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Sökning: onr:"swepub:oai:DiVA.org:uu-245563" > U-251 revisited

U-251 revisited genetic drift and phenotypic consequences of long-term cultures of glioblastoma cells

Torsvik, Anja (författare)
Stieber, Daniel (författare)
Enger, Per Oyvind (författare)
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Golebiewska, Anna (författare)
Molven, Anders (författare)
Svendsen, Agnete (författare)
Westermark, Bengt, (författare)
Uppsala universitet, Neuroonkologi
Niclou, Simone P. (författare)
Olsen, Thale Kristin (författare)
Enger, Martha Chekenya (författare)
Bjerkvig, Rolf (författare)
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Uppsala universitet Medicinska och farmaceutiska vetenskapsområdet. Medicinska fakulteten. Institutionen för immunologi, genetik och patologi. Neuroonkologi. (creator_code:org_t)
2014
Engelska.
Ingår i: Cancer Medicine. - 2045-7634 .- 2045-7634. ; 3:4, s. 812-824
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • It is well known that in vitro subculture represents a selection pressure on cell lines, and over time this may result in a genetic drift in the cancer cells. In addition, long-term cultures harbor the risk of cross-contamination with other cell lines. The consequences may have major impact on experimental results obtained in various laboratories, where the cell lines no longer reflect the original tumors that they are supposed to represent. Much neglected in the scientific community is a close monitoring of cell cultures by regular phenotypic and genetic characterization. In this report, we present a thorough characterization of the commonly used glioblastoma (GBM) model U-251, which in numerous publications has been wrongly identified as U-373, due to an earlier cross-contamination. In this work, the original U-251 and three subclones of U-251, commonly referred to as U-251 or U-373, were analyzed with regard to their DNA profile, morphology, phenotypic expression, and growth pattern. By array comparative genomic hybridization (aCGH), we show that only the original low-passaged U-251 cells, established in the 1960s, maintain a DNA copy number resembling a typical GBM profile, whereas all long-term subclones lost the typical GBM profile. Also the long-term passaged subclones displayed variations in phenotypic marker expression and showed an increased growth rate in vitro and a more aggressive growth in vivo. Taken together, the variations in genotype and phenotype as well as differences in growth characteristics may explain different results reported in various laboratories related to the U-251 cell line.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

Nyckelord

aCGH
cell lines
cross-contamination
GBM
in vitro models
STR
U251
U373

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