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Shotgun Environment...
Shotgun Environmental DNA, Pollen, and Macrofossil Analysis of Lateglacial Lake Sediments From Southern Sweden
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- Parducci, Laura, 1965- (författare)
- Uppsala universitet,Växtekologi och evolution
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- Greve Alsos, Inger (författare)
- Univ Tromso, Arctic Univ Norway, Tromso Museum, Tromso, Norway
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- Unneberg, Per (författare)
- Uppsala universitet,Molekylär evolution,Science for Life Laboratory, SciLifeLab
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- Pedersen, Mikkel W. (författare)
- Univ Cambridge, Dept Zool, Cambridge, England
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- Han, Lu (författare)
- Uppsala universitet,Institutionen för ekologi och genetik,Jilin Univ, Ancient DNA Lab, Coll Life Sci, Changchun, Jilin, Peoples R China
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- Lammers, Youri (författare)
- Univ Tromso, Arctic Univ Norway, Tromso Museum, Tromso, Norway
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- Salonen, J. Sakari (författare)
- Univ Helsinki, Dept Geosci & Geog, Helsinki, Finland
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- Väliranta, Minna M. (författare)
- Univ Helsinki, Ecosyst & Environm Res Programme, ECRU, Helsinki, Finland
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- Slotte, Tanja (författare)
- Stockholms universitet,Institutionen för ekologi, miljö och botanik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Ecol Environm & Plant Sci, Stockholm, Sweden;Sci Life Lab, Solna, Sweden
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- Wohlfarth, Barbara (författare)
- Stockholms universitet,Institutionen för geologiska vetenskaper,Stockholm Univ, Dept Geol Sci, Stockholm, Sweden;Stockholm Univ, Bolin Ctr Climate Res, Stockholm, Sweden
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(creator_code:org_t)
- 2019-06-20
- 2019
- Engelska.
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Ingår i: Frontiers in Ecology and Evolution. - : Frontiers Media SA. - 2296-701X. ; 7
- Relaterad länk:
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https://doi.org/10.3...
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https://www.frontier...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The lake sediments of Hasseldala Port in south-east Sweden provide an archive of local and regional environmental conditions similar to 14.5-9.5 ka BP (thousand years before present) and allow testing DNA sequencing techniques to reconstruct past vegetation changes. We combined shotgun sequencing with plant micro- and macrofossil analyses to investigate sediments dating to the Allerod (14.1-12.7 ka BP), Younger Dryas (12.7-11.7 ka BP), and Preboreal (<11.7 ka BP). Number of reads and taxa were not associated with sample age or organic content. This suggests that, beyond the initial rapid degradation, DNA is still present. The proportion of recovered plant DNA was low, but allowed identifying an important number of plant taxa, thus adding valid information on the composition of the local vegetation. Importantly, DNA provides a stronger signal of plant community changes than plant micro- and plant macrofossil analyses alone, since a larger number of new taxa were recorded in Younger Dryas samples. A comparison between the three proxies highlights differences and similarities and supports earlier findings that plants growing close to or within a lake are recorded by DNA. Plant macrofossil remains moreover show that tree birch was present close to the ancient lake since the Allerod; together with the DNA results, this indicates that boreal to subarctic climatic conditions also prevailed during the cold Younger Dryas interval. Increasing DNA reference libraries and enrichment strategies prior to sequencing are necessary to improve the potential and accuracy of plant identification using the shotgun metagenomic approach.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Multidisciplinär geovetenskap (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Geosciences, Multidisciplinary (hsv//eng)
Nyckelord
- environmental DNA
- ancient DNA
- shotgun sequencing (metagenomics)
- pollen
- macrofossils remains
- lake sediments
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Parducci, Laura, ...
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Greve Alsos, Ing ...
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Unneberg, Per
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Pedersen, Mikkel ...
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Han, Lu
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Lammers, Youri
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visa fler...
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Salonen, J. Saka ...
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Väliranta, Minna ...
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Slotte, Tanja
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Wohlfarth, Barba ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Geovetenskap och ...
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och Multidisciplinär ...
- Artiklar i publikationen
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Frontiers in Eco ...
- Av lärosätet
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Stockholms universitet
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Uppsala universitet