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Sökning: onr:"swepub:oai:DiVA.org:oru-34517" > Inhibition of 12-li...

Inhibition of 12-lipoxygenase reduces platelet activation and prevents their mitogenic function

Holm, Ann-Charlotte B. Svensson (författare)
Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Department of Medical and Health Sciences, Faculty of Health Sciences, Linköping University, Linköping, Sweden
Grenegård, Magnus, 1963- (författare)
Linköpings universitet,Örebro universitet,Institutionen för läkarutbildning,Department of Medical and Health Sciences, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Department of Clinical Pathology and Clinical Genetics, County Council of Östergötland, Linköping, Sweden,Avdelningen för läkemedelsforskning,Hälsouniversitetet,Örebro University, Sweden
Ollinger, Karin (författare)
Östergötlands Läns Landsting,Linköpings universitet,Avdelningen för cellbiologi,Hälsouniversitetet,Klinisk patologi och klinisk genetik,Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden; Department of Clinical Pathology and Clinical Genetics, County Council of Östergötland, Linköping, Sweden
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Lindström, Eva G. (författare)
Department of Medical and Health Sciences, Faculty of Health Sciences, Linköping University, Linköping, Sweden
Lindström, Eva (författare)
Linköpings universitet,Avdelningen för läkemedelsforskning,Hälsouniversitetet
Svensson Holm, Ann-Charlotte (författare)
Linköpings universitet,Avdelningen för cellbiologi,Avdelningen för läkemedelsforskning,Hälsouniversitetet
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 (creator_code:org_t)
London, United Kingdom : Informa Healthcare, 2014
2014
Engelska.
Ingår i: Platelets. - London, United Kingdom : Informa Healthcare. - 0953-7104 .- 1369-1635. ; 25:2, s. 111-117
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • The aim of the present study was to investigate the role of 12-lipoxygenase (12-LOX) on platelet-induced airway smooth muscle cell (ASMC) proliferation. Co-incubation of platelets and ASMC caused platelet activation as determined by morphological changes. Simultaneously, reactive oxygen species (ROS)-generation was detected and ASMC proliferation (measured by using the MTS assay) increased significantly. Furthermore, we found that the 12-LOX inhibitors cinnamyl-3,4-dihydroxy-a-cyanocinnamate (CDC) and Baicalein prevented platelet activation in a co-cultures of platelets and ASMC. The inhibitory effect of CDC and Baicalein on platelets was also registered in a pure platelet preparation. Specifically, the 12-LOX inhibitors reduced collagen-induced platelet aggregation both in the presence and absence of external added fibrinogen. Importantly, platelet-induced ASMC proliferation and ROS production generated during the platelet/ASMC interaction was significantly inhibited in the presence of 12-LOX inhibitors. In conclusion, our findings reveal that 12-LOX is crucial for the observed enhancement of ASMC proliferation in co-cultures of platelets and ASMC. The present result suggests that 12-LOX activity is important in the initial step of platelet/ASMC interaction and platelet activation. Such action of 12-LOX represents a potential important mechanism that may contribute to platelet-induced airway remodelling.

Ämnesord

NATURAL SCIENCES  -- Biological Sciences -- Cell Biology (hsv//eng)
NATURVETENSKAP  -- Biologiska vetenskaper -- Cellbiologi (hsv//swe)
NATURVETENSKAP  -- Biologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

12-lipoxygenase
airway remodelling
airway smooth muscle
platelet-induced proliferation
Cellforskning
Cell Research
Medicine
Medicin

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