| 1. |
- BALZARINI, J, et al.
(författare)
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Suppression of the breakthrough of human immunodeficiency virus type 1 (HIV-1) in cell culture by thiocarboxanilide derivatives when used individually or in combination with other HIV-1-specific inhibitors (i.e., TSAO derivatives)
- 1995
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424. ; 92:12, s. 5470-5474
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Tidskriftsartikel (refereegranskat)abstract
- Five structurally related thiophene and furane analogues of the oxathiin carboxanilide derivative NSC 615985 (UC84) (designated UC10, UC68, UC81, UC42, and UC16) were identified as potent inhibitors of HIV-1 replication in cell culture and HIV-1 reverse transcriptase activity. These compounds were markedly active against a series of mutant HIV-1 strains, containing the Leu-100-->Ile, Val-106-->Ala, Glu-138-->Lys, or Tyr-181-->Cys mutations in their reverse transcriptase. However, the thiocarboxanilide derivatives selected for mutations at amino acid positions 100 (Leu-->Ile), 101 (Lys-->Ile/Glu), 103 (Lys-->Thr/Asp) and 141 (Gly-->Glu) in the HIV-1 reverse transcriptase. The compounds completely suppressed HIV-1 replication and prevented the emergence of resistant virus strains when used at 1.3-6.6 microM--that is, 10- to 25-fold lower than the concentration required for nevirapine and bis(heteroaryl)piperazine (BHAP) U90152 to do so. If UC42 was combined with the [2',5'-bis-O-(tert-butyldimethylsilyl)-3'-spiro-5"-(4"-amino-1",2"- oxathiole-2",2"-dioxide)]-beta-D-pentofuranosyl (TSAO) derivative of N3-methylthymine (TSAO-m3T), virus breakthrough could be prevented for a much longer time, and at much lower concentrations, than if the compounds were used individually. Virus breakthrough could be suppressed for even longer, and at lower drug concentrations, if BHAP was added to the combination of UC42 with TSAO-m3T, which points to the feasibility of two- or three-drug combinations in preventing virus breakthrough and resistance development.
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| 2. |
- Grossi, Giorgio, et al.
(författare)
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The morning salivary cortisol response in burnout.
- 2005
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Ingår i: Journal of Psychosomatic Research. - : Elsevier. - 0022-3999 .- 1879-1360. ; 59:2, s. 103-111
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Tidskriftsartikel (refereegranskat)abstract
- The results of the present study indicate a dysregulation in hypothalamic-pituitary-adrenocortical axis (HPA axis) activity, characterised by elevated morning salivary cortisol levels, among female burnout patients. Among males, increased cortisol levels were observed among participants with moderate levels of burnout, but not among patients or healthy controls.
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| 3. |
- Verikas, Antanas, et al.
(författare)
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An intelligent system for tuning magnetic field of a cathode ray tube deflection yoke
- 2003
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Ingår i: Knowledge-Based Systems. - Amsterdam : Elsevier Science. - 0950-7051 .- 1872-7409. ; 16:3, s. 161-164
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Tidskriftsartikel (refereegranskat)abstract
- This short communication concerns identification of the number of magnetic correction shunts and their positions for deflection yoke tuning to correct the misconvergence of colours of a cathode ray tube. The misconvergence of colours is characterised by the distances measured between the traces of red and blue beams. The method proposed consists of two phases, namely, learning and optimisation. In the learning phase, the radial basis function neural network is trained to learn a mapping: correction shunt position→changes in misconvergence. In the optimisation phase, the trained neural network is used to predict changes in misconvergence depending on a correction shunt position. An optimisation procedure based on the predictions returned by the neural net is then executed in order to find the minimal number of correction shunts needed and their positions. During the experimental investigations, 98% of the deflection yokes analysed have been tuned successfully using the technique proposed.
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| 4. |
- Zamani, Leila
(författare)
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Extracellular metabolite fingerprinting of spent mammalian cell culture medium in relation to the quality of an expressed recombinant protein
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- This study demonstrates methodology for predicting the quality (in terms, for instance, of specific activities) of recombinant proteins expressed by mammalian cell cultures, at upstream process stages, using metabolic fingerprint analysis. Metabolites extracted from samples taken from a culture of a transfected Chinese Hamster Ovary (CHO) cell line expressing a recombinant protein in a bioreactor at various days of the cell culture process were analyzed by ultra high-pressure liquid chromatography-electrospray ionization-time of flight mass spectrometry (UHPLC-ESI-TOFMS). The LC-MS data were processed and the extracted information was correlated with the concentration of the active protein by partial least squares (PLS) regression, which revealed strong correlations between the LC-MS results and the concentration of active protein (R 2 = 0.99). The correlations between the LC-MS data and other parameters (glucose concentration, lactate concentration and the number of viable cells) were also studied. To obtain an overview of the data, Principal Component Analysis (PCA) was applied to the LC-MS data obtained from the samples to observe clustering or separation in the sample set. The PCA indicated that the LC-MS data obtained from samples from different days were significantly separated in temporal order from day 7 to day 28, according to the first Principal Component.
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| 5. |
- Huvila, Isto, Professor, 1976-
(författare)
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How Knowing Changes
- 2019
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Ingår i: Research Outside The Academy. - Basingstoke : Palgrave Macmillan. ; , s. 155-170
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- As the contemporary society is changing, knowing and its premises and conditions change together with it. This chapter explores this change and its implications to knowledge making. The main argument is that much of the contemporary experiences of change in information and knowledge practices can be understood from the perspective of how the conditions of (un)naming and (dis)trusting individuals, groups and institutions are changing. Further, it is suggested that the most significant issue may not necessarily be the change of knowing itself but rather the question of what is considered to count as knowing.
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| 6. |
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| 7. |
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| 8. |
- Emanuelsson, O., von Heijne, G., Elofsson, A., Cristóbal, S.
(författare)
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In silico prediction of the peroxisomal proteome in fungi, plants and animals
- 2003
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Ingår i: J. Mol. Biol.. ; 330, s. 443-456
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Tidskriftsartikel (refereegranskat)abstract
- In an attempt to improve our abilities to predict peroxisomal proteins, we have combined machine-learning techniques for analyzing peroxisomal targeting signals (PTS1) with domain-based cross-species comparisons between eight eukaryotic genomes. Our resul
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| 9. |
- Aleksandrov, E, et al.
(författare)
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Emerging streams of intitutional theory in accounting research : current status and future directions
- 2018
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- More than 30 years have passed since first calls to see accounting as social and institutional practice (Burchell et al., 1980; Hopwood, 1987; Hopwood & Miller, 1994). During these years, a significant number of theories have been striving to extend and challenge the hegemony of particular ways of seeing accounting (Jacobs, 2012) with acknowledging the need for inter-disciplinary approach and theoretical pluralism in contemporary studies. Within this theoretical pluralism, the institutional theory was recognized as one of the dominating theories in accounting research during last decades (Jacobs, 2012; Lounsbury, 2008; Modell, 2015).However, despite acknowledging the paradigmatic developments of institutional theory into several streams in organisational science (DiMaggio, 1988; Lawrence et al., 2009, 2011; Oliver, 1991; Thornton et al., 2012), few attempts were done to make a separate review of these streams and their value for accounting literature (Modell, 2009). Here, influential work of Lounsbury (2008) rather reflected organizational science literature with seeing accounting as a context which can give crucial insights into institutional and practice theories rather than vice versa. Jacobs (2012) in his study of theoretical pluralism in accounting research during 1992-2008 acknowledged the institutional theory domination but made few reflections on its progressions and implications for seeing accounting as social and institutional practice. And finally, more recent studies of Modell and his colleagues (Modell, 2015; Modell et al., 2017) made overviews of two streams of institutional theory (e.g. logic and work) and institutional theory combination possibility with other theories. While giving the valuable insights into understanding of issues related to theories combinations in accounting, these studies hardly review the accounting literature related to institutional theory streams which were criticised for apolitical nature but at the same time acknowledged as self-sufficient.Therefore, it seems that current studies rather ignore or criticize specific institutional theory streams, jumping into possible theory combinations agenda without revealing the value of these streams for accounting research. Such tendency may potentially lead to marginalization of accounting knowledge (Jacobs, 2016; Jacobs & Cuganesan, 2014) with the use of these streams under the quest for contribution to the interdisciplinary literature.In this regard, this paper aims to reflect upon the emerging streams of institutional theory, their application and influence on the accounting research of the last decade. Specifically, the paper’s ambition is to review and understand the role of institutional logics (IL), institutional entrepreneurship (IE) and institutional work (IW) in accounting research. These streams of institutional theory become increasingly valuable in accounting research for their explanatory capacities. But at the same time, but their application, especially in a combined form, can also raise criticalities within theoretical pluralism agenda. Therefore, we construct our research questions as:How have the emerging stream of institutional theory (IL, IE and IW) been used by accounting scholars?How have these streams influenced accounting research? What could be done in the future accounting research using emerging streams of institutional theory?In order to answer these research questions, we compile a structured literature review (Massaro et al., 2016) of articles published in scientific journals in the accounting field (levels 4 and 3 in ABS list). As a frame for analysis of the paper, we use conceptual clarification and analytical framework proposed by Lukka and Vinnari (2014) who made a distinction between domain theory and method theory in order to capture the relations between common knowledge about accounting as a social practice and the theories used to extend this knowledge. Applying to our paper, domain theory refers particularly to a set of knowledge about accounting as social and institutional practice. Method theory (i.e. IL, IE, IW) is a meta-level conceptual system, which can offer alternative perspectives and add new understanding/insights into domain theory. Following this analytical distinction, we examine the way how current accounting literature utilizes the selected institutional streams in domain-method dimension, how the theory or their combination (if any) are justified and used in a flow of argument for understanding of accounting practice.
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| 10. |
- Baumann, Ulrich, et al.
(författare)
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Lectin-reactive alpha-fetoprotein in patients with tyrosinemia type I and hepatocellular carcinoma.
- 2006
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Ingår i: Journal of pediatric gastroenterology and nutrition. - : Ovid Technologies (Wolters Kluwer Health). - 0277-2116. ; 43:1, s. 77-82
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Tidskriftsartikel (refereegranskat)abstract
- Despite the introduction of 2-(2-nitro-4-trifluormethyl-benzoyl)-1,3-cyclohexandion into the treatment of hereditary tyrosinemia type I (HT1), patients remain at risk of developing hepatocellular carcinoma (HCC). Serial total alpha-fetoprotein (AFP) levels are used to monitor the individual patient. Lectin-reactive alpha-fetoprotein (L3-AFP) is an AFP isoform that is expressed by malignant liver tumors. We investigated whether the analysis of L3-AFP could lead to earlier detection of HCC in HT1 compared with judgement based on total AFP alone. AFP electrophoresis using lectin-containing agarose gel identifies L3-AFP by the affinity of its specific carbohydrate chain to lectin. We report the retrospective analysis of sequential serum samples of 12 patients with HT1 and histologically proven HCC. AFP isoforms could be identified in all 12 patients. In 6 patients, the L3-AFP increased before the total AFP. In 3 patients, the rise in L3-AFP was parallel to the rise of total AFP; and in 3 patients, the L3-AFP was raised after the total AFP or did not increase at all. We were able to identify 6 of 12 patients with an early increase in the new tumor marker. Lectin-affinity electrophoresis may have a role in discriminating benign liver disease from HCC in HT1. We suggest the further evaluation of L3-AFP in HT1.
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