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A conserved KASH do...
A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis
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Morimoto, Akihiro (författare)
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- Shibuya, Hiroki, 1985 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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Zhu, Xiaoqiang (författare)
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Kim, Jihye (författare)
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Ishiguro, Kei Ichiro (författare)
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Han, Min (författare)
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Watanabe, Yoshinori (författare)
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(creator_code:org_t)
- 2012-07-23
- 2012
- Engelska.
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Ingår i: Journal of Cell Biology. - : Rockefeller University Press. - 0021-9525 .- 1540-8140. ; 198, s. 165-172
- Relaterad länk:
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http://jcb.rupress.o...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- n yeasts and worms, KASH (Klarsicht/ANC-1/Syne/ homology) domain and SUN (Sad-1/UNC-84) domain nuclear envelope (NE) proteins play a crucial role in meiotic chromosome movement and homologue pair-ing. However, although the vertebrate SUN domain pro-tein SUN1 is involved in these processes, its partner has remained identified. Based on subcellular localiza-tion screening in mouse spermatocytes, we identified a novel germ cell-specific protein, KASH5, that localized exclusively at telomeres from the leptotene to diplotene stages in both spermatocytes and oocytes. KASH5 pos-sesses hitherto unknown KASH-related sequences that directly interacted with SUN1 and mediated telomere loca-lization. Thus, KASH5 is a mammalian meiosis-specific KASH domain protein. We show that meiotic chromo-some movement depended on microtubules and that KASH5 interacted with the microtubule-associated dynein-dynactin complex. These results suggest that KASH5 connects the telomere-associated SUN1 protein to the cytoplasmic force-generating mechanism involved in meiotic chromosome movement. Our study strongly sug-gests that the meiotic homologue-pairing mechanism mediated by the SUN-KASH NE bridge is highly con-served among eukaryotes. © 2012 Morimoto et al.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
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