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Sökning: onr:"swepub:oai:DiVA.org:oru-2603" > Cell adhesion prote...

Cell adhesion proteins in different invasive patterns of colon carcinomas : a morphometric and molecular genetic study

Hahn-Strömberg, Victoria, 1971- (författare)
Örebro universitet,Hälsoakademin
Franzén, Lennart (preses)
Palmqvist, Richard, Docent (opponent)
Institutionen för medicinsk biovetenskap, Umeå universitet
 (creator_code:org_t)
ISBN 9789176686409
Örebro : Örebro universitet, 2008
Engelska 61s.
Serie: Örebro Studies in Medicine, 1652-4063 ; 24
  • Doktorsavhandling (övrigt vetenskapligt)
Abstract Ämnesord
Stäng  
  • Colorectal carcinoma is the second most common type of cancer in both men and women in Sweden. Cancer of the colon and rectum are often considered together and their ten year survival rate is approximately 50 – 60 % depending on sex and location. Different histopathological characteristics of such cancers, including the complexity of growth, are of importance for prognosis. This thesis has compared different morphometric methods in order to achieve a quantitative and objective measurement of the invasive front of colon carcinoma. Since the growth pattern is dependent on the cell adhesiveness of different proteins we studied the distribution and localization of E-cadherin, Beta-catenin, Claudin 1,2,7 and Occludin as well as screened the genes for mutations. We found a perturbed protein expression of E-cadherin, Beta-catenin, Claudin 1,2,7 and Occludin in tumor sections compared to normal mucosa, but no relation to tumor volume or growth pattern could be seen. The tumor volume was found to be correlated to the growth pattern but not responsible to the perturbed protein expression. In the mutation screening we found a SNP in exon 13 the E-cadherin gene in the tumor, as well as in exon 2 of Claudin 1 and exon 4 of Claudin 7 in both tumor and normal mucosa. No correlation between mutations and growth pattern or tumor volume was found. In conclusion, this thesis shows that the computer image analysis with estimation of fractal dimension and number of free tumor cell clusters is superior to the semi quantitative visual grading of tumor invasive complexity. The aberrant expression of cell adhesion proteins in the tumor compared to normal mucosa as well as polymorphisms in the cell adhesion genes CLDN1 and CLDN7 in both tumor and normal mucosa can suggest that these aberrations are important in the tumorigenesis of colon carcinoma.  

Ämnesord

AGRICULTURAL SCIENCES  -- Veterinary Science -- Clinical Science (hsv//eng)
LANTBRUKSVETENSKAPER  -- Veterinärmedicin -- Klinisk vetenskap (hsv//swe)
LANTBRUKSVETENSKAPER  -- Veterinärmedicin (hsv//swe)
AGRICULTURAL SCIENCES  -- Veterinary Science (hsv//eng)

Nyckelord

colon carcinoma
growth pattern
tight junction
Complexity Index
cell adhesion
E-cadherin
Beta-catenin
Occludin
Claudin.
Medicine
Medicin
Biomedicin
Biomedicine

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