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Numrering	Referens	Omslagsbild	Hitta
4941.	Wirtberg, I, et al. (författare) Marte Meo and collaboration meetings. The development of a school-based intervention - An open trial 2004 Ingår i: 5th European Congress of Family Therapy and Systemic Practice in Berlin 27 aug - 2 oct. Konferensbidrag (refereegranskat)
4942.	Witon, Ewa, et al. (författare) Hippodonta subcostulata (Hustedt) Lange-Bertalot, Metzeltin et Witkowski and some fragilarioid diatom taxa from the Holocene lacustrine sediments of the Faeroe Islands 2004 Ingår i: Diatom Research. - 0269-249X. ; 19:1, s. 123-134 Tidskriftsartikel (refereegranskat)
4943.	Witon, Ewa (författare) Holocene Marine and Freshwater Diatoms from the Faeroe Island. Taxonomy and environmental influences 2004 Doktorsavhandling (övrigt vetenskapligt/konstnärligt)
4944.	Witon, Ewa (författare) Holocene marine and freshwater diatoms from the Faeroe islands 2004 Doktorsavhandling (övrigt vetenskapligt/konstnärligt)
4945.	Woestenenk, Esmeralda A., et al. (författare) His tag effect on solubility of human proteins produced in Escherichia coli: a comparison between four expression vectors. 2004 Ingår i: Journal of structural and functional genomics. - 1345-711X .- 1570-0267. ; 5:3, s. 217-29 Tidskriftsartikel (refereegranskat)abstract We have compared four different vectors for expression of proteins with N- or C-terminal hexahistidine (His6) tags in Escherichia coli by testing these on 20 human proteins. We looked at a total recombinant protein production levels per gram dry cell weight, solubility of the target proteins, and yield of soluble and total protein when purified by immobilized metal ion affinity purification. It was found that, in general, both N- and C-terminal His6 tags have a noticeable negative affect on protein solubility, but the effect is target protein specific. A solubilizing fusion tag was able to partly counteract this negative effect. Most target proteins could be purified under denaturing conditions and about half of the proteins could be purified under physiological conditions. The highest protein production levels and yield of purified protein were obtained from a construct with C-terminal His tag. We also observe a large variation in cell growth rate, which we determined to be partly caused by the expression vectors and partly by the targets. This variation was found to be independent of the production level, solubility and tertiary structure content of the target proteins.
4946.	Wolff, Helene, 1956 (författare) Studies of chronic ulcers and larval therapy 2004 Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract Our aims in this study were to learn more about chronic ulcer pathogenesis (Paper I) and larval therapy (Paper II-V).Materials and methods: Paper I: 26 bacterial isolates of P. aeruginosa from chronic ulcers were examined by PCR technique for detection of the genes of two virulence factors (elastase and alkaline proteinase). Zymography (Paper I & Paper III)) and azocasein assay (Paper I) were used for detection of excreted proteases. Paper II: The debridemental effect of the larvae of Lucilia sericata was investigated in 74 necrotic or sloughy ulcers. Cleaned ulcer area was estimated as thirds of the total area and documented photographically. Adverse side-effects were registered as yes or no - answers and pain was measured with visual analogue scale of pain. Paper III: Larval secretions were collected after the larvae had been emerged in different growth media, in phosphate buffered saline and in a wound. Paper IV: Bacterial cultures were taken before and after larval therapy from a leg ulcer colonised by methicillin-resistant Staphylococcus aureus. Paper V: Disposable equipment and mainly aseptically produced food were used for rearing fly larvae. The humidity (40%) and temperature (25oC) were kept at a constant level. The fly eggs were disinfected with chloramine solution 0.25%. Fifteen cultures were taken from disinfected larvae and single ones were taken from meat; fly drinking water and from non-disinfected larvae, under aerobic and anaerobic conditions. In the routine disinfection control disinfected eggs were streaked over a horse blood agar plate (Oxoid), which was incubated at 37oC in a thermostat for 24h under aerobic conditions. Results: Paper I: Both proteinase genes were detected in all the samples. The level of proteinase expression differed between the isolates, but was stable for each strain from time to time. Paper II: Some 86% of the wounds were well debrided. A single application of larvae for two or three days was sufficient for debridement in two-thirds of the ulcer cases, but in cases with a thick eschar two applications were required. The larvae seemed to thrive especially well in the wounds of diabetic patients. Most patients (41%) felt no difference in pain during larval therapy, while a quarter felt less and, although 34% felt an increase in pain, they generally wanted to continue the therapy. None of the patients experienced a tickling sensation or bleeding. Odour was mostly reduced or un-changed. Paper III: The larvae excreted predominantly serine proteases. Paper IV: MRSA was cleansed from the ulcer. Paper V: The larvae developed satisfactorily. Non-pathogenic spore-bearing bacteria were regularly detected on the larvae after disinfection. The disinfection control was sufficient for detecting the usual wound pathogens. Conclusions: Paper I: The bacterial phenotype should be taken into account in future leg ulcer studies. Paper II: Larval therapy is an effective and well tolerated debridemental therapy. Paper III: The larvae excrete proteases for their extra-corporal digestion. Paper IV: Larvae have an antibacterial effect. Paper V: The rearing technique is easy, reliable, safe and odour-free.
4947.	Wolff, Ulrika, 1956 (författare) The sixth BDA interantional conference. Dyslexi aktuellt om läs- och skrivsvårigheter, 3, 16-17. 2004 Rapport (övrigt vetenskapligt/konstnärligt)
4948.	Wolter, Stefan, 2005, et al. (författare) OECD Examiners’ Report on Educational Research and Development in England 2004 Ingår i: European Educational Research Journal. - 1474-9041. ; 3:2, s. 510-526 Tidskriftsartikel (refereegranskat)
4949.	Wolzt, Michael, et al. (författare) Effect of recombinant factor Vlla on melagatran-induced inhibition of thrombin generation and platelet activation in healthy volunteers 2004 Ingår i: THROMBOSIS AND HAEMOSTASIS. - 0340-6245. ; 91:6, s. 1090-1096 Tidskriftsartikel (refereegranskat)
4950.	Wosinska-Becler, Katarzyna, 1972, et al. (författare) Cytokine production in peripheral blood cells during and outside the pollen season in birch-allergic patients and non-allergic controls 2004 Ingår i: Clin Exp Allergy. ; 34:1, s. 123-30. Tidskriftsartikel (refereegranskat)abstract BACKGROUND : The naturally occurring pollen season permits observation of the kinetic changes in the process of allergic inflammation. We examined cytokine production in peripheral blood (PB) T cells and monocytes obtained from birch-allergic patients both during and outside the pollen season. METHODS : PB from 16 patients and six healthy controls was obtained during the alder pollen season, at the beginning and the peak of the birch pollen season and outside the pollen season. Mononuclear cells (MNC) were stimulated with allergen and polyclonal activators. For flow cytometric analysis, MNC were stained with monoclonal antibodies (MoAbs) against the cell surface markers CD3, CD8, CD14 and the intracellular cytokines IL-4, IL-5, IL-10, IL-12, IL-13, granulocyte macrophage-colony stimulating factor (GM-CSF) and IFN-gamma. RESULTS : In allergic patients, significant increases in clinical symptoms, use of medication, eosinophil numbers and birch-specific IgE were found during the pollen season. In vitro allergen stimulation increased the number of GM-CSF+ monocytes (P<0.01) and this increase was dependent on allergen exposure. The IL-4/IFN-gamma ratio rose (P<0.001) at the peak of birch pollen season and the ratio correlated with symptom scores during the birch season. In the CD4+ cell population, the numbers of GM-CSF+ cells were higher throughout the alder and birch seasons compared with outside the pollen season (P<0.05). No such changes were seen in the healthy controls. CONCLUSIONS : The main finding of our study was the increased percentage of GM-CSF+ monocytes in atopic subjects compared with healthy controls. In allergic patients, natural seasonal pollen exposure resulted in increased numbers of GM-CSF+ cells among both monocytes and CD4+ T cells. We have also shown that a seasonal change in Th2/Th1 cytokine ratio requires an adequate and prolonged allergen stimulation that is seen late in the pollen season.

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