Endothelial PDGF-B retention is required for proper investment of pericytes in the microvessel wall.

• Several platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) family members display C-terminal protein motifs that confer retention of the secreted factors within the pericellular space. To address the role of PDGF-B retention in vivo, we deleted the retention motif by gene targeting in mice. This resulted in defective investment of pericytes in the microvessel wall and delayed formation of the renal glomerulus mesangium. Long-term effects of lack of PDGF-B retention included severe retinal deterioration, glomerulosclerosis, and proteinuria. We conclude that retention of PDGF-B in microvessels is essential for proper recruitment and organization of pericytes and for renal and retinal function in adult mice.

Keyword

Alleles

Amino Acid Motifs

Amino Acid Sequence

Animals

Cell Movement

Endothelial Growth Factors

metabolism

Endothelium

Vascular

metabolism

Glomerulosclerosis

Focal Segmental

genetics

Intercellular Signaling Peptides and Proteins

metabolism

Kidney

physiology

Lymphokines

metabolism

Mice

Microcirculation

metabolism

Microscopy

Fluorescence

Models

Genetic

Molecular Sequence Data

Mutation

Pericytes

metabolism

Phenotype

Platelet-Derived Growth Factor

metabolism

Protein Structure

Tertiary

Proteinuria

genetics

Proto-Oncogene Proteins c-sis

genetics

metabolism

Retina

metabolism

physiology

Retinal Degeneration

genetics

Vascular Endothelial Growth Factor A

Vascular Endothelial Growth Factors

Alleles

Publication and Content Type

ref (subject category)

art (subject category)