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Recovery of phenoty...
Recovery of phenotypes obtained by adaptive evolution through inverse metabolic engineering
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- Hong, Kuk-ki, 1976 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Nielsen, Jens B, 1962 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2012
- 2012
- Engelska.
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Ingår i: Applied and Environmental Microbiology. - 1098-5336 .- 0099-2240. ; 78:21, s. 7579-7586
- Relaterad länk:
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http://dx.doi.org/10...
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visa fler...
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In a previous study system level analysis of adaptively evolved yeast mutants showing improved galactose utilization revealed relevant mutations. The governing mutations were suggested to be in the Ras/PKA signaling pathway and ergosterol metabolism. Here site-directed mutants having one of the mutations, RAS2 Lys 77, RAS2 Tyr112 and ERG5 Pro 370 were constructed and evaluated. The mutants were also combined with over-expression of PGM2, earlier proved as a beneficial target for galactose utilization. The constructed strains were analyzed for their gross phenotype, transcriptome and targeted metabolites; and the results were compared to those obtained from reference strains and the evolved strains. The RAS2 Lys 77 mutation resulted in the highest specific galactose uptake rate among all the strains with an increased maximum specific growth rate on galactose. The RAS2 Tyr112 mutation also improved the specific galactose uptake rate and also resulted in many transcriptional changes, including ergosterol metabolism. The ERG5 Pro 370 mutation only showed a small improvement, but when it was combined with PGM2 over-expression, the phenotype was almost the same as that of the evolved mutants. Combination of the RAS2 mutations with PGM2 over-expression also showed a complete recovery of the adaptive phenotype in galactose utilization. Recovery of the gross phenotype by the reconstructed mutants was achieved with much fewer changes in the genome and transcriptome than for the evolved mutants. Our study demonstrates how the identification of specific mutations by systems biology can direct new metabolic engineering strategies for improving galactose utilization by yeast.
Ämnesord
- NATURVETENSKAP -- Biologi -- Evolutionsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Evolutionary Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
Nyckelord
- systems biology
- evolutionary engineering
- inverse metabolic engineering
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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