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Sökning: onr:"swepub:oai:lup.lub.lu.se:8ad4fe2e-6e8b-49d7-ada7-58b32a4f0841" > The effects of MEK1...

The effects of MEK1/2 inhibition on cigarette smoke exposure-induced ET receptor upregulation in rat cerebral arteries

Cao, Lei, (författare)
Lunds universitet, Lund University, Medicinska fakulteten, Faculty of Medicine, Institutionen för kliniska vetenskaper, Lund, Department of Clinical Sciences, Lund, Sektion I-II, Division I-II, Medicin, Lund, Medicine, Lund, Experimentell kärlforskning, Experimental Vascular Research, Xi'an Jiaotong University
Ping, Na Na, (författare)
Xi'an Jiaotong University
Cao, Yong Xiao, (författare)
Xi'an Jiaotong University
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Li, Wei, (författare)
Xi'an Jiaotong University
Cai, Yan, (författare)
Xi'an Jiaotong University
Warfvinge, Karin, (författare)
Lunds universitet, Lund University, Medicinska fakulteten, Faculty of Medicine, Institutionen för kliniska vetenskaper, Lund, Department of Clinical Sciences, Lund, Sektion I-II, Division I-II, Medicin, Lund, Medicine, Lund, Experimentell kärlforskning, Experimental Vascular Research
Edvinsson, Lars, (författare)
Lunds universitet, Lund University, Medicinska fakulteten, Faculty of Medicine, Institutionen för kliniska vetenskaper, Lund, Department of Clinical Sciences, Lund, Sektion I-II, Division I-II, Medicin, Lund, Medicine, Lund, Experimentell kärlforskning, Experimental Vascular Research
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Lunds universitet Experimentell kärlforskning. (creator_code:org_t)
2016
Engelska 9 s.
Ingår i: Toxicology and Applied Pharmacology. - Academic Press. - 0041-008X. ; 304, s. 70-78
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Cigarette smoking, a major stroke risk factor, upregulates endothelin receptors in cerebral arteries. The present study examined the effects of MEK1/2 pathway inhibition on cigarette smoke exposure-induced ET receptor upregulation. Rats were exposed to the secondhand smoke (SHS) for 8 weeks followed by intraperitoneal injection of MEK1/2 inhibitor, U0126 for another 4 weeks. The urine cotinine levels were assessed with high-performance liquid chromatography. Contractile responses of isolated cerebral arteries were recorded by a sensitive wire myograph. The mRNA and protein expression levels of receptor and MEK/ERK1/2 pathway molecules were examined by real-time PCR and Western blotting, respectively. Cerebral artery receptor localization was determined with immunohistochemistry. The results showed the urine cotinine levels from SHS exposure group were significantly higher than those from the fresh group. In addition, the MEK1/2 inhibitor, U0126 significantly reduced SHS exposure-increased ETA receptor mRNA and protein levels as well as contractile responses mediated by ETA receptors. The immunoreactivity of increased ETA receptor expression was primarily cytoplasmic in smooth muscle cells. In contrast, ETB receptor was noted in endothelial cells. However, the SHS-induced decrease in endothelium-dependent relaxation was unchanged after U0126 treatment. Furthermore, SHS increased the phosphorylation of MEK1/2 and ERK1/2 protein in cerebral arteries. By using U0126 could inhibit the phosphorylated ERK1/2 protein but not MEK1/2. Taken together, our data show that treatment with MEK1/2 pathway inhibitor offsets SHS exposure-induced ETA receptor upregulation in rat cerebral arteries.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)

Nyckelord

Cerebral artery
Cigarette smoke
Endothelin receptor
MEK1/2
Rat
Receptor upregulation

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