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Sökning: onr:"swepub:oai:DiVA.org:uu-205549" > Identification of C...

Identification of Candidate Serum Proteins for Classifying Well-Differentiated Small Intestinal Neuroendocrine Tumors

Darmanis, Spyros, (författare)
Uppsala universitet, Molekylära verktyg, Uppsala universitet, Science for Life Laboratory, SciLifeLab
Cui, Tao, (författare)
Uppsala universitet, Onkologisk endokrinologi
Drobin, Kimi, (författare)
KTH - Royal Institute of Technology, Stockholm, Sweden, Science for Life Laboratory, School of Biotechnology
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Li, Su-Chen, (författare)
Uppsala universitet, Onkologisk endokrinologi
Öberg, Kjell, (författare)
Uppsala universitet, Onkologisk endokrinologi, Uppsala universitet, Science for Life Laboratory, SciLifeLab
Nilsson, Peter, (författare)
KTH - Royal Institute of Technology, Stockholm, Sweden, Science for Life Laboratory, School of Biotechnology
Schwenk, Jochen M., (författare)
KTH - Royal Institute of Technology, Stockholm, Sweden, Science for Life Laboratory, School of Biotechnology
Giandomenico, Valeria, (författare)
Uppsala universitet, Onkologisk endokrinologi, Uppsala universitet, Science for Life Laboratory, SciLifeLab
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2013
Engelska.
Ingår i: PLoS ONE. - 1932-6203 .- 1932-6203. ; 8:11, s. e81712
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • <p><strong>Background</strong></p><p>Patients with well-differentiated small intestine neuroendocrine tumors (WD-SI-NET) are most often diagnosed at a metastatic stage of disease, which reduces possibilities for a curative treatment. Thus new approaches for earlier detection and improved monitoring of the disease are required.</p><p><strong>Materials and methods</strong></p><p>Suspension bead arrays targeting 124 unique proteins with antibodies from the Human Protein Atlas were used to profile biotinylated serum samples. Discoveries from a cohort of 77 individuals were followed up in a cohort of 132 individuals both including healthy controls as well as patients with untreated primary WD-SI-NETs, lymph node metastases and liver metastases.</p><p><strong>Results </strong></p><p>A set of 20 antibodies suggested promising proteins for further verification based on technically verified statistical significance. Proceeding, we assessed the classification performance in an independent cohort of patient serum, achieving, classification accuracy of up to 85% with different subsets of antibodies in respective pairwise group comparisons. The protein profiles of nine targets, namely IGFBP2, IGF1, SHKBP1, ETS1, IL1α, STX2, MAML3, EGR3 and XIAP were verified as significant contributors to tumor classification.</p><p><strong>Conclusions</strong></p><p>We propose new potential protein biomarker candidates for classifying WD-SI-NET at different stage of disease. Further evaluation of these proteins in larger sample sets and with alternative approaches is needed in order to further improve our understanding of their functional relation to WD-SI-NET and their eventual use in diagnostics.</p>

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci) (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Gastroenterologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)

Nyckelord

Endokrinologi och Diabetologi
Endocrinology and Diabetology
Molecular Biology
Molekylärbiologi
Molekylär bioteknik
Molecular Biotechnology
Oncology
Onkologi

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