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Human eosinophil peroxidase : purification and characterization

Carlson, M (författare)
Uppsala universitet,Institutionen för medicinska vetenskaper
Peterson, C G (författare)
Uppsala universitet,Institutionen för medicinska vetenskaper
Venge, P (författare)
Uppsala universitet,Institutionen för medicinska vetenskaper
 (creator_code:org_t)
1985
1985
Engelska.
Ingår i: Journal of Immunology. - 0022-1767 .- 1550-6606. ; 134:3, s. 1875-1879
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • Human eosinophil peroxidase (EPO) was isolated from granules from granulocytes of a patient with hypereosinophilia. The granules were extracted by means of 0.2 M NaAc, pH 4.0. The purification steps included gel filtration chromatography on Sephadex G-75 superfine and ion-exchange chromatography on CM-Sephadex G-50. The purified protein showed one band on agarose-electrophoresis, a high peroxidase activity, and a 415-nm/280 nm ratio of 1.15. After reduction, EPO showed two bands on SDS-PAGE of m.w. 52,000 and 15,000, respectively. On gel filtration, the unreduced protein had a m.w. of approximately 77,000. Amino acid analyses showed a high content of arginine and aspartic acid. Monospecific antibodies to EPO were prepared in rabbits, and a specific radioimmunoassay was developed. There was an almost linear correlation between the content of EPO measured by the radioimmunoassay and the number of eosinophils in a mixed cell extract from reference material, indicating the eosinophil origin of EPO. The content of EPO was estimated to be 15.0 micrograms/10(6) eosinophils.

Nyckelord

Amino Acids/analysis
Antigen-Antibody Reactions
Chromatography; Gel
Eosinophil Peroxidase
Eosinophilia/enzymology
Eosinophils/*enzymology
Humans
Immunodiffusion
Leukocyte Count
Molecular Weight
Peroxidases/analysis/*isolation & purification/metabolism
Radioimmunoassay
Research Support; Non-U.S. Gov't
MEDICINE
MEDICIN

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Av författaren/redakt...
Carlson, M
Peterson, C G
Venge, P
Artiklar i publikationen
Journal of Immun ...
Av lärosätet
Uppsala universitet

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