Central nervous system stem/progenitor cells form neurons and peripheral glia after transplantation to the dorsal root ganglion.

• We asked whether neural stem/progenitor cells from the cerebral cortex of E14.5 enhanced green fluorescent protein transgenic mice are able to survive grafting and differentiate in the adult rat dorsal root ganglion. Neurospheres were placed in lumbar dorsal root ganglion cavities after removal of the dorsal root ganglia. Alternatively, dissociated neurospheres were injected into intact dorsal root ganglia. Enhanced green fluorescent protein-positive cells in the dorsal root ganglion cavity were located in clusters and expressed beta-III-tubulin or glial fibrillary acidic protein after 1 month, whereas after 3 months, surviving grafted cells expressed only glial fibrillary acidic protein. In the intact adult DRG, transplanted neural stem/progenitor cells surrounded dorsal root ganglion cells and fibers, and expressed glial but not neuronal markers. These findings show that central nervous system stem/progenitor cells can survive and differentiate into neurons and peripheral glia after xenotransplantation to the adult dorsal root ganglion.

Nyckelord

Actins/genetics

animals

cell differentiation/physiology

cells

cultured

Cerebral Cortex/*cytology/embryology

embryo

ganglia

spinal/*cytology/transplantation

gene expression/physiology

green fluorescent

Proteins/genetics

immunohistochemistry/methods

intermediate filament proteins/metabolism

mice

mice

inbred C57BL

transgenic

nerve tissue proteins/metabolism

neuroglia/*physiology

neurons/*physiology

stem cell transplantation/methods

stem cells/*physiology

time factors

tubulin/metabolism

nerve regeneration

neural stem/progenitor cell

satellite cell

Schwann cell

sensory neuron

spinal cord

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)