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Effect of cobalt io...
Effect of cobalt ions on the interaction between macrophages and titanium
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- Pettersson, Mattias, 1972- (författare)
- Umeå universitet,Tandläkarutbildning,Prosthetic Dentistry,Umea Univ, Prosthet Dent, Umea, Sweden
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- Pettersson, Jean (författare)
- Uppsala universitet,Analytisk kemi
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- Molin Thorén, Margareta, 1954- (författare)
- Umeå universitet,Institutionen för odontologi,Umea Univ, Prosthet Dent, Umea, Sweden
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- Johansson, Anders (författare)
- Umeå universitet,Institutionen för odontologi,Umea Univ, Fac Med, Dept Odontol, Mol Periodontol, Umea, Sweden
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(creator_code:org_t)
- 2018-09-25
- 2018
- Engelska.
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Ingår i: Journal of Biomedical Materials Research. Part A. - : John Wiley & Sons. - 1549-3296 .- 1552-4965. ; :9, s. 2518-2530
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Abstract
Ämnesord
Stäng
- Inflammation and bone reduction around dental implants are described as periimplantitis and can be caused by an inflammatory response against bacterial products and toxins. Titanium (Ti) forms aggregates with serum proteins, which activate and cause release of the cytokine interleukin (IL-1β) from human macrophages. It was hypothesized that cobalt (Co) ions can interact in the formation of pro-inflammatory aggregates, formed by titanium. To test this hypothesis, we differentiated THP-1 cells into macrophages and exposed them to Ti ions alone or in combination with Co ions to investigate if IL-1β release and cytotoxicity were affected. We also investigated aggregate formation, cell uptake and human biopsies with inductively coupled plasma atomic emission spectroscopy (ICP-AES) and electron microscopy. Co at a concentration of 100 μM neutralized the IL-1β release from human macrophages and affected the aggregate formation. The aggregates formed by Ti could be detected in the cytosol of macrophages. In the presence of Co, the Ti-induced aggregates were located in the cytosol of the cultured macrophages, but outside the lysosomal structures. It is concluded that Co can neutralize the Ti-induced activation and release of active IL-1β from human macrophages in vitro. Also, serum proteins are needed for the formation of metal-protein aggregates in cell medium. Furthermore, the structures of the aggregates as well as the localization after cellular uptake differ if Co is present in a Ti solution. Phagocytized aggregates with a similar appearance seen in vitro with Ti present, were also visible in a sample from human peri-implant tissue.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
Nyckelord
- titanium
- cobalt
- interleukin-1β
- peri-implantitis
- aggregate formation
- odontologi
- Odontology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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