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Monitoring and quan...
Monitoring and quantification of inclusion body formation in Escherichia coli by multi-parameter flow cytometry
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- Wållberg, Fredrik (författare)
- KTH,Skolan för bioteknologi (BIO),Royal Institute of Technology, Sweden
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- Sundström, Heléne (författare)
- KTH,Skolan för bioteknologi (BIO),Royal Institute of Technology, Sweden
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- Ledung, Erika (författare)
- Mälardalens högskola,Institutionen för biologi och kemiteknik,Department of Biology and Chemical Engineering, Mälardalen University
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- Hewitt, Christopher J (författare)
- Centre for Formulation Engineering, School of Engineering (Chemical Engineering), University of Birmingham
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- Enfors, Sven-Olof (författare)
- KTH,Skolan för bioteknologi (BIO),Royal Institute of Technology, Sweden
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(creator_code:org_t)
- Springer Science and Business Media LLC, 2005
- 2005
- Engelska.
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Ingår i: Biotechnology Letters. - : Springer Science and Business Media LLC. - 0141-5492 .- 1573-6776. ; 27:13, s. 919-926
- Relaterad länk:
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http://www.springerl...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Multi-parameter flow cytometry was used to monitor the formation of promegapoietin (PMP) inclusion bodies during a high cell density Escherichia coli fed-batch fermentation process. Inclusion bodies were labelled with a primary antibody and then with a secondary fluorescent antibody. Using this method it was possible to detect PMP inclusion body formation with a high specificity and it was possible to monitor the increased accumulation of the protein with process time (6-48 mg PMP/g CDW) whilst highlighting population heterogeneity.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
Nyckelord
- Bioreactors/microbiology
- Chromatography; High Pressure Liquid
- Escherichia coli/growth & development/*metabolism
- Fermentation
- Flow Cytometry/*methods
- Fluorescent Dyes/chemistry
- Humans
- Inclusion Bodies/chemistry/*metabolism
- Interleukin-3
- Microscopy; Fluorescence
- Receptors; Interleukin-3/chemistry/genetics/metabolism
- Recombinant Fusion Proteins
- Recombinant Proteins/metabolism
- Thrombopoietin/chemistry/genetics/metabolism
- Biochemical process engineering
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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