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- Frenzilli, G., et al.
(författare)
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DNA damage in eelpout (Zoarces viviparus) from Göteborg harbour
- 2004
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Ingår i: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. - : Elsevier BV. - 0027-5107. ; 552:1-2, s. 187-195
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Tidskriftsartikel (refereegranskat)abstract
- The relationship between DNA damage and the exposure of marine organisms to environmental contaminants was examined in the Göteborg harbour area. This research is part of a wider ecotoxicological study planned to evaluate the biological impact of chemical contamination in the River Göta estuary, following a bunker oil (10–100 tonnes) spill occurred in June 2003. Here we present data on the DNA strand breaks derived using the comet assay and the presence of apoptotic cells using the diffusion assay in nucleated erythrocytes of the eelpout (Zoarces viviparus) from the study area and at a clean reference site. Polycyclic aromatic hydrocarbon metabolites were also analyzed in the bile of exposed fish. The results showed a high level of damaged DNA, paralleled by a peak in bile PAH metabolites, in fish from the most impacted site, 3 weeks after the oil spill. A significant recovery was observed in specimens from the spill site, 5 months later, but not in fish caught in the middle part of Göteborg harbour, which is chronically subjected to heavy chemical pollution. The levels of apoptic cells did not show any marked variations, but a significant recovery was observed in fish from the oil impacted site 5 months after the spill
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- Förlin, Lars, 1950, et al.
(författare)
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A tiered test system for fish toxicity
- 2004
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Ingår i: Better chemicals control within Reach. - 9172837047 ; , s. 109-117
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 8. |
- Förlin, Lars, 1950, et al.
(författare)
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Epidemiological studies indicate a cause-effect relationship between pulp mill effluent exposure and male-biased sex ratios in fish.
- 2004
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Ingår i: Platform presentation at the 5th International Conference on Fate and Effects of Pulp and Paper mill Effluents. Seattle, Washington, June 1-4, 2003. Extended abstract published in: Pulp and Paper Mill Effluent Environmental Fate and Effects Conference Proceedings. Ed. D. Borton, T.J. Hall, R.P. Fischer and J.F. Fischer. DEStech Publications, Inc., Lancaster. - Lancaster : DEStech Publ, Inc. ; , s. 48-54, s. 48-54
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Konferensbidrag (refereegranskat)
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| 9. |
- Larsson, D. G. Joakim, 1969, et al.
(författare)
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Male-biased sex ratios of fish embryos near a pulp mill: temporary recovery after a short-term shutdown.
- 2002
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Ingår i: Environmental health perspectives. - 0091-6765. ; 110:8, s. 739-42
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Tidskriftsartikel (refereegranskat)abstract
- In a previous study we showed that broods from the viviparous eelpout Zoarces viviparus were significantly male biased in 1998 in the vicinity of a large kraft pulp mill on the Swedish Baltic coast. One suggested hypothesis was that masculinizing compounds in the effluent were affecting gonadal differentiation of the embryos, resulting in skewed sex ratios. In this article, we present further evidence for a causal relationship between the exposure to the effluent and the male-biased sex ratios. Analyses of historical samples showed that the eelpout produced male-biased broods close to the mill in 1997 in addition to 1998. During 1999, the mill was shut down for 17 days, coinciding with the period when the gonads of the eelpout embryos differentiate. Subsequently, in the fall of 1999, the sex ratios were no longer male biased; however, the following year (2000), a significant male bias reappeared. Investigations at 13 sites for up to 4 years showed a relatively stable sex ratio around 50/50, with the exceptions by the mill and with few observations of deviating ratios at other sites. Several reports document endocrine disturbances in fish near pulp and paper mills, including the expression of male secondary sex characters in female fish. The repeatedly identified male bias at the investigated mill, the normalization after mill shutdown, and the reappearance the following year indicate that pulp mill effluents also can affect sex ratios of nearby fish.
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- Larsson, D. G. Joakim, 1969, et al.
(författare)
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More male fish embryos near a pulp mill
- 2000
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Ingår i: Environmental Toxicology and Chemistry. - : Wiley. - 0730-7268 .- 1552-8618. ; 19:12, s. 2911-2917
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Tidskriftsartikel (refereegranskat)abstract
- Exposure to endocrine disrupters during early life stages may cause severe long-term effects. One process likely to be affected is sexual differentiation, which in fish can be influenced by both exogenous androgens and estrogens. To investigate if sexual differentiation is disturbed in wild fish exposed to pollutants, we studied embryonic sex ratios in a viviparous, gonochoristic fish species, the eelpout (Zoarces viviparus), whose embryos complete sexual differentiation before birth. The normal sex ratio of the eelpout was close to 50% females at four reference stations. In the effluent gradient of a large Swedish pulp mill, however, the sex ratios were significantly male biased. In light of the endocrine disturbances found near several pulp and paper mills in Europe and North America, it is suggested that endocrine disrupters may interfere with sexual differentiation and, thereby, affect the reproductive potential of fish populations.
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