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Träfflista för sökning "db:Swepub ;spr:chi;conttype:(refereed);pers:(Xie Bin)"

Search: db:Swepub > Chinese > Peer-reviewed > Xie Bin

  • Result 1-5 of 5
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1.
  • Gao, Qin, et al. (author)
  • 甲基对硫磷水解酶分离条件的量热学优化
  • 2012
  • In: Guangzhou Chemical Industry and Technology. ; 40:8, s. 7-9
  • Journal article (peer-reviewed)abstract
    • Methyl parathion hydrolase ( MPH ) as and bovine serum albumin (BSA) as hybrid proteins, the target protein, bovine hemoglobin (BHb), myoglobin (Mb) enzyme thermal analysis was used to determine the best separation condition of methyl parathion hydrolase through the study of pH and the NaC1 concentration in mobile phase. The results showed that at pH 8.0, NaC1 concentration of 0.8 mol/L, the separation condition of methyl parathion hydrolase was the best which consistent with the condition reported in the literature.
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2.
  • Liang, Zhen-pu, et al. (author)
  • 单分子层修饰生物芯片基片的电化学研究
  • 2005
  • In: Journal of Wuhan University (Natural Science Edition). - 1671-8836. ; :2, s. 249-252
  • Journal article (peer-reviewed)abstract
    • A biotin-avidin modified biochip has been constructed, and its electrochemistry characteristics were studied with K4Fe(CN)(6).3H2O and ferrocene as the indicators by cyclic voltammograms. Electrochemical experiments showed that this biochip has high response signal, reproducibility,uniformity and low background signal; the ferrocene can be used as high efficiency electron medium. And these results were discussed in this paper.
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3.
  • Liu, Shichao, et al. (author)
  • 一种β-内酰胺类抗生素的酶热检测方法
  • 2019
  • In: Journal of Shanxi University (Natural Science Edition). ; :2021-02
  • Journal article (peer-reviewed)abstract
    • The penicillinase thermistor biosensor(Penicillinase sensor) was developed for the rapid monitoring of blood penem antibiotics concentration and rapid identification of extraneous penicillinase in milk on site.However, the wide application of the penicillinase thermistor biosensor was limited due to its intrinsic poor activity to hydrolyze cephem and carbapenem antibiotics.The recently identified carbapenemase New Delhi metallo-beta-lactamase 1(NDM-1) is able to hydrolyze all commercially available β-lactam antibiotics in high efficacy.We coupled the NDM-1 and the enzymatic thermistor biosensor to develop a NDM-1 thermistor biosensor(NDM-1 sensor) by the installment of the enzymatic thermistor with an enzyme column loaded with NDM-1 conjugated CPG beads.The NDM-1 sensor shows high response activity to Piperacillin(PIP),Ceftriaxone(CTRX), and Meropenem(MEM), and the response activity of the NDM-1 sensor to these three β-lactam antibiotics are all Zn2+ dependent.Moreover, the response activity of the NDM-1 sensor to Penicillin G(P), PIP, Cefazolin(CZO), CTRX, Cefepime(FEP) and MEM all linearly correlated with antibiotic concentration from 31.25 to 1 000 mg/L.Within pH from 6.0 to 8.0, the optimal response activity of the NDM-1 sensor to P,PIP, CZO, CTRX and FEP are found at pH 6.5, while the optimal response activity of the NDM-1 sensor to MEM is found at pH8.0.These data indicate that the featured activity of NDM-1 was well maintained after conjugation on CPG beads, and NDM-1 sensor is capable to quantitate three classes of β-lactam antibiotics including penem, cephem and carbapenem within a wide concentration range.
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4.
  • Liu, Yu-peng, et al. (author)
  • 应用酶热生物分析仪快速测量血糖的研究分析
  • 2013
  • In: Journal of Clinical and Experimental Medicine. ; 12:8, s. 579-581
  • Journal article (peer-reviewed)abstract
    • Objective To compare the enzyme thermistor bioanalysis equipment with blood glucose meter which is analyzed by automatic biochemical analyzer, validate the correct rate of this equipment. The long-term aim of the project was to develop a prototype for a bedside monitor system for semi-continuous monitoring of the blood glucose concentration. This was made possible by using the special advantage of the thermal sensor technique in combination with the adjustment of flow. Methods 150 patients were collected in our department. The glucose level was determined using a single channel enzyme thermistor device. Then the results were compared with lab biochemistry analysator. Results There was no significant difference between these two methods (P>0.05). The measuring method with single channel enzyme thermistor device is a reliable method in determining blood glucose level. Single channel enzyme thermistor keep a coincidence with automatic biochemical analyzer. The assay cycle time was 3 minutes. Comparative analysis between our device and the clinical system device showed an excellent correlation for 150 patient blood samples. The device was stabile for hundreds of injections over a period of 45 days. Conclusion Determined glucose in whole blood using a single channel Enzyme Thermistor device can be used to assess the severity of an illness and for evaluating therapeutic efficacy. The ability of the device to analyze whole blood without any pretreatment makes it possible to develop real-time analysis.
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5.
  • Wang, Xi, et al. (author)
  • 氨曲南竞争性抑制NDM-1对β-内酰胺类抗生素的水解
  • 2019
  • In: Journal of Shanxi University (Natural Science Edition). ; :2021-01
  • Journal article (peer-reviewed)abstract
    • We investigated the effect of Aztreonam on hydrolysis of β-lactam antibiotics by MBL using New Delhi Metallo-β-lactamase-1(NDM-1) as a model. The results showed that Aztreonam significantly inhibited hydrolysis of Nitrocefin and Meropenem by soluble NDM-1, but also inhibited hydrolysis of Penicillin G by CPG beads immobilized NDM-1(NDM-1 beads). Moreover, in spite of extensive washing for multiple times, the activity to hydrolyze Penicillin G by the Aztreoman pre-treated NDM-1 beads was just partially recovered. These data suggest that Aztreonam can covalently and stably bind on NDM-1, thus efficiently inhibiting hydrolysis of other kinds of β-lactam antibiotics by NDM-1 in a competitive way.
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  • Result 1-5 of 5
Type of publication
journal article (5)
Type of content
Author/Editor
Meng, Qinglai (2)
Li, Binchun (2)
Li, Shenwei (2)
Wu, Changxin (2)
Mecklenburg, Michael (1)
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Zhang, Sheng (1)
Wang, Xi (1)
Danielsson, Bengt (1)
Liu, Shuang (1)
Liu, Qing (1)
Gao, Qin (1)
Zhang, Jun-zhuo (1)
Xie, Wei-hong (1)
Liu, Shichao (1)
Liang, Zhen-pu (1)
Song, An-dong (1)
Liu, Yu-peng (1)
Tian, Zeng-min (1)
Li, Hong-yu (1)
Zhao, Si-yuan (1)
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University
Lund University (5)
Language
Research subject (UKÄ/SCB)
Natural sciences (5)

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