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Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay

Aits, Sonja, (författare)
Danish Cancer Society Research Center
Kricker, Jennifer, (författare)
University of Iceland, Danish Cancer Society Research Center
Liu, Bin, (författare)
Danish Cancer Society Research Center
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Ellegaard, Anne Marie, (författare)
Danish Cancer Society Research Center
Hämälistö, Saara, (författare)
Danish Cancer Society Research Center
Tvingsholm, Siri, (författare)
Danish Cancer Society Research Center
Corcelle-Termeau, Elisabeth, (författare)
Danish Cancer Society Research Center
Høgh, Søren, (författare)
Danish Cancer Society Research Center
Farkas, Thomas, (författare)
Danish Cancer Society Research Center
Jonassen, Anna Holm, (författare)
Danish Cancer Society Research Center
Gromova, Irina, (författare)
Danish Cancer Society Research Center
Mortensen, Monika, (författare)
Danish Cancer Society Research Center
Jäättelä, Marja, (författare)
Danish Cancer Society Research Center
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Ingår i: Autophagy. - Landes Bioscience. - 1554-8627. ; 11:8, s. 1408-1424
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
  • Lysosomal membrane permeabilization (LMP) contributes to tissue involution, degenerative diseases, and cancer therapy. Its investigation has, however, been hindered by the lack of sensitive methods. Here, we characterize and validate the detection of galectin puncta at leaky lysosomes as a highly sensitive and easily manageable assay for LMP. LGALS1/galectin-1 and LGALS3/ galectin-3 are best suited for this purpose due to their widespread expression, rapid translocation to leaky lysosomes and availability of high-affinity antibodies. Galectin staining marks individual leaky lysosomes early during lysosomal cell death and is useful when defining whether LMP is a primary or secondary cause of cell death. This sensitive method also reveals that cells can survive limited LMP and confirms a rapid formation of autophagic structures at the site of galectin puncta. Importantly, galectin staining detects individual leaky lysosomes also in paraffin-embedded tissues allowing us to demonstrate LMP in tumor xenografts in mice treated with cationic amphiphilic drugs and to identify a subpopulation of lysosomes that initiates LMP in involuting mouse mammary gland. The use of ectopic fluorescent galectins renders the galectin puncta assay suitable for automated screening and visualization of LMP in live cells and animals. Thus, the lysosomal galectin puncta assay opens up new possibilities to study LMP in cell death and its role in other cellular processes such as autophagy, senescence, aging, and inflammation.


MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)


Breast involution
C. elegans
Cell death

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