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Sökning: onr:"swepub:oai:DiVA.org:uu-206561" > Keratinocytes and H...

Keratinocytes and Head and Neck Squamous Cell Carcinoma Cells Regulate Urokinase-type Plasminogen Activator and Plasminogen Activator Inhibitor-1 in Fibroblasts

Hakelius, Malin, (författare)
Uppsala universitet, Plastikkirurgi
Koskela, Anita (författare)
Ivarsson, Mikael (författare)
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Grenman, Reidar (författare)
Rubin, Kristofer, (författare)
Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi
Gerdin, Bengt, (författare)
Uppsala universitet, Plastikkirurgi
Nowinski, Daniel, (författare)
Uppsala universitet, Plastikkirurgi
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2013
Engelska.
Ingår i: Anticancer Research. - 0250-7005 .- 1791-7530. ; 33:8, s. 3113-3118
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • <p>Background: To investigate possible differences in the effects of soluble factors from oral squamous cell carcinoma (SCC) cells (UT-SCC-87) and normal oral keratinocytes (NOK) on fibroblast expression of genes involved in tumor stroma turnover. Materials and Methods: Transwell co-cultures with fibroblasts in collagen gels, and SCC cells or NOK in inserts were carried out. Fibroblast gene expression was measured with real-time polymerase chain reaction (PCR). Results: The expression of urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) was up-regulated in co-cultures with SCC cells but not with NOK. In contrast, both SCC cells and NOK regulated matrix metalloproteinase-1 (MMP1) and -3, and tissue inhibitor of metalloproteinases-2 (TIMP2) and -3 to a similar extent, while MMP2 and TIMP1 were largely unaffected. Interleukin 1 alpha (IL1 alpha) up-regulated both MMP1 and MMP3 and down-regulated PAI-1, TIMP2 and -3. Conclusion: SCC and NOK regulate fibroblast expression of genes involved in tumor stroma turnover differentially in vitro. These observations may contribute to a better understanding of the mechanisms behind extracellular matrix turnover in tumors.</p>

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  (hsv//swe)
MEDICAL AND HEALTH SCIENCES  (hsv//eng)

Nyckelord

Head and neck cancer
keratinocytes
differential regulation
PAI-1
uPA

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