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- Göransson, Anna, 1970, et al.
(author)
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An in vitro comparison of possibly bioactive titanium implant surfaces.
- 2009
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In: Journal of Biomedical Materials Research Part A. - : Wiley. - 1552-4965 .- 1549-3296. ; 88:4, s. 1037-1047
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Journal article (peer-reviewed)abstract
- The aim of the study was to compare Ca and P formation (CaP) and subsequent bone cell response of a blasted and four different possibly bioactive commercially pure (cp) titanium surfaces; 1. Fluoride etched (Fluoride), 2. Alkali-heat treated (AH), 3. Magnesium ion incorporated anodized (TiMgO), and 4. Nano HA coated and heat treated (nano HA) in vitro. Furthermore, to evaluate the significance of the SBF formed CaP coat on bone cell response. The surfaces were characterized by Optical Interferometry, Scanning Electron Microscopy (SEM) and X-ray Photoelectron Spectroscopy (XPS). CaP formation was evaluated after 12, 24 and 72 h in simulated body fluid (SBF). Primary human mandibular osteoblast-like cells were cultured on the various surfaces subjected to SBF for 72 h. Cellular attachment, differentiation (osteocalcin) and protein production (TGF-beta(1)) was evaluated after 3 h and 10 days respectively. Despite different morphological appearances, the roughness of the differently modified surfaces was similar. The possibly bioactive surfaces gave rise to an earlier CaP formation than the blasted surface, however, after 72 h the blasted surface demonstrated increased CaP formation compared to the possibly bioactive surfaces. Subsequent bone cell attachment was correlated to neither surface roughness nor the amount of formed CaP after SBF treatment. In contrast, osteocalcin and TGF-beta(1) production were largely correlated to the amount of CaP formed on the surfaces. However, bone response (cell attachment, osteocalcin and TGF-F production) on the blasted controls were similar or increased compared to the SBF treated fluoridated, AH and TiMgO surface. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2008.
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| 2. |
- Göransson, Anna, 1970, et al.
(author)
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Bone formation at titanium implants prepared with iso- and anisotropic surfaces of similar roughness: an in vivo study.
- 2005
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In: Clinical implant dentistry and related research. - 1523-0899. ; 7:1, s. 17-23
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Journal article (peer-reviewed)abstract
- BACKGROUND: Implant surface topography influences the bone response after implantation. However, the importance of surface orientation is not known. Purpose: The aim of this study was to investigate the bone tissue response and the stability of titanium implants prepared with isotropic and anisotropic surfaces of similar roughness. MATERIALS AND METHODS: A total of 18 implants were divided into two groups and were inserted into the femurs of nine rabbits for 12 weeks. Confocal laser scanning microscopy was used for the topographic description to verify that the two different surfaces were modified as intended. The stability of the implants was recorded by resonance frequency (RF) measurements at insertion and at time of removal, after which the implants were evaluated histomorphometrically. RESULTS: RF measurements showed that implant stability increased with time. However, there was no significant difference between the two different surface modifications at insertion and after 12 weeks. The histomorphometric comparison revealed no statistically significant differences in regard to either bone-to-metal contact or bone area inside the threads. CONCLUSION: Titanium implants prepared with isotropic and anisotropic surfaces of similar roughness integrate similarly to bone during the 3 months after implantation.
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| 3. |
- Göransson, Anna, 1970, et al.
(author)
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Inflammatory response to oxidised surface with Mg 2+ -ions incorporated in vitro
- 2004
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In: 7th World Biomaterials Congress, Sidney, 17 -21 May 2004.
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Conference paper (peer-reviewed)abstract
- Introduction Oxide films that grow spontaneously on titanium surfaces in contact with air may explain the bio-passivity of the material. Various procedures have been carried out to modify the properties of titanium oxide films to further improve the biocompatibility. Anodic oxidation is one technique to increase the thickness of the oxide layer that demonstrates significant stronger bone response in vivo. The concomitant increase in surface roughness and size and presence of pores of the thicker oxide layer seems to work as a potential contributor to the results (1). Attempts to implant ion in the oxide layers to overcome the drawbacks of calcium phosphate coatings (hydroxylapatite) such as i.e. delaminating and biodegradation during function seem promising (2). However the reasons why a thicker oxide layer with and without incorporated ions is favourable compared to conventionally turned and blasted surfaces are not fully understood. The aim of this study was to compare the early inflammatory response to the turned, blasted and electrochemically oxidised surface with Mg 2+ ions incorporated. Materials and Methods A total of 108 pure titanium discs were prepared with a turned surface. Thirty-six were kept as turned controls while 36 were blasted with 75 μm Al2O3 particles and 36 underwent electrochemically oxidation and Mg 2+ ion incorporation. MicroXam™, (Phase-Shift, Tucson, Arizona, USA) was used to for topographical characterisation. The disks were incubated with human mononuclear cells isolated from buffy coats of healthy blood donors (C-lab, Blood Supply Unit Sahlgrenska University hospital, Sweden) and cultured at a concentration of 106 cells/ml in 24 well cell culture plates. Half of the discs with the different treated surfaces were immediately treated with LPS while half were left without any stimuli. The incubation times were 24 and 72h. After each incubation period the incubation medium was collected and centrifuged. The supernatant was analysed with respect to cell viability and cytokine levels. Cell viability was estimated by analysing the content of lactatdehydrogenas (LDH)(Sahlgrenska University hospital, C-lab) and a commercially available ELISA assay (Biotrak system™, Amersham Bioscience, UK) was used to quantify TNF-α and IL -10 levels. The cells adherent to the material was stained with 2,6- diamidino-2-phenyindole (DAPI) (Sigma, USA) to evaluate the total cell number. In order to characterize differentiation of the adherent cells expression of 27E10 and RM3/1 (Biogenisis, UK) was used. The marker 27E10 and RM3/1 define acute and chronic inflammatory phenotypes respectively. Differentiated cells were evaluated as the percentage of positively stained cells from the total cell numbers. Results Surface evaluation revealed similar roughness for the turned control and the anodised surface with Mg 2+ ions incorporated while the blasted surface demonstrated a rougher surface profile (fig 1, 2). Fig 1 Fig 2 Sa-average height deviation (ym) SURFACE CTR Blasted Anodised+Mg Mean SA 1,2 1,0 ,8 ,6 ,4 ,2 0,0 Sdr-developed surface area (%) SURFACE CTR Blasted Anodised+Mg Mean SDR 40 30 20 10 0 LDH values were generally low for all surfaces (within the range of 0.8-1.6 μkat/l) but were slightly increased after LPS stimulation and after 72h. TNF-α was transient higher day one and after LPS stimulation especially on the turned control surface (fig 3, 4) Fig 3 Fig 4 TNF-a 24h (pg/ml) SURFACE CTR Blasted Anodised+Mg Mean C 3000 2000 1000 0 LPS LPSLPS+ TNF-a 72h (pg/ml) SURFACE CTR Blasted Anodised+Mg Mean C 400 300 200 100 0 LPS LPSLPS+ IL-10 levels were generally low irrespective of time. Increased IL-10 amounts after LPS stimulation and after 24 h were observed for all surfaces. The total cell numbers decreased on all surfaces from 24h to 72h but there were no major difference between stimulated and un-stimulated wells. Acute monocytic phenotype 27E10 marker dominated on all surfaces while the expression of the chronic RM3/1 marker was almost absent on all surfaces both at 24 and 72h. Conclusion The present study indicates a surface topography- and chemistry related difference in the acute inflammatory response with a stronger acute inflammatory response to the turned control compared to the blasted and anodised surface with Mg 2+ ions incorporated. References 1.Göransson, A, Jansson, E, Tengvall, P, Wennerberg, A. Bone formation after 4 weeks …topography : an in vivo study. Biomaterials 2002; 24: 197-205 2.Sul YT. PhD Thesis 2002, Göteborg University, Sweden
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| 4. |
- Göransson, Anna, 1970, et al.
(author)
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Inflammatory response to titanium surfaces with fibrinogen and catalase coatings: an in vitro study.
- 2007
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In: Journal of biomedical materials research. Part A. - : Wiley. - 1549-3296 .- 1552-4965. ; 80:3, s. 693-9
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Journal article (peer-reviewed)abstract
- The aim of the present study was to evaluate the possibility to modulate the early inflammatory response in vitro by coating titanium surfaces with candidate proinflammatory (fibrinogen coated turned titanium "Fib") and antiinflammatory proteins (catalase on top of fibrinogen coated turned titanium "Cat"). Additionally, turned titanium surfaces (Ti) were used as controls. The discs were incubated with human mononuclear cells. Adhered cells were investigated with respect to number, viability, differentiation (acute marker 27E10 vs. chronic marker RM3/1), and cytokine production (TNF-alpha and IL-10), after 24 and 72 h. The results indicated that it is possible to modulate the inflammatory response with protein coatings. However, the strongest inflammatory response, indicated by increased number of adhered cells and release of pro and antiinflammatory mediators, was induced by Cat. Furthermore, the cytokine production on this surface was not sensitive to LPS stimulation. Differentiation measured as the expression of the chronic cell surface marker, dominated after 72 h for all surface modifications and Cat displayed an increased number compared to the others. A decrease in the total number of adhered cells and amounts of TNF-alpha were observed on all surfaces over time. The cell viability was, in general, high for all tested surfaces. In conclusion, the study proved it possible to influence the early inflammatory response in vitro by immobilizing protein coatings to titanium surfaces. However, the catalase surface demonstrated the strongest inflammatory response, and the possibility to selectively use the potent antiinflammatory capacity of catalase needs to be further evaluated.
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| 5. |
- Göransson, Anna, 1970, et al.
(author)
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Inflammatory response to titanium surfaces with with Potential Bioactive Properties: An In Vitro Study
- 2006
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In: Clinical Implant Dentistry and Related Research. ; 8:4, s. 210-217
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Journal article (peer-reviewed)abstract
- Background: The current hard tissue implants research aims to accelerate bone healing by designing surfaces that are bioactive. However, the role of the inflammatory response to these surfaces is so far incompletely described. Purpose: The aim of the study was to evaluate early inflammatory response in vitro to a potentially bioactive surface—an anodized surface with Mg ions incorporated (anodized/Mg)—and to compare it to a turned, a blasted, and an anodized surface. Materials and Methods: An interferometer was used for topographical characterizations. The disks were incubated with human mononuclear cells. Adherent cells were investigated with respect to number of cells, viability, differentiation, and cytokine production with and without lipopolysaccharide stimulation after 24 and 72 hours. Results: The number of adhered mononuclear cells differed significantly between the different modified surfaces, with the highest number on the anodized surface. However, there were no significant differences in cytokine production and differentiation between the different modified surfaces. The amount of anti-inflammatory mediator interleukin-10 remained over time, while the number of cells and pro-inflammatory cytokine tumor necrosis factor-α decreased. The cells were viable on all surfaces, respectively. Conclusion: The anodized surfaces with and without Mg ions showed an increased cell adherence, however, otherwise an inflammatory response similar to the turned and blasted surfaces. Furthermore, the potentially bioactive anodized/Mg surface showed a similar response to the TiUnite-like anodized surface despite the former having a surface roughness of a smoother character.
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