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Galectin-3 type-C s...
Galectin-3 type-C self-association on neutrophil surfaces; The carbohydrate recognition domain regulates cell function
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- Sundqvist, Martina (författare)
- University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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- Welin, Amanda, 1983 (författare)
- University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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- Elmwall, Jonas (författare)
- University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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- Osla, Veronica (författare)
- University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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- Nilsson, Ulf J. (författare)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Leffler, Hakon (författare)
- Lund University,Lunds universitet,Avdelningen för mikrobiologi, immunologi och glykobiologi - MIG,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Microbiology, Immunology and Glycobiology - MIG,Department of Laboratory Medicine,Faculty of Medicine
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- Bylund, Johan, 1975 (författare)
- University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för odontologi,Institute of Odontology
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- Karlsson, Anna, 1967 (författare)
- University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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(creator_code:org_t)
- 2018
- 2018
- Engelska.
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Ingår i: Journal of Leukocyte Biology. - 0741-5400. ; 103:2, s. 341-353
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Abstract
Ämnesord
Stäng
- Galectin-3 is an endogenous β-galactoside-binding lectin comprising a carbohydrate recognition domain (CRD) linked to a collagen-like N-domain. Both domains are required for galectin-3 to induce cellular effects; a C-terminal fragment of galectin-3, galectin-3C, containing the CRD but lacking the N-domain, binds cell surface glycoconjugates but does not induce cellular effects since cross-linking promoted by the N-domain is thought to be required. Instead, galectin-3C is proposed to antagonize the effects of galectin-3 by competing for binding sites. The aim of this study was to investigate the effects of galectin-3C on galectin-3 interactions with human neutrophils. Recombinant galectin-3C inhibited galectin-3-induced production of reactive oxygen species in primed neutrophils. Surprisingly, this inhibition was not due to competitive inhibition of galectin-3 binding to the cells. In contrast, galectin-3C potentiated galectin-3 binding, in line with emerging evidence that galectin-3 can aggregate not only through the N-domain but also through the CRD. The cell surface interaction between galectin-3C and galectin-3 was corroborated by colocalization of fluorescently labeled galectin-3 and galectin-3C. Galectin-3C can be generated in vivo through cleavage of galectin-3 by proteases. Indeed, in circulation, galectin-3 and galectin-3C were both attached to the cell surface of neutrophils, which displayed great capacity to bind additional galectin-3 and galectin-3C. In conclusion, galectin-3C enhances galectin-3 binding to neutrophils by nonactivating type-C self-association, in parallel to inhibiting neutrophil activation by galectin-3 (induced by type-N self-association). This implicates type-C self-association as a termination system for galectin-3-induced cell activation, with the purpose of avoiding oxidant-dependent tissue damage. ©2018 Society for Leukocyte Biology
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reumatologi och inflammation (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Rheumatology and Autoimmunity (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- carbohydrate recognition domain
- galectin
- neutrophils
- priming
- ROS production
- calcium
- fluorescein isothiocyanate
- galectin 3
- galectin 3c
- matrix metalloproteinase
- proteinase
- reactive oxygen metabolite
- recombinant galectin 3
- recombinant galectin 3c
- recombinant protein
- reduced nicotinamide adenine dinucleotide phosphate oxidase
- serine proteinase
- unclassified drug
- amino terminal sequence
- Article
- binding affinity
- calcium cell level
- calcium signaling
- carboxy terminal sequence
- cell interaction
- cell surface
- cellular distribution
- circulation
- competitive inhibition
- controlled study
- enzyme activation
- ex vivo study
- flow cytometry
- fluorescence
- human
- human cell
- leukocyte activation
- leukocyte adherence
- leukocyte function
- neutrophil
- nonhuman
- normal human
- oxidative stress
- priority journal
- protein assembly
- protein binding
- protein cleavage
- protein degradation
- protein protein interaction
- protein structure
- Carbohydrate recognition domain
- Galectin
- Neutrophils
- Priming
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Sundqvist, Marti ...
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Welin, Amanda, 1 ...
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Elmwall, Jonas
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Osla, Veronica
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Nilsson, Ulf J.
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Leffler, Hakon
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Bylund, Johan, 1 ...
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Karlsson, Anna, ...
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