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Sökning: onr:"swepub:oai:lup.lub.lu.se:d4716564-e0a1-45f3-b8f9-13c927ba566c" > Sample preparation ...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004150naa a2200409 4500
001oai:lup.lub.lu.se:d4716564-e0a1-45f3-b8f9-13c927ba566c
003SwePub
008181020s2012 | |||||||||||000 ||eng|
024a https://lup.lub.lu.se/record/d4716564-e0a1-45f3-b8f9-13c927ba566c2 URI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a kon2 swepub-publicationtype
100a Beech, Jason P.u Lund University,Lunds universitet,NanoLund,Annan verksamhet,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)ftf-job
2451 0a Sample preparation for single-cell whole chromosome analysis
264 1b Chemical and Biological Microsystems Society,c 2012
300 a 2 s.
520 a In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DNA in one piece and transferring it to a nano-channel for visualisation. In order to achieve control over the bacteria we encapsulate them in agarose, using flow focusing. The encapsulated bacteria can then be transported in microchannels to proximity with the nanochannels and then chemically lysis can be performed. Following lysis the intact genome can be extracted and transferred to the meandering nanochannel for analysis. We believe this device holds the potential to significantly decrease analysis times for single cell, whole genome analysis with the potential of opening up for automated, high-throughput genome analysis in microfluidic systems.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Annan medicinsk bioteknologi0 (SwePub)304992 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Other Medical Biotechnology0 (SwePub)304992 hsv//eng
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologi0 (SwePub)3042 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Medical Biotechnology0 (SwePub)3042 hsv//eng
653 a Bacteria encapsulation
653 a Chromosome analysis
653 a Melting mapping
653 a Nanochannels
700a Adolfsson, Karlu Lund University,Lunds universitet,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)ftf-kla
700a Holm, Stefanu Lund University,Lunds universitet,NanoLund,Annan verksamhet,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)ftf-sho
700a Yadegari, Farnazu Lund University4 aut
700a Freitag, Camillau University of Gothenburg4 aut
700a Fritzsche, Joachimu University of Gothenburg4 aut
700a Mir, Kalim U.u University of Oxford4 aut
700a Tegenfeldt, Jonas O.u University of Gothenburg,Lund University,Lunds universitet,NanoLund,Annan verksamhet,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)ftf-jte
710a NanoLundb Annan verksamhet4 org
773t Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012d : Chemical and Biological Microsystems Societyg , s. 998-999q <998-999z 9780979806452
8564 8u https://lup.lub.lu.se/record/d4716564-e0a1-45f3-b8f9-13c927ba566c

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