Profiling of Saccharomyces cerevisiae transcription factors for engineering the resistance of yeast to lignocellulose-derived inhibitors in biomass conversion

• Background: Yeast transcription factors (TFs) involved in the regulation of multidrug resistance (MDR) were investigated in experiments with deletion mutants, transformants overexpressing synthetic genes encoding TFs, and toxic concentrations of lignocellulose-derived substances added to cultures as complex mixtures or as specific compounds, viz. coniferyl aldehyde, 5-hydroxymethylfurfural, and furfural. Results: In the presence of complex mixtures of toxic substances from spruce wood, transformants overexpressing YAP1 and STB5, TFs involved in oxidative stress response, exhibited enhanced relative growth rates amounting to 4.589 +/- 0.261 and 1.455 +/- 0.185, respectively. Other TFs identified as important for resistance included DAL81, GZF3, LEU3, PUT3, and WAR1. Potential overlapping functions of YAP1 and STB5 were investigated in experiments with permutations of deletions and overexpression of the two genes. YAP1 complemented STB5 with respect to resistance to 5-hydroxymethylfurfural, but had a distinct role with regard to resistance to coniferyl aldehyde as deletion of YAP1 rendered the cell incapable of resisting coniferyl aldehyde even if STB5 was overexpressed. Conclusions: We have investigated 30 deletion mutants and eight transformants overexpressing MDR transcription factors with regard to the roles the transcription factors play in the resistance to toxic concentrations of lignocel-lulose-derived substances. This work provides an overview of the involvement of thirty transcription factors in the resistance to lignocellulose-derived substances, shows distinct and complementary roles played by YAP1 and STB5, and offers directions for the engineering of robust yeast strains for fermentation processes based on lignocellulosic feedstocks.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Nyckelord

Lignocellulosic biomass conversion

Saccharomyces cerevisiae

Multidrug resistance

Transcription ctors

STB5

YAP1

LAVEAU T

1994

MOLECULAR & GENERAL GENETICS

V244

P501 cau-Danila A

2003

JOURNAL OF BIOLOGICAL CHEMISTRY

V278

P52641 i Z

1998

MOLECULAR MICROBIOLOGY

V29

P1307 reira Clara

2007

MOLECULAR MICROBIOLOGY

V66

P571 innen Steve

2017

MICROBIAL CELL FACTORIES

V16

mougrand N

2004

FEMS YEAST RESEARCH2nd International Meeting on Yeast Apoptosis

SEP

2003

olenice

SLOVAKIA

V5

P133 lshan Kailash

2007

EUKARYOTIC CELL

V6

P1933 vka Adnan

2011

BIOTECHNOLOGY AND BIOENGINEERING

V108

P2592 ye-Rowley WS

2003

PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY

VOL 73

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