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Sökning: onr:"swepub:oai:DiVA.org:uu-348263" > Virome characterisa...

Virome characterisation from Guthrie cards in children who later developed acute lymphoblastic leukaemia.

Bogdanovic, G, (författare)
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
Pou, C, (författare)
Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Barrientos-Somarribas, M, (författare)
Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
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Bjerkner, A, (författare)
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
Honkaniemi, E, (författare)
Department of Pediatrics, Södertälje Hospital, Stockholm, Swden
Allander, T, (författare)
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
Andersson, B, (författare)
Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
Gustafsson, B, (författare)
Department of Clinical Science, Intervention and Technology, CLINTEC, Karolinska Institutet, Stokholm, Sweden
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2016
Engelska.
Ingår i: British Journal of Cancer. - 0007-0920 .- 1532-1827. ; 115:8, s. 1008-1014
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • BACKGROUND: Some childhood acute lymphoblastic leukaemias (ALL) can be traced back to a prenatal origin, where a virus infection could be involved in the first pre-leukaemic clone development. The DNA virome of 95 children who later developed ALL was characterised from neonatal blood spots (NBS) using unbiased next-generation sequencing (NGS) and compared with the virome of 95 non-ALL controls.METHODS: DNA was individually extracted from the ALL-patients and controls, pooled, randomly amplified and sequenced using the Illumina MiSeq Sequencing System.RESULTS: Virus-like sequences identified in both groups mapped to human endogenous retroviruses and propionibacterium phage, considered a part of the normal microbial flora. Potential pathogens human herpesvirus type 6 (HHV-6) and parvovirus B19 were also identified, but only few samples in both ALL and controls tested positive by PCR follow-up.CONCLUSIONS: Unbiased NGS was employed to search for DNA from potential infectious agents in neonatal samples of children who later developed ALL. Although several viral candidates were identified in the NBS samples, further investigation by PCR suggested that these viruses did not have a major role in ALL development.

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MEDICIN OCH HÄLSOVETENSKAP  (hsv//swe)
MEDICAL AND HEALTH SCIENCES  (hsv//eng)

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