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Sökning: onr:"swepub:oai:lup.lub.lu.se:d4716564-e0a1-45f3-b8f9-13c927ba566c" > Sample preparation ...

Sample preparation for single-cell whole chromosome analysis

Beech, Jason P. (författare)
Lund University,Lunds universitet,NanoLund,Annan verksamhet,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
Adolfsson, Karl (författare)
Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
Holm, Stefan (författare)
Lund University,Lunds universitet,NanoLund,Annan verksamhet,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
visa fler...
Yadegari, Farnaz (författare)
Lund University
Freitag, Camilla (författare)
University of Gothenburg
Fritzsche, Joachim (författare)
University of Gothenburg
Mir, Kalim U. (författare)
University of Oxford
Tegenfeldt, Jonas O. (författare)
University of Gothenburg,Lund University,Lunds universitet,NanoLund,Annan verksamhet,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
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 (creator_code:org_t)
Chemical and Biological Microsystems Society, 2012-01-01
Engelska 2 s.
Ingår i: 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012,Okinawa, Japan,2012-10-28 - 2012-11-01. - : Chemical and Biological Microsystems Society. ; , s. 998-999
  • Konferensbidrag (refereegranskat)
Abstract Ämnesord
Stäng  
  • In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DNA in one piece and transferring it to a nano-channel for visualisation. In order to achieve control over the bacteria we encapsulate them in agarose, using flow focusing. The encapsulated bacteria can then be transported in microchannels to proximity with the nanochannels and then chemically lysis can be performed. Following lysis the intact genome can be extracted and transferred to the meandering nanochannel for analysis. We believe this device holds the potential to significantly decrease analysis times for single cell, whole genome analysis with the potential of opening up for automated, high-throughput genome analysis in microfluidic systems.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Annan medicinsk bioteknologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Other Medical Biotechnology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology (hsv//eng)

Nyckelord

Bacteria encapsulation
Chromosome analysis
Melting mapping
Nanochannels

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