| 11. |
|
|
| 12. |
- Andersson, Helén, 1982-, et al.
(författare)
-
Low levels of the air pollutant 1-nitropyrene induce DNA damage, increased levels of reactive oxygen species and endoplasmic reticulum stress in human endothelial cells
- 2009
-
Ingår i: Toxicology. - : Elsevier BV. - 0300-483X .- 1879-3185. ; 262:1, s. 57-64
-
Tidskriftsartikel (refereegranskat)abstract
- Both epidemiological and experimental studies suggest that exposure to high levels of air pollution is a risk factor associated with cardiovascular disease. Traffic emission is a major source of exposure to persistent air pollutants such as nitrated polycyclic aromatic hydrocarbons (nitro-PAHs). 1-Nitropyrene (1-NP), one of the most abundant nitro-PAHs in diesel exhausts, was selected as a model nitro-PAH for the present study. The aim of the study was to investigate the effects of 1-NP in human umbilical vein endothelial cells (HUVECs) and the metabolic pathways involved. The nitroreductase inhibitor dicoumarol and the coplanar aryl hydrocarbon receptor (AhR) ligand PCB 126 were used to modulate the metabolism of 1-NP. The results revealed that low levels (< or =10microM) of 1-NP induced DNA damage, increased levels of reactive oxygen species (ROS) and increased protein expression of the endoplasmic reticulum (ER) stress chaperone GRP78. A decrease in cell viability was only observed following exposure to a higher level of 1-NP (15microM). Inhibition of nitroreductive metabolism by dicoumarol attenuated the induction of DNA damage, intracellular ROS levels and GRP78 expression. This suggests that the effects of 1-NP on HUVEC were mediated by metabolites mainly formed at nitroreduction. Our findings suggest that the human blood vessel endothelium is a sensitive target tissue for the major nitro-PAH constituent in diesel exhaust.
|
|
| 13. |
- Andersson, Helena M., et al.
(författare)
-
Activated protein C cofactor function of protein S: a critical role for Asp95 in the EGF1-like domain
- 2010
-
Ingår i: Blood. - : American Society of Hematology. - 1528-0020 .- 0006-4971. ; 115:23, s. 4878-4885
-
Tidskriftsartikel (refereegranskat)abstract
- Protein S has an established role in the protein C anticoagulant pathway, where it enhances the factor Va (FVa) and factor VIIIa (FVIIIa) inactivating property of activated protein C (APC). Despite its physiological role and clinical importance, the molecular basis of its action is not fully understood. To clarify the mechanism of the protein S interaction with APC, we have constructed and expressed a library of composite or point variants of human protein S, with residue substitutions introduced into the Gla, thrombin-sensitive region (TSR), epidermal growth factor 1 (EGF1), and EGF2 domains. Cofactor activity for APC was evaluated by calibrated automated thrombography (CAT) using protein S-deficient plasma. Of 27 variants tested initially, only one, protein S D95A (within the EGF1 domain), was largely devoid of functional APC cofactor activity. Protein S D95A was, however, gamma-carboxylated and bound phospholipids with an apparent dissociation constant (Kd(app)) similar to that of wildtype (WT) protein S. In a purified assay using FVa R506Q/R679Q, purified protein S D95A was shown to have greatly reduced ability to enhance APC-induced cleavage of FVa Arg306. It is concluded that residue Asp95 within EGF1 is critical forAPC cofactor function of protein S and could define a principal functional interaction site for APC. (Blood. 2010;115(23):4878-4885)
|
|
| 14. |
|
|
| 15. |
- Ekelund, Robin
(författare)
-
Connective Memory
- 2023
-
Ingår i: The Palgrave Encyclopedia of Memory Studies. - : Palgrave Macmillan. - 9783030937898
-
Bokkapitel (refereegranskat)abstract
- The concept of connective memory highlights how memories are shaped by connections between people, objects, media, and institutions. It derives from the growing discussions on how technological and digital developments affect contemporary memory culture and, in particular, from Andrew Hoskins’ conceptualization of a “connective turn” and a “new memory ecology.” Connective memory is both a methodological and an analytical tool as it inspires memory studies to explore memory by tracing and analyzing how different interactional trajectories intersect with and compete against each other. The concept is thusly designed to challenge ideas of “individual memories” and the binaries of individual and collective and active and passive. Even though the concept of connective memory is closely tied to technological and digital developments, it is important to note that it inspires memory research to investigate both online and offline connections. Connective memory has also been an influential concept in José van Dijck’s more wide-ranging conceptualization of a “culture of connectivity.” Her conceptualization not only engages with remembering but also deals with the connected society as a whole, and it provides a critical perspective on technology and social media platforms.
|
|
| 16. |
|
|
| 17. |
|
|
| 18. |
- Ekelund, Ulf, et al.
(författare)
-
Association of weight gain in infancy and early childhood with metabolic risk in young adults
- 2007
-
Ingår i: Journal of Clinical Endocrinology and Metabolism. - : The Endocrine Society. - 0021-972X .- 1945-7197. ; 92:1, s. 98-103
-
Tidskriftsartikel (refereegranskat)abstract
- CONTEXT: Early postnatal life has been suggested as an important window during which risks for long-term health may be influenced. OBJECTIVE: The aim of this study was to examine the independent associations between weight gain during infancy (0-6 months) and early childhood (3-6 yr) with components of the metabolic syndrome in young adults. DESIGN: This was a prospective cohort study (The Stockholm Weight Development Study). SETTING: The study was conducted in a general community. PARTICIPANTS: Subjects included 128 (54 males) singletons, followed from birth to 17 yr. MAIN OUTCOME MEASURE: None of these young adults met the full criteria for the metabolic syndrome. We therefore calculated a continuous clustered metabolic risk score by averaging the standardized values of the following components: waist circumference, blood pressure, fasting triglycerides, high-density lipoprotein cholesterol, glucose, and insulin level. RESULTS: Clustered metabolic risk at age 17 yr was predicted by weight gain during infancy (standardized beta = 0.16; P < 0.0001) but not during early childhood (standardized beta = 0.10; P = 0.23), adjusted for birth weight, gestational age, current height, maternal fat mass, and socioeconomic status at age 17 yr. Further adjustment for current fat mass and weight gain during childhood did not alter the significant association between infancy weight gain with the metabolic risk score (standardized beta = 0.20; P = 0.007). CONCLUSIONS: Rapid weight gain during infancy (0-6 months) but not during early childhood (3-6 yr) predicted clustered metabolic risk at age 17 yr. Early interventions to moderate rapid weight gain even at very young ages may help to reduce adult cardiovascular disease risks.
|
|
| 19. |
- Elias, S, et al.
(författare)
-
Gene Regulatory Network of Human GM-CSF-Secreting T Helper Cells
- 2021
-
Ingår i: Journal of immunology research. - : Hindawi Limited. - 2314-7156 .- 2314-8861. ; 2021, s. 8880585-
-
Tidskriftsartikel (refereegranskat)abstract
- GM-CSF produced by autoreactive CD4-positive T helper cells is involved in the pathogenesis of autoimmune diseases, such as multiple sclerosis. However, the molecular regulators that establish and maintain the features of GM-CSF-positive CD4 T cells are unknown. In order to identify these regulators, we isolated human GM-CSF-producing CD4 T cells from human peripheral blood by using a cytokine capture assay. We compared these cells to the corresponding GM-CSF-negative fraction, and furthermore, we studied naïve CD4 T cells, memory CD4 T cells, and bulk CD4 T cells from the same individuals as additional control cell populations. As a result, we provide a rich resource of integrated chromatin accessibility (ATAC-seq) and transcriptome (RNA-seq) data from these primary human CD4 T cell subsets and we show that the identified signatures are associated with human autoimmune diseases, especially multiple sclerosis. By combining information about mRNA expression, DNA accessibility, and predicted transcription factor binding, we reconstructed directed gene regulatory networks connecting transcription factors to their targets, which comprise putative key regulators of human GM-CSF-positive CD4 T cells as well as memory CD4 T cells. Our results suggest potential therapeutic targets to be investigated in the future in human autoimmune disease.
|
|
| 20. |
- Eriksson, Andreas, 1973
(författare)
-
Redesigning a Discipline-Specific Writing Assignment to Improve Writing on an EMI Programme of Engineering
- 2018
-
Ingår i: Journal of Academic Writing. - : Coventry University, Lanchester Library. - 2225-8973. ; 8:2, s. 48-66
-
Tidskriftsartikel (refereegranskat)abstract
- English-medium instruction (EMI) in higher education presents challenges at many different levels for educators and students. One of the challenges is disciplinary writing, as students typically study disciplinary content through, and also write in, English as a second or a foreign language. The present, exploratory intervention study uses the redesign of a writing assignment in a Master’s level engineering course at a Swedish university to investigate challenges of disciplinary writing in an EMI context. The study describes how collaboration between content and communication staff helped unpack some of the challenges that students faced. The results show that the students’ texts improved and that the redesign helped them to better adjust to a genre partially new to them. The study also underscores the value for programmes to have a clear plan for writing. The planning is likely to benefit from collaboration between disciplinary and communication faculty, as these participants bring different knowledge to the process.
|
|
