Progenitor cell injury after irradiation to the developing brain can be modulated by mild hypothermia or hyperthermia

• Ionizing radiation induced acute cell death in the dentate gyrus subgranular zone (SGZ) and the subventricular zone (SVZ). Hypomyelination was also observed. The effects of mild hypothermia and hyperthermia for 4 h after irradiation (IR) were studied in postnatal day 9 rats. One hemisphere was irradiated with a single dose of 8 Gy and animals were randomized to normothermia (rectal temperature 36 degrees C for 4 h), hypothermia (32 degrees C for 4 h) or hyperthermia (39 degrees C for 4 h). Cellular injury, e.g. chromatin condensation and nitrotyrosine formation, appeared to proceed faster when the body temperature was higher. Caspase-3 activation was more pronounced in the hyperthermia group and nuclear translocation of p53 was less pronounced in the hypothermia group 6 h after IR. In the SVZ the loss of nestin-positive progenitors was more pronounced (48%) and the size was smaller (45%) in the hyperthermia group 7 days post-IR. Myelination was not different after hypo- or hyperthermia. This is the first report to demonstrate that hypothermia may be beneficial and that hyperthermia may aggravate the adverse side-effects after radiation therapy to the developing brain.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Physiology (hsv//eng)

Nyckelord

Animals

Animals

Newborn

Apoptosis/physiology/radiation effects

Body Temperature/*physiology

Brain/growth & development/physiopathology/*radiation effects

Brain Damage

Chronic/physiopathology/prevention & control/*therapy

Caspases/metabolism

Dentate Gyrus/growth & development/physiopathology/radiation effects

Female

Hyperthermia

Induced/adverse effects

Hypothermia

Induced

Intermediate Filament Proteins/metabolism

Male

Nerve Degeneration/physiopathology/prevention & control/therapy

Nerve Tissue Proteins/metabolism

Neurons/physiology/*radiation effects

Radiation Injuries

control/*therapy

Radiation

Ionizing

Rats

Rats

Wistar

Research Support

Non-U.S. Gov't

Stem Cells/physiology/*radiation effects

Tumor Suppressor Protein p53/metabolism

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)