Feng, (författare)
Hebei Med Univ, Shijiazhuang, Peoples R China
Li, Fang, (författare)
Hebei Med Univ, Shijiazhuang, Peoples R China
Pei, Yong-Bin, (författare)
Hebei Med Univ, Shijiazhuang, Peoples R China
Hu, Yue-Ming, (författare)
Hebei Med Univ, Shijiazhuang, Peoples R China
Wang, Ming-Wei, (författare)
Hebei Med Univ, Shijiazhuang, Peoples R China
Sun, Xiao-Feng, (författare)
Linköpings universitet, Onkologi, Linköpings universitet, Hälsouniversitetet, Östergötlands Läns Landsting, Onkologiska kliniken US
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Linköpings universitet Institutionen för klinisk och experimentell medicin. Onkologi. (creator_code:org_t)
Linköpings universitet Hälsouniversitetet. (creator_code:org_t)
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Östergötlands Läns Landsting Centrum för kirurgi, ortopedi och cancervård. Onkologiska kliniken US. (creator_code:org_t)
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2011
Engelska.
Ingår i: Chemotherapy. - Basel : Karger AG. - 0009-3157. ; 57:5, s. 394-401
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • Background: Meningioma-associated protein (MAC30), first described to be overexpressed in meningiomas, exhibits altered expression in certain human tumors. The aim of our study was to investigate the expression of MAC30 mRNA and its correlation with clinicopathological variables in human colorectal cancer (CRC). Methods: MAC30 mRNA expression was first examined in 55 CRCs, along with the samples from the matched distant normal and adjacent noncancerous tissue by RT-PCR, further verified in 18 CRCs by quantitative RT-PCR. MAC30 protein expression was detected by Western blot in 10 CRCs, and DNA sequencing was performed in 1 case of the paired CRC and the matched noncancerous specimen. MAC30 mRNA expression in two colon cancer cell lines, HCT-116(p53-/-) and HCT-116(p53+/+), was detected by quantitative RT-PCR. Results: The mRNA expression of MAC30 was increased in CRC when compared with distant normal (p < 0.01) and adjacent noncancerous mucosa (p < 0.01). The mean value of MAC30 mRNA expression in the tumor located in the colon was higher than in the rectum (0.677 +/- 0.419 vs. 0.412 +/- 0.162, p = 0.005). As the tumor penetrated the wall of the colon/rectum, MAC30 mRNA expression notably increased in tumors with T3+T4 stage compared to tumors with T1+T2 stage (0.571 +/- 0.364 vs. 0.404 +/- 0.115, p = 0.014). MAC30 protein expression in CRCs was also remarkably elevated compared to the adjacent noncancerous mucosa. There was no mutation in the coding region of the MAC30 gene either in CRC or in the noncancerous mucosa. mRNA expression of p53 was notably decreased in HCT-116(p53-/-) compared to HCT-116(p53+/+), while MAC30 did not vary greatly. Conclusion: The overexpression of MAC30 might be involved in the development and aggressiveness of CRCs, especially in the colon.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  (hsv//swe)
MEDICAL AND HEALTH SCIENCES  (hsv//eng)

Nyckelord

MAC30
Colorectal cancer
HCT-116 cells
RT-PCR
Western blot
DNA sequencing
MEDICINE
MEDICIN

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