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Influence of core d...
Influence of core divisome proteins on cell division in Streptomyces venezuelae ATCC 10712
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- Cantlay, Stuart (författare)
- Present address: Department of Biological Sciences, West Liberty University, West Liberty, WV 26074, USA;Department of Biological Sciences, Duquesne University, Pittsburgh, PA 15282, USA
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- Sen, Beer Chakra (författare)
- Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Mikrobiologigruppen,Forskargrupper vid Lunds universitet,Molecular Cell Biology,Department of Biology,Faculty of Science,Microbiology Group,Lund University Research Groups,Department of Biology, Lund University, 223 62 Lund, Sweden
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- Flärdh, Klas (författare)
- Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Mikrobiologigruppen,Forskargrupper vid Lunds universitet,Molecular Cell Biology,Department of Biology,Faculty of Science,Microbiology Group,Lund University Research Groups,Department of Biology, Lund University, 223 62 Lund, Sweden
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- McCormick, Joseph R. (författare)
- Department of Biological Sciences, Duquesne University, Pittsburgh, PA 15282, USA
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(creator_code:org_t)
- Microbiology Society, 2021
- 2021
- Engelska.
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Ingår i: Microbiology (Reading, England). - : Microbiology Society. - 1465-2080 .- 1350-0872. ; 167:2
- Relaterad länk:
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https://portal.resea... (primary) (free)
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http://dx.doi.org/10...
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https://www.ncbi.nlm...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The sporulating, filamentous soil bacterium Streptomyces venezuelae ATCC 10712 differentiates under submerged and surface growth conditions. In order to lay a solid foundation for the study of development-associated division for this organism, a congenic set of mutants was isolated, individually deleted for a gene encoding either a cytoplasmic (i.e. ftsZ) or core inner membrane (i.e. divIC, ftsL, ftsI, ftsQ, ftsW) component of the divisome. While ftsZ mutants are completely blocked for division, single mutants in the other core divisome genes resulted in partial, yet similar, blocks in sporulation septum formation. Double and triple mutants for core divisome membrane components displayed phenotypes that were similar to those of the single mutants, demonstrating that the phenotypes were not synergistic. Division in this organism is still partially functional without multiple core divisome proteins, suggesting that perhaps other unknown lineage-specific proteins perform redundant functions. In addition, by isolating an ftsZ2p mutant with an altered -10 region, the conserved developmentally controlled promoter was also shown to be required for sporulation-associated division. Finally, microscopic observation of FtsZ-YFP dynamics in the different mutant backgrounds led to the conclusion that the initial assembly of regular Z rings does not per se require the tested divisome membrane proteins, but the stability of Z rings is dependent on the divisome membrane components tested. The observation is consistent with the interpretation that Z ring instability likely results from and further contributes to the observed defects in sporulation septation in mutants lacking core divisome proteins.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- divisome
- morphological development
- septation
- spores
- sporulation
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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