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Sökning: onr:"swepub:oai:lup.lub.lu.se:9cb02667-7ea3-45d6-96ac-2e6bca3ba609" > The host defense pe...

  • Dahl, Sara,Forskargrupper vid Lunds universitet, Lund University Research Groups, Lunds universitet, Lund University, Kärlfysiologi, Vascular Physiology (författare)

The host defense peptide LL-37 triggers release of nucleic acids from human mast cells

  • Artikel/kapitelEngelska2018

Förlag, utgivningsår, omfång ...

  • 7 s.

Nummerbeteckningar

  • LIBRIS-ID:oai:lup.lub.lu.se:9cb02667-7ea3-45d6-96ac-2e6bca3ba609
  • 30308233pmid
  • 85054627565scopus
  • 10.1016/j.peptides.2018.10.001doi

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Klassifikation

  • Ämneskategori:art swepub-publicationtype
  • Ämneskategori:ref swepub-contenttype

Anmärkningar

  • Published
  • 5
  • The human host defense peptide LL-37 possesses antimicrobial activity but also affects host cell function and viability. Mast cells are involved in innate immunity but no data have been presented on effects of LL-37 on human mast cell viability and export of nucleic acids. Here, we demonstrated by immunofluorescence microscopy that synthesized LL-37 was internalized by human LAD2 mast cells and detected both in cytoplasm and nucleus. Treatment with high (4 and 10 μM) but not low (1 μM) concentrations of LL-37 for 4 h reduced cell viability assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Stimulation with 10 μM LL-37 for 4 h enhanced export of nucleic acids, total protein and lactate dehydrogenase (LDH), suggesting that both nuclear and plasma membranes are permeabilized by LL-37. Although LL-37 triggered release of nucleic acids, no extracellular trap-like structures were observed by laser scanning confocal microscopy of cells incubated with the plasma membrane impermeable nucleic acid fluorophore SYTOX-Green, indicating that LL-37 promotes export of nucleic acids but not formation of extracellular traps. On the other hand, phorbol-12-myristate-13-acetate (PMA), which is a well-known inducer of extracellular traps, stimulated export of nucleic acids and also formation of extracellular trap-like structures. However, PMA had no effect on export of either total protein or LDH. Hence, LL-37 and PMA seem to stimulate export of nucleic acids from LAD2 mast cells through different pathways. In conclusion, we demonstrate that LL-37 triggers release of nucleic acids from human mast cells but not the formation of extracellular trap-like structures.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Anders, Emma,Forskargrupper vid Lunds universitet, Lund University Research Groups, Lunds universitet, Lund University, Kärlfysiologi, Vascular Physiology(SwePub:) (författare)
  • Gidlöf, Olof,Forskargrupper vid Lunds universitet, Lund University Research Groups, Lunds universitet, Lund University, Molekylär kardiologi, Molecular Cardiology(SwePub:) (författare)
  • Svensson, Daniel,Forskargrupper vid Lunds universitet, Lund University Research Groups, Lunds universitet, Lund University, Kärlfysiologi, Vascular Physiology, Karolinska Institutet(SwePub:) (författare)
  • Nilsson, Bengt Olof,Forskargrupper vid Lunds universitet, Lund University Research Groups, Lunds universitet, Lund University, Kärlfysiologi, Vascular Physiology(SwePub:) (författare)
  • Dahl, S(SwePub:ki) (författare)
  • Anders, E(SwePub:ki) (författare)
  • Gidlof, O(SwePub:ki) (författare)
  • Svensson, D,Karolinska Institutet(SwePub:ki)48b7357dd6ec7c912e33e51da73d2bd6 (författare)
  • Nilsson, BO(SwePub:ki) (författare)

Sammanhörande titlar

  • Ingår i:PeptidesElsevier0196-9781109, s. 39-45

Internetlänk

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  • Peptides (Sök värdpublikationen i LIBRIS)

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