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Optimization of FIB...
Optimization of FIB-SEM Tomography and Reconstruction for Soft, Porous, and Poorly Conducting Materials
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- Fager, Cecilia, 1990 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden
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- Röding, Magnus, 1984 (författare)
- RISE,Jordbruk och livsmedel
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- Olsson, Anna, 1985 (författare)
- AstraZeneca AB,AstraZeneca R&D Mölndal, Sweden
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visa fler...
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- Lorén, Niklas, 1970 (författare)
- RISE,Jordbruk och livsmedel,Chalmers University of Technology, Sweden
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- von Corswant, C. (författare)
- AstraZeneca AB,AstraZeneca R&D Mölndal, Sweden
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- Särkkä, Aila, 1962 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper,Department of Mathematical Sciences,Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden; University of Gothenburg, Sweden
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- Olsson, Eva, 1960 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden
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visa färre...
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(creator_code:org_t)
- 2020
- 2020
- Engelska.
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Ingår i: Microscopy and Microanalysis. - 1431-9276 .- 1435-8115. ; 26:4, s. 837-845
- Relaterad länk:
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https://research.cha... (primary) (free)
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://research.cha...
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https://research.cha...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Tomography using a focused ion beam (FIB) combined with a scanning electron microscope (SEM) is well-established for a wide range of conducting materials. However, performing FIB-SEM tomography on ion- and electron-beam-sensitive materials as well as poorly conducting soft materials remains challenging. Some common challenges include cross-sectioning artifacts, shadowing effects, and charging. Fully dense materials provide a planar cross section, whereas pores also expose subsurface areas of the planar cross-section surface. The image intensity of the subsurface areas gives rise to overlap between the grayscale intensity levels of the solid and pore areas, which complicates image processing and segmentation for three-dimensional (3D) reconstruction. To avoid the introduction of artifacts, the goal is to examine porous and poorly conducting soft materials as close as possible to their original state. This work presents a protocol for the optimization of FIB-SEM tomography parameters for porous and poorly conducting soft materials. The protocol reduces cross-sectioning artifacts, charging, and eliminates shadowing effects. In addition, it handles the subsurface and grayscale intensity overlap problems in image segmentation. The protocol was evaluated on porous polymer films which have both poor conductivity and pores. 3D reconstructions, with automated data segmentation, from three films with different porosities were successfully obtained.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Annan materialteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Other Materials Engineering (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Textil-, gummi- och polymermaterial (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Textile, Rubber and Polymeric Materials (hsv//eng)
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
Nyckelord
- 3D
- focused ion beam
- poorly conducting material
- scanning electron
- microscopy
- soft material
- tomography
- scanning-electron-microscopy
- biological samples
- radiation-damage
- beam
- damage
- 3d
- tem
- resolution
- images
- cells
- Materials Science
- Microscopy
- 3D
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Fager, Cecilia, ...
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Röding, Magnus, ...
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Olsson, Anna, 19 ...
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Lorén, Niklas, 1 ...
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von Corswant, C.
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Särkkä, Aila, 19 ...
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visa fler...
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Olsson, Eva, 196 ...
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visa färre...
- Om ämnet
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Materialteknik
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och Annan materialte ...
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- TEKNIK OCH TEKNOLOGIER
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TEKNIK OCH TEKNO ...
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och Materialteknik
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och Textil gummi och ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Materialkemi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Fysik
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och Annan fysik
- Artiklar i publikationen
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Microscopy and M ...
- Av lärosätet
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Göteborgs universitet
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Chalmers tekniska högskola
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RISE