| 181. |
- Larsson, Nirina, et al.
(författare)
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Lipid mediator profiles differ between lung compartments in asthmatic and healthy humans
- 2014
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Ingår i: European Respiratory Journal. - : European Respiratory Society (ERS). - 0903-1936 .- 1399-3003. ; 43:2, s. 453-463
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Tidskriftsartikel (refereegranskat)abstract
- Oxylipins are oxidised fatty acids that can exert lipid mediator functions in inflammation, and several oxylipins derived from arachidonic acid are linked to asthma. This study quantified oxylipin profiles in different regions of the lung to obtain a broad-scale characterisation of the allergic asthmatic inflammation in relation to healthy individuals. Bronchoalveolar lavage fluid (BALF), bronchial wash fluid and endobronchial mucosal biopsies were collected from 16 healthy and 16 mildly allergic asthmatic individuals. Inflammatory cell counts, immunohistochemical staining and oxylipin profiling were performed. Univariate and multivariate statistics were employed to evaluate compartment-dependent and diagnosis-dependent oxylipin profiles in relation to other measured parameters. Multivariate modelling showed significantly different bronchial wash fluid and BALF oxylipin profiles in both groups ((RY)-Y-2[cum]=0.822 and Q(2)[cum]=0.759). Total oxylipin concentrations and five individual oxylipins, primarily from the lipoxygenase (LOX) pathway of arachidonic and linoleic acid, were elevated in bronchial wash fluid from asthmatics compared to that from healthy controls, supported by immunohistochemical staining of 15-LOX-1 in the bronchial epithelium. No difference between the groups was found among BALF oxylipins. In conclusion, bronchial wash fluid and BALF contain distinct oxylipin profiles, which may have ramifications for the study of respiratory diseases. Specific protocols for sampling proximal and distal airways separately should be employed for lipid mediator studies.
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| 182. |
- Le Febvrier, Arnaud, et al.
(författare)
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An upgraded ultra-high vacuum magnetron-sputtering system for high-versatility and software-controlled deposition
- 2021
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Ingår i: Vacuum. - : Pergamon-Elsevier Science Ltd. - 0042-207X .- 1879-2715. ; 187
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Tidskriftsartikel (refereegranskat)abstract
- Magnetron sputtering is a widely used physical vapor deposition technique. Reactive sputtering is used for the deposition of, e.g, oxides, nitrides and carbides. In fundamental research, versatility is essential when designing or upgrading a deposition chamber. Furthermore, automated deposition systems are the norm in industrial production, but relatively uncommon in laboratory-scale systems used primarily for fundamental research. Combining automatization and computerized control with the required versatility for fundamental research constitutes a challenge in designing, developing, and upgrading laboratory deposition systems. The present article provides a detailed description of the design of a lab-scale deposition chamber for magnetron sputtering used for the deposition of metallic, oxide, nitride and oxynitride films with automated controls, dc or pulsed bias, and combined with a coil to enhance the plasma density near the substrate. LabVIEW software (provided as Supplementary Information) has been developed for a high degree of computerized or automated control of hardware and processes control and logging of process details.
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| 183. |
- Lefaudeux, Diane, et al.
(författare)
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U-BIOPRED clinical adult asthma clusters linked to a subset of sputum omics
- 2017
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Ingår i: Journal of Allergy and Clinical Immunology. - : Elsevier BV. - 0091-6749 .- 1097-6825. ; 139:6, s. 1797-1807
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Asthma is a heterogeneous disease in which there is a differential response to asthma treatments. This heterogeneity needs to be evaluated so that a personalized management approach can be provided.OBJECTIVES: We stratified patients with moderate-to-severe asthma based on clinicophysiologic parameters and performed an omics analysis of sputum.METHODS: Partition-around-medoids clustering was applied to a training set of 266 asthmatic participants from the European Unbiased Biomarkers for the Prediction of Respiratory Diseases Outcomes (U-BIOPRED) adult cohort using 8 prespecified clinic-physiologic variables. This was repeated in a separate validation set of 152 asthmatic patients. The clusters were compared based on sputum proteomics and transcriptomics data.RESULTS: Four reproducible and stable clusters of asthmatic patients were identified. The training set cluster T1 consists of patients with well-controlled moderate-to-severe asthma, whereas cluster T2 is a group of patients with late-onset severe asthma with a history of smoking and chronic airflow obstruction. Cluster T3 is similar to cluster T2 in terms of chronic airflow obstruction but is composed of nonsmokers. Cluster T4 is predominantly composed of obese female patients with uncontrolled severe asthma with increased exacerbations but with normal lung function. The validation set exhibited similar clusters, demonstrating reproducibility of the classification. There were significant differences in sputum proteomics and transcriptomics between the clusters. The severe asthma clusters (T2, T3, and T4) had higher sputum eosinophilia than cluster T1, with no differences in sputum neutrophil counts and exhaled nitric oxide and serum IgE levels.CONCLUSION: Clustering based on clinicophysiologic parameters yielded 4 stable and reproducible clusters that associate with different pathobiological pathways.
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| 184. |
- Lindahl, Karin Beland, et al.
(författare)
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The Swedish forestry model : more of everything?
- 2017
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Ingår i: Forest Policy and Economics. - : Elsevier. - 1389-9341 .- 1872-7050. ; 77, s. 44-55
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Tidskriftsartikel (refereegranskat)abstract
- "The Swedish forestry model" refers to the forest regime that evolved following the 1993 revision of the Swedish Forestry Act. It is key to Swedish forest politics and used to capture the essence of a sustainable way of managing forests. However, the ideas, institutions and practices comprising the model have not been comprehensively analyzed previously. Addressing this knowledge gap, we use frame analysis and a Pathways approach to investigate the underlying governance model, focusing on the way policy problems are addressed, goals, implementation procedures, outcomes and the resulting pathways to sustainability. We suggest that the institutionally embedded response to pressing sustainability challenges and increasing demands is expansion, inclusion and integration: more of everything. The more-of-everything pathway is influenced by ideas of ecological modernization and the optimistic view that existing resources can be increased. Our findings suggest that in effect it prioritizes the economic dimension of sustainability. While broadening out policy formulation it closes down the range of alternative outputs, a shortcoming that hampers its capacity to respond to current sustainability challenges. Consequently, there is a need for a broad public debate regarding not only the role of forests in future society, but also the operationalization of sustainable development.
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| 185. |
- Lindberg, Anne, et al.
(författare)
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Seven-year cumulative incidence of COPD in an age-stratified general population sample.
- 2006
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Ingår i: Chest. - : Elsevier BV. - 0012-3692 .- 1931-3543. ; 129:4, s. 879-885
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Tidskriftsartikel (refereegranskat)abstract
- AIM: To estimate the cumulative incidence of COPD and risk factors related to the development of COPD, including evaluation of the relationship between Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage 0 (ie, respiratory symptoms and normal lung function) and the development of COPD, in an age-stratified general population sample of middle-aged and elderly individuals. METHOD: The third survey of the Obstructive Lung Disease in Northern Sweden studies cohort I (three age strata born in 1919 to 1920, 1934 to 1935, and 1949 to 1950) was performed in 1996, and 5,189 subjects (88%) responded to the postal questionnaire. Of the responders, a random sample (1,500 subjects) was invited to an examination in 1996 and in 2003. A total of 963 subjects performed spirometry on both occasions. COPD was defined according to the spirometric criteria of the GOLD. Two levels of disease severity, grade I and higher (GOLD criteria, FEV(1)/FVC ratio of < 0.70) and also grade II and higher (GOLD II criteria, FEV(1)/FVC ratio of < 0.70 and FEV(1) <80% predicted). RESULTS: The 7-year cumulative incidence of COPD was 11.0% and 4.9%, respectively, according to GOLD and GOLD II, and was significantly related to smoking (smokers, 18.8% and 10.6%, respectively; ex-smokers, 10.5% and 5.2%, respectively; non-smokers, 7.6% and 1.6%, respectively). Incident COPD according to GOLD, but not according to GOLD II, was significantly associated with increasing age. Most respiratory symptoms at study entry were markers of increased risk for incident COPD when analyzed in a multivariate model adjusting for confounders. CONCLUSION: The GOLD criteria yielded a higher cumulative incidence (11.0%) compared to the GOLD II (4.9%). Smoking, but not gender, was associated with incident COPD. Most respiratory symptoms at the beginning of the observation period marked an increased risk for developing COPD, thus the classification GOLD stage 0 seems relevant among middle-aged and elderly persons.
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| 186. |
- Lindgren, M., et al.
(författare)
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Applicability of using internal GPGPUs in industrial control systems
- 2014
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Ingår i: 19th IEEE International Conference on Emerging Technologies and Factory Automation, ETFA 2014. - 9781479948468 ; , s. Article number 7005096-
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Konferensbidrag (refereegranskat)abstract
- Industrial control systems are continuously increasing in functionality, connectivity, and levels of integration, and as a consequence they require more computational power. At the same time, these systems have specific requirements related to cost, reliability, timeliness, and thermal power dissipation, which put restrictions on the hardware and software used. Today the high-end embedded CPUs not only provide multiple cores, but also integrated graphics processors (GPU) at close to no additional cost. The use of GPUs for general processing have several potential values in industrial control systems; 1) the added computational power and the high parallelism could pave way for new functionality and 2) the integrated GPU could potentially replace other hardware and thereby reduce the overall cost. In this paper we investigate the applicability of using integrated GPUs in industrial control systems. We do this by evaluating the performance of GPUs with respect to computational problem types and sizes typically found in industrial control systems. In the end we conclude that GPUs are no obvious match for industrial control systems and that several hurdles remain before a wide adoption can be motivated.
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| 187. |
- Liu, Meng, et al.
(författare)
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On providing real-time guarantees in cloud-based platforms
- 2016
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Ingår i: IEEE International Workshop on Factory Communication Systems - Proceedings, WFCS. - 9781509023394
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Konferensbidrag (refereegranskat)abstract
- Cloud technologies are gaining more and more attentions in recent years. Cloud-based service brings benefits in cost, energy efficiency, sharing of resources, increased flexibility, adaptability and evolvability. However, there are a number of associated challenges that need to be properly addressed before applying the cloud technique generally in industries. Providing efficient and predictable computation and communication is one of the important challenges, since many industrial systems (e.g. a control system) have specific timing requirements. Our current work thus focuses on guaranteeing the predictability of a cloud-based service. Virtualization, as one of the key technologies in Cloud Computing, is used to abstract details of resources away from end-services which simplifies the resource sharing. It thus improves the resource utilization and saves budget for end-users. In this preliminary work, we have implemented a distributed system using virtualization techniques (including virtual machines and virtual switches). Additionally, we generate a number of experiments to investigate how QoS policies can help us to provide real-time communication guarantees.
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| 188. |
- Lucking, Andrew J, et al.
(författare)
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Diesel exhaust inhalation increases thrombus formation in man
- 2008
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Ingår i: European Heart Journal. - : Oxford University Press (OUP). - 0195-668X .- 1522-9645. ; 29:24, s. 3043-3051
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Tidskriftsartikel (refereegranskat)abstract
- AIMS: Although the mechanism is unclear, exposure to traffic-derived air pollution is a trigger for acute myocardial infarction (MI). The aim of this study is to investigate the effect of diesel exhaust inhalation on platelet activation and thrombus formation in men. METHODS AND RESULTS: In a double-blind randomized crossover study, 20 healthy volunteers were exposed to dilute diesel exhaust (350 microg/m(3)) and filtered air. Thrombus formation, coagulation, platelet activation, and inflammatory markers were measured at 2 and 6 h following exposure. Thrombus formation was measured using the Badimon ex vivo perfusion chamber. Platelet activation was assessed by flow cytometry. Compared with filtered air, diesel exhaust inhalation increased thrombus formation under low- and high-shear conditions by 24% [change in thrombus area 2229 microm(2), 95% confidence interval (CI) 1143-3315 microm(2), P = 0.0002] and 19% (change in thrombus area 2451 microm(2), 95% CI 1190-3712 microm(2), P = 0.0005), respectively. This increased thrombogenicity was seen at 2 and 6 h, using two different diesel engines and fuels. Diesel exhaust also increased platelet-neutrophil and platelet-monocyte aggregates by 52% (absolute change 6%, 95% CI 2-10%, P = 0.01) and 30% (absolute change 3%, 95% CI 0.2-7%, P = 0.03), respectively, at 2 h following exposure compared with filtered air. CONCLUSION: Inhalation of diesel exhaust increases ex vivo thrombus formation and causes in vivo platelet activation in man. These findings provide a potential mechanism linking exposure to combustion-derived air pollution with the triggering of acute MI.
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| 189. |
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| 190. |
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