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Sökning: onr:"swepub:oai:DiVA.org:su-93766" > Immunogenic and Ant...

Immunogenic and Antioxidant Effects of a Pathogen-Associated Prenyl Pyrophosphate in Anopheles gambiae

Lindberg, Bo G (författare)
Merritt, Eleanor A (författare)
Rayl, Melanie (författare)
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Liu, Chenxiao, (författare)
Uppsala universitet, Institutionen för medicinsk cellbiologi
Parmryd, Ingela, (författare)
Uppsala universitet, Institutionen för medicinsk cellbiologi
Olofsson, Berit (författare)
Faye, Ingrid (författare)
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Uppsala universitet Medicinska och farmaceutiska vetenskapsområdet. Medicinska fakulteten. Institutionen för medicinsk cellbiologi. (creator_code:org_t)
Stockholms universitet Naturvetenskapliga fakulteten. Institutionen för molekylär biovetenskap, Wenner-Grens institut. (creator_code:org_t)
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Stockholms universitet Naturvetenskapliga fakulteten. Institutionen för organisk kemi. (creator_code:org_t)
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2013
Engelska.
Ingår i: PLoS ONE. - 1932-6203. ; 8:8, s. e73868
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • Despite efficient vector transmission, Plasmodium parasites suffer great bottlenecks during their developmental stages within Anopheles mosquitoes. The outcome depends on a complex three-way interaction between host, parasite and gut bacteria. Although considerable progress has been made recently in deciphering Anopheles effector responses, little is currently known regarding the underlying microbial immune elicitors. An interesting candidate in this sense is the pathogen-derived prenyl pyrophosphate and designated phosphoantigen (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), found in Plasmodium and most eubacteria but not in higher eukaryotes. HMBPP is the most potent stimulant known of human V gamma 9V delta 2 T cells, a unique lymphocyte subset that expands during several infections including malaria. In this study, we show that V(Y)9V delta 2 T cells proliferate when stimulated with supernatants from intraerythrocytic stages of Plasmodium falciparum cultures, suggesting that biologically relevant doses of phosphoantigens are excreted by the parasite. Next, we used Anopheles gambiae to investigate the immune-and redox-stimulating effects of HMBPP. We demonstrate a potent activation in vitro of all but one of the signaling pathways earlier implicated in the human V(Y)9V delta 2 T cell response, as p38, JNK and PI3K/Akt but not ERK were activated in the A. gambiae 4a3B cell line. Additionally, both HMBPP and the downstream endogenous metabolite isopentenyl pyrophosphate displayed antioxidant effects by promoting cellular tolerance to hydrogen peroxide challenge. When provided in the mosquito blood meal, HMBPP induced temporal changes in the expression of several immune genes. In contrast to meso-diaminopimelic acid containing peptidoglycan, HMBPP induced expression of dual oxidase and nitric oxide synthase, two key determinants of Plasmodium infection. Furthermore, temporal fluctuations in midgut bacterial numbers were observed. The multifaceted effects observed in this study indicates that HMBPP is an important elicitor in common for both Plasmodium and gut bacteria in the mosquito.

Ämnesord

NATURVETENSKAP  -- Biologiska vetenskaper -- Immunologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Immunology (hsv//eng)

Nyckelord

Medical Cell Biology
Medicinsk cellbiologi
molekylärgenetik
Molecular Genetics

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