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Sökning: onr:"swepub:oai:DiVA.org:oru-49518" > Expression of topoi...

Expression of topoisomerase IIalpha in the G0/G1 cell cycle phase of fresh leukemic cells

Uggla, Bertil, 1962- (författare)
Department of Medicine, Örebro Medical Center Hospital, Örebro, Sweden
Möllgård, Lars, (författare)
Department of Hematology, Huddinge Uniersity Hospital, Huddinge, Sweden
Ståhl, Elisabeth, (författare)
Department of Pathology, Örebro Medical Center Hospital, Örebro, Sweden
visa fler...
Mossberg, Lise-Lott Mossberg, (författare)
Department of Pathology, Örebro Medical Center Hospital, Örebro, Sweden
Karlsson, Mats G., 1960- (författare)
Department of Pathology, Örebro Medical Center Hospital, Örebro, Sweden
Paul, Christer, (författare)
Department of Pathology, Örebro Medical Center Hospital, Örebro, Sweden; Karolinska Institute, Stockholm, Sweden
Tidefelt, Ulf, 1951- (författare)
Department of Medicine, Örebro Medical Center Hospital, Örebro, Sweden; Karolinska Institute, Stockholm, Sweden
Uggla, B (författare)
Mollgard, L, (författare)
Karolinska Institutet
Stahl, E (författare)
Mossberg, LL (författare)
Karlsson, MG (författare)
Paul, C, (författare)
Karolinska Institutet
Tidefelt, U (författare)
visa färre...
2001
Engelska.
Ingår i: Leukemia research : a Forum for Studies on Leukemia and Normal Hemopoiesis. - Oxford, United Kingdom : Elsevier. - 0145-2126. ; 25:11, s. 961-966
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • Topoisomerase IIalpha (topoII alpha) is the target enzyme for several antineoplastic drugs. Correlation between low expression of topo IIalpha and drug resistance has been shown in vitro, but there is limited evidence of a correlation to initial response to treatment or to overall prognosis. Normal cells express topo IIalpha in S/G2/M phase of the cell cycle but not in G0/G1 phase. However, some data suggest that topo IIalpha could be expressed in G0/G1 phase in malignant cells. We have investigated the expression of topo IIalpha in leukemic cells from 25 patients with acute leukemia by flow cytometry, separating cells of different cell cycle phases. We demonstrated that 9/25 samples showed >50% positive cells in G0/G1, and another five samples showed >20%. This finding could possibly provide an explanation to previous difficulties in correlating topo IIalpha expression with clinical outcome. Six of eight patients, where >20% of the cells in G0/G1 were positive for topo IIalpha, entered CR, compared to one of five patients with <20% topo IIalpha positive cells in G0/G1. We suggest that topo IIalpha expression in G0/G1 in leukemic cells may be of predictive value for clinical response to cytostatic drugs.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)

Nyckelord

Acute leukemia
topoisomerase IIalpha
drug resistance
flow cytometry
cell cycle

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