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A pTalpha-negative ...
A pTalpha-negative subpopulation of CD25+ TN thymocytes revealed by a transgenic marker.
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Leandersson, Karin (författare)
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Mårtensson, A (författare)
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Mertsching, E (författare)
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Winkler, T (författare)
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Ceredig, R (författare)
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Mårtensson, I-L (författare)
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- Ivars, Fredrik (författare)
- Lund University,Lunds universitet,Immunologi,Forskargrupper vid Lunds universitet,Immunology,Lund University Research Groups
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(creator_code:org_t)
- 2002-03-15
- 2002
- Engelska.
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Ingår i: Scandinavian Journal of Immunology. - : Wiley. - 1365-3083 .- 0300-9475. ; 55:2, s. 119-128
- Relaterad länk:
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http://www.ncbi.nlm....
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We have recently generated 5'lambda5-huTAC mice, which express the human CD25 (huTAC) gene under the control of the 5'-flanking region of the mouse lambda5-gene. The huTAC-transgene was expressed in pre-B cells but neither in mature B cells nor in T cells of these mice. In this report we demonstrate that the transgene is also transiently expressed by adult CD25+ CD3-CD4-CD8- (triple negative, TN) thymocytes and in fetal thymocytes. The huTAC+, in contrast to the huTAC- subpopulation of the CD44+CD25+ TN cells, was unexpectedly found not to express the pTalpha-gene. Still the huTAC+CD44+CD25+ TN cells reconstituted the development of both alphabeta and gammadelta lineage cells equally efficiently as the pTalpha-expressing huTAC- fraction, demonstrating that this pTalpha-negative subpopulation contained precursors for both T-cell lineages. Single cell reverse transcription-polymerase chain reaction (RT-PCR) experiments demonstrated that also in normal mice only a fraction of CD44+CD25+ and CD44-CD25+ TN cells expressed this gene. Taken together, these data indicate that huTAC transgene expression revealed a truly pTalpha-negative fraction of the CD44+CD25+ TN cells. The observation that not all precursors in the CD25+ TN population express the pTalpha-gene has important implications for the understanding of early T-cell development and T-cell lineage commitment.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Antigen
- T-Cell
- gamma-delta/metabolism
- Receptors
- Interleukin-2/*genetics/*metabolism
- alpha-beta/metabolism
- Transgenic
- Mice
- Membrane Glycoproteins/*genetics
- Liver/cytology/immunology
- Hematopoietic Stem Cells/immunology
- Genetic Markers
- Reporter
- Genes
- Gene Expression
- Fetus/cytology/immunology
- B-Lymphocytes/immunology
- CD44/metabolism
- Animal
- Antigens
- T-Lymphocyte Subsets/*immunology
- Thymus Gland/cytology/immunology
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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