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Sökning: onr:"swepub:oai:lup.lub.lu.se:97b7c643-cbfc-44fe-aca2-0b15f8e896c5" > Differentially ampl...

Differentially amplified chromosome 12 sequences in low- and high-grade osteosarcoma.

Gisselsson Nord, David, (författare)
Lunds universitet, Lund University, Medicinska fakulteten, Faculty of Medicine, Institutionen för laboratoriemedicin, Department of Laboratory Medicine, Avdelningen för klinisk genetik, Division of Clinical Genetics
Pålsson, Eva, (författare)
Lunds universitet, Lund University, Medicinska fakulteten, Faculty of Medicine, Institutionen för laboratoriemedicin, Department of Laboratory Medicine, Avdelningen för klinisk genetik, Division of Clinical Genetics
Höglund, Mattias, (författare)
Lunds universitet, Lund University, Medicinska fakulteten, Faculty of Medicine, Institutionen för laboratoriemedicin, Department of Laboratory Medicine, Avdelningen för klinisk genetik, Division of Clinical Genetics
visa fler...
Domanski, Henryk, (författare)
Lunds universitet, Lund University, Medicinska fakulteten, Faculty of Medicine, Institutionen för kliniska vetenskaper, Lund, Department of Clinical Sciences, Lund, Tumörmikromiljö, Tumor microenvironment, Sektion I, Section I
Mertens, Fredrik, (författare)
Lunds universitet, Lund University, Medicinska fakulteten, Faculty of Medicine, Institutionen för laboratoriemedicin, Department of Laboratory Medicine, Avdelningen för klinisk genetik, Division of Clinical Genetics
Pandis, Nikos (författare)
Sciot, Raf (författare)
Dal Cin, Paola (författare)
Bridge, Julia A (författare)
Mandahl, Nils, (författare)
Lunds universitet, Lund University, Medicinska fakulteten, Faculty of Medicine, Institutionen för laboratoriemedicin, Department of Laboratory Medicine, Avdelningen för klinisk genetik, Division of Clinical Genetics
visa färre...
2002
Engelska.
Ingår i: Genes, Chromosomes and Cancer. - John Wiley and Sons Inc.. - 1045-2257. ; 33:2, s. 133-140
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Most osteosarcomas are highly aggressive malignancies characterized by a complex pattern of chromosome abnormalities. However, a subgroup of low-grade, parosteal tumors exhibits a relatively simple aberration pattern dominated by ring chromosomes carrying amplified material from chromosome 12. To assess whether sequences from this chromosome were differentially amplified in low- and high-grade osteosarcomas, copy numbers of the CCND2, ETV6, KRAS2, and D12S85 regions in 12p and the MDM2 region in 12q were evaluated by interphase or metaphase fluorescence in situ hybridization (FISH) in 24 osteosarcomas. Amplification of MDM2 was detected in all five low-grade and four high-grade osteosarcomas, all of which showed ring chromosomes. An overrepresentation of 12p sequences was found in 1/5 low-grade and in 9/19 high-grade tumors. Multicolor single-copy FISH analysis of metaphase cells from six high-grade tumors showed that extra 12p material either occurred together with MDM2 in ring chromosomes or was scattered over the genome as a result of complex structural rearrangements. Most tumors (8/10) not containing amplification of the assessed chromosome 12 loci exhibited a nondiploid pattern at evaluation with probes for centromeric alpha satellite sequences. These findings indicate that gain of sequences from the short arm of chromosome 12 could be a possible genetic pathway in the development of aggressive osteosarcoma.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska grundvetenskaper -- Medicinsk genetik (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Medical Genetics (hsv//eng)

Nyckelord

Karyotyping
Interphase/genetics
Fluorescence
In Situ Hybridization
Human
Gene Amplification/*genetics
Female
Comparative Study
Pair 12/*genetics
Chromosomes
Chromosome Banding
Child
Bone Neoplasms/*genetics/pathology
Base Sequence
Adolescence
Adult
Male
Metaphase/genetics
Middle Age
Osteosarcoma/*genetics/pathology
Support
Non-U.S. Gov't
Tumor Cells
Cultured

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