Multi-HLA class II tetramer analyses of citrulline-reactive T cells and early treatment response in rheumatoid arthritis

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: onr:"swepub:oai:DiVA.org:uu-414324" > Multi-HLA class II ...

1 av 1
Föregående post
Nästa post
Till träfflistan

Multi-HLA class II tetramer analyses of citrulline-reactive T cells and early treatment response in rheumatoid arthritis

Gerstner, Christina (författare): Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.

Turcinov, Sara (författare): Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.

Hensvold, Aase H. (författare): Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.

visa fler...

Chemin, Karine (författare): Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.

Uchtenhagen, Hannes (författare): Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.;BRI Virginia Mason, Translat Res Program, Seattle, WA USA.

Ramwadhdoebe, Tamara H. (författare): Univ Amsterdam, Amsterdam UMC, Dept Clin Immunol & Rheumatol, Amsterdam Infect & Immun Inst, Amsterdam, Netherlands.;Univ Amsterdam, Amsterdam UMC, Dept Expt Immunol, Amsterdam Infect & Immun Inst, Amsterdam, Netherlands.;Acad Med Ctr, Amsterdam Rheumatol & Immunol Ctr ARC, Amsterdam, Netherlands.

Dubnovitsky, Anatoly (författare): Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.

Kozhukh, Genadiy (författare): Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.

Rönnblom, Lars (författare): Uppsala universitet,Reumatologi,Science for Life Laboratory, SciLifeLab

Kwok, William W. (författare): BRI Virginia Mason, Translat Res Program, Seattle, WA USA.

Achour, Adnane (författare): Karolinska Inst, Dept Med Solna, Sci Life Lab, Stockholm, Sweden.;Karolinska Univ Hosp, Div Infect Dis, Stockholm, Sweden.

Catrina, Anca (författare): Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.

van Baarsen, Lisa G. M. (författare): Univ Amsterdam, Amsterdam UMC, Dept Clin Immunol & Rheumatol, Amsterdam Infect & Immun Inst, Amsterdam, Netherlands.;Univ Amsterdam, Amsterdam UMC, Dept Expt Immunol, Amsterdam Infect & Immun Inst, Amsterdam, Netherlands.;Acad Med Ctr, Amsterdam Rheumatol & Immunol Ctr ARC, Amsterdam, Netherlands.

Malmstrom, Vivianne (författare): Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.

Hensvold, AH (författare)

Ramwadhdoebe, TH (författare)

Uchtenhagen, H (författare)

Dubnovitsky, A (författare)

Achour, A (författare): Karolinska Institutet

Malmström, V (författare)

Gerstner, C (författare)

Turcinov, S (författare)

Kozhukh, G (författare)

Catrina, AI (författare)

Rönnblom, L (författare)

van Baarsen, LGM (författare)

Chemin, K (författare)

Kwok, WW (författare)

Catrina, A (författare): Karolinska Institutet

Ronnblom, L (författare)

Malmstrom, V (författare)

visa färre...

Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden;Karolinska Inst, Stockholm, Sweden. Karolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.;BRI Virginia Mason, Translat Res Program, Seattle, WA USA. (creator_code:org_t)

2020
2020
Engelska.
Ingår i: ; 21:1

Relaterad länk:: https://doi.org/10.1...; visa fler...; http://uu.diva-porta...; https://doi.org/10.1...; http://kipublication...; http://kipublication...; visa färre...

Tidskriftsartikel (refereegranskat)

Abstract Ämnesord

Stäng

Background HLA class II tetramers can be used for ex vivo enumeration and phenotypic characterisation of antigen-specific CD4+ T cells. They are increasingly applied in settings like allergy, vaccination and autoimmune diseases. Rheumatoid arthritis (RA) is a chronic autoimmune disorder for which many autoantigens have been described. Results Using multi-parameter flow cytometry, we developed a multi-HLA class II tetramer approach to simultaneously study several antigen specificities in RA patient samples. We focused on previously described citrullinated HLA-DRB1*04:01-restricted T cell epitopes from alpha-enolase, fibrinogen-beta, vimentin as well as cartilage intermediate layer protein (CILP). First, we examined inter-assay variability and the sensitivity of the assay in peripheral blood from healthy donors (n = 7). Next, we confirmed the robustness and sensitivity in a cohort of RA patients with repeat blood draws (n = 14). We then applied our method in two different settings. We assessed lymphoid tissue from seropositive arthralgia (n = 5) and early RA patients (n = 5) and could demonstrate autoreactive T cells in individuals at risk of developing RA. Lastly, we studied peripheral blood from early RA patients (n = 10) and found that the group of patients achieving minimum disease activity (DAS28 < 2.6) at 6 months follow-up displayed a decrease in the frequency of citrulline-specific T cells. Conclusions Our study demonstrates the development of a sensitive tetramer panel allowing simultaneous characterisation of antigen-specific T cells in ex vivo patient samples including RA 'at risk' subjects. This multi-tetramer approach can be useful for longitudinal immune-monitoring in any disease with known HLA-restriction element and several candidate antigens.