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Molecular analysis of the gut microbiota of identical twins with Crohn's disease

Dicksved, Johan (författare)
Halfvarson, Jonas (författare)
Rosenquist, Magnus (författare)
Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
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Järnerot, Gunnar (författare)
Tysk, Curt (författare)
Örebro universitet,Hälsoakademin
Apajalahti, Juha (författare)
Engstrand, Lars (författare)
Jansson, Janet K. (författare)
Jansson, Janet K (författare)
Apajalahti, J (författare)
Tysk, C (författare)
Jarnerot, G (författare)
Halfvarson, J (författare)
Engstrand, L (författare)
Karolinska Institutet
Rosenquist, M (författare)
Jansson, JK (författare)
Dicksved, J (författare)
visa färre...
 (creator_code:org_t)
2008
2008
Engelska.
Ingår i: The ISME journal. - 1751-7370. ; 2:7, s. 716-727
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Increasing evidence suggests that a combination of host genetics and the composition of the gut microbiota are important for development of Crohn's disease (CD). Our aim was to study identical twins with CD to determine microbial factors independent of host genetics. Fecal samples were studied from 10 monozygotic twin pairs with CD (discordant n=6 and concordant n=4) and 8 healthy twin pairs. DNA was extracted, 16S rRNA genes were PCR amplified and T-RFLP fingerprints generated using general bacterial and Bacteroides group-specific primers. The microbial communities were also profiled based on their percentage G+C contents. Bacteroides 16S rRNA genes were cloned and sequenced from a subset of the samples. The bacterial diversity in each sample and similarity indices between samples were estimated based on the T-RFLP data using a combination of statistical approaches. Healthy individuals had a significantly higher bacterial diversity compared to individuals with CD. The fecal microbial communities were more similar between healthy twins than between twins with CD, especially when these were discordant for the disease. The microbial community profiles of individuals with ileal CD were significantly different from healthy individuals and those with colonic CD. Also, CD individuals had a lower relative abundance of B. uniformis and higher relative abundances of B. ovatus and B. vulgatus. Our results suggest that genetics and/or environmental exposure during childhood, in part, determine the gut microbial composition. However, CD is associated with dramatic changes in the gut microbiota and this was particularly evident for individuals with ileal CD.

Ämnesord

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Gastroenterologi (hsv//swe)

Nyckelord

Adolescent
Adult
Aged
Bacteroides/classification/genetics/*isolation & purification
Base Composition
Biodiversity
Cohort Studies
Crohn Disease/*microbiology
DNA; Bacterial/genetics
DNA; Ribosomal/genetics
Diseases in Twins/*microbiology
Feces/microbiology
Female
Gastrointestinal Tract/*microbiology
Humans
Male
Middle Aged
Multivariate Analysis
Polymorphism; Restriction Fragment Length
RNA; Ribosomal; 16S/genetics
Sweden
MEDICINE
MEDICIN
Internal medicine
Invärtesmedicin
Gastroenterology
Gastroenterologi
Internal Medicine
Invärtesmedicin

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