| 211. |
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| 212. |
- Nordström, Peter, et al.
(författare)
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Type of physical activity, muscle strength, and pubertal stage as determinants of bone mineral density and bone area in adolescent boys.
- 1998
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Ingår i: Journal of Bone and Mineral Research. - : Wiley. - 0884-0431 .- 1523-4681. ; 13:7, s. 1141-8
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Tidskriftsartikel (refereegranskat)abstract
- The present study was conducted to evaluate the influence of different types of weight-bearing physical activity, muscle strength, and puberty on bone mineral density (BMD, g/cm2) and bone area in adolescent boys. Three different groups were investigated. The first group consisted of 12 adolescent badminton players (age 17.0 +/- 0.8 years) training for 5.2 +/- 1.9 h/week. The second group consisted of 28 ice hockey players (age 16.9 +/- 0.3 years) training for 8.5 +/- 2.2 h/week. The third group consisted of 24 controls (age 16.8 +/- 0.3 years) training for 1.4 +/- 1.4h/week. The groups were matched for age, height, and pubertal stage. BMD, bone mineral content (BMC, g), and the bone area of the total body, lumbar spine, hip, femur and tibia diaphyses, distal femur, proximal tibia, and humerus were measured using dual-energy X-absorptiometry. When adjusting for the difference in body weight between the groups, the badminton players were found to have significantly higher BMD (p < 0.05) of the trochanter and distal femur compared with the ice hockey players despite a significantly lower weekly average training. The badminton players had higher BMD compared with the control with the control group at all weight-bearing BMD sites, except at the diaphyses of the femur and tibia and lumbar spine. The independent predictors of bone density were estimated by adjusting BMC for the bone area in a multivariate analysis among all subjects (n = 64). Accordingly, the bone density of all sites except the spine was significantly related to muscle strength and height, and the bone density of the total body, neck, trochanter, distal femur, and proximal tibia was significantly related to type of physical activity (beta = 0.09-0.33, p < 0.05). The bone area values at different sites were strongly related to muscle strength and height and less strongly related to the type of physical activity and pubertal stage. In conclusion, it seems that during late puberty in adolescent boys the type of weight-bearing physical activity is an important determinant of bone density, while the bone area is largely determined by parameters related to body size. The higher BMD at weight-bearing sites in badminton players compared with ice hockey players, despite significantly less average weekly training, indicates that physical activity including jumps in unusual directions has a great osteogenic potential.
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| 213. |
- Norrback, Karl-Fredrik, et al.
(författare)
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Telomerase activity in Hodgkin's disease
- 1998
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Ingår i: Blood. - 0006-4971 .- 1528-0020. ; 92:2, s. 567-573
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Tidskriftsartikel (refereegranskat)abstract
- Telomere maintenance executed by the action of telomerase seems to be a prerequisite for immortalization. Telomerase is found in most cell lines and malignant tumors. A telomerase-independent mechanism for telomere maintenance in Hodgkin's disease has been proposed in the absence of detectable telomerase activity. In this study, telomerase activity was detected in 31 of 77 Hodgkin's disease samples and a strong correlation between eosinophilia and absence of detectable telomerase activity was found. Purified eosinophils and specifically eosinophil-derived neurotoxin and eosinophilic cationic protein, both ribonucleases, were found to degrade telomerase. Purified neutrophils also exhibited weak telomerase degradative activity. Reanalysis of previously telomerase-negative Hodgkin's disease samples with eosinophilia using ribonuclease inhibitors resulted in the detection of telomerase activity. Ribonuclease-containing cells in vivo thus have a considerable impact on the detectability of telomerase. In Hodgkin's disease samples without eosinophilia, 24 of 27 exhibited telomerase activity at decreased levels compared with non-Hodgkin's lymphomas and at increased levels compared with reactive nodes indicative of a telomerase positive tumor component in Hodgkin's disease. Telomerase positivity of the Hodgkin's and Reed-Sternberg cells in vivo was also supported by high levels of telomerase expression in Hodgkin's disease cell lines. Based on our data, Hodgkin's lymphomas are potential targets for antitelomerase therapy.
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| 214. |
- Novak, R, et al.
(författare)
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Penicillin tolerance genes of Streptococcus pneumoniae : the ABC-type manganese permease complex Psa.
- 1998
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Ingår i: Molecular Microbiology. - 0950-382X .- 1365-2958. ; 29:5, s. 1285-1296
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Tidskriftsartikel (refereegranskat)abstract
- Downregulation of the major autolysin in Streptococcus pneumoniae leads to penicillin tolerance, a feature that is characterized by the ability to survive but not grow in the presence of antibiotic. Screening a library of mutants in pneumococcal surface proteins for the ability to survive 10x minimum inhibitory concentration (MIC) of penicillin revealed over 10 candidate tolerance genes. One such mutant contained an insertion in the known gene psaA, which is part of the psa locus. This locus encodes an ABC-type Mn permease complex. Sequence analysis of adjacent DNA extended the known genetic organization of the locus to include two new open reading frames (ORFs), psaB, which encodes an ATP-binding protein, and psaC, which encodes a hydrophobic transmembrane protein. Mutagenesis of psaB, psaC, psaA and downstream psaD resulted in penicillin tolerance. Defective adhesion and reduced transformation efficiency, as reported previously for a psaA- mutant, were phenotypes shared by psaB-, psaC- and psaD- knockout mutants. Western blot analysis demonstrated that the set of mutants expressed RecA, but none of them showed translation of the autolysin gene, which is located downstream of recA. The addition of manganese (Mn) failed to correct the abnormal physiology. These results suggest that this ABC-type Mn permease complex has a pleiotropic effect on pneumococcal physiology including adherence and autolysis. These are the first genes suggested as being involved in triggering autolysin. The results raise the possibility that loss of function of PsaA, by vaccine-induced antibody for instance, may promote penicillin tolerance.
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| 215. |
- Nygård, Louise, et al.
(författare)
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The Relationship Between Cognition and Daily Activities in Cases of Mild Alzheimer's Disease
- 1998
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Ingår i: Scandinavian Journal of Occupational Therapy. - : Informa UK Limited. - 1103-8128 .- 1651-2014. ; 5:4, s. 160-166
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Tidskriftsartikel (refereegranskat)abstract
- The purpose of this study was to investigate the relationship between cognitive functions, assessed by neuropsychological tests, and instrumental activities of daily living (IADL) ability, assessed by the Assessment of Motor and Process Skills (AMPS) in persons with mild Alzheimer's disease. Fifty-two participants were included in the study. We found significant relationships between the AMPS IADL process ability and Factors 1 (perceptual speed, visuoconstructive ability) and 4 (secondary memory). The AMPS IADL motor ability showed a significant relationship to Factors 1 and 3 (logical memory). The cognitive factors explained 24 26% of the variability in AMPS IADL ability. The results indicate that cognitive functions had a significant impact on IADL ability in this population. However, as 74–76% of the variability in the IADL ability was left unaccounted for by the cognitive functions, it is important to assess both cognitive functions and IADL ability when investigating persons with mild dementia. Further study of the complex interaction between a variety of influencing factors is needed to better understand the consequences of dementia on everyday life functioning.
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| 216. |
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| 217. |
- Olofsson, Britta, et al.
(författare)
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Nurses' narratives about using coercion in psychiatric care
- 1998
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Ingår i: Journal of Advanced Nursing. - : Wiley. - 0309-2402 .- 1365-2648. ; 28:1, s. 45-53
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Tidskriftsartikel (refereegranskat)abstract
- To illuminate nurses' experience of using coercion against psychiatric patients, a phenomenological hermeneutic study was conducted with seven registered nurses and seven enrolled mental nurses. The findings revealed that these nurses desired to be seen by themselves, their co-workers and patients as doing good, being good and providing good care but they experienced the use of coercion as not good. This conflict made it difficult for these nurses to question the use of coercion and consider alternative solutions. The theory of change by Watzlawick et al. (1974) served as a theoretical framework for the interpretation of all interviews regarded as one text. In order to explore alternatives to the use of coercion in psychiatric care, nurses need assistance to step outside the traditional way of regarding coercion in psychiatric care and seek to create new solutions.
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| 218. |
- Olofsson, Katarina, et al.
(författare)
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The surface epithelium of recurrent infected palatine tonsils is rich in gammadelta T cells
- 1998
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Ingår i: Clinical and Experimental Immunology. - : Oxford University Press (OUP). - 0009-9104 .- 1365-2249. ; 111:1, s. 36-47
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Tidskriftsartikel (refereegranskat)abstract
- Using a large panel of MoAbs in quantitative morphometric analysis of immunohistochemically stained tissue sections, we compared the frequency and distribution of immune cells in palatine tonsils from patients with recurrent tonsillitis (RT) and patients with idiopathic tonsillar hypertrophy (ITH). We found that differences between the two patient groups in leucocyte populations were limited to the surface epithelium, whereas the cellular composition of interfollicular and follicular areas was similar. Most intraepithelial lymphocytes were CD8+ T cells in both groups. However, the number of intraepithelial T cells was significantly higher in RT compared with ITH. This was due to a selective increase in the number of intraepithelial CD8+ gammadelta T cells utilizing Vdelta1 and Vgamma9. In both patient groups the majority of the intraepithelial gammadelta T cells expressed Vdelta1 and Vgamma9. Subepithelially, gammadelta T cells utilizing Vgamma9 dominated over cells utilizing Vgamma8, while equal proportions expressed Vdelta1 and Vdelta2. These results suggest that cells utilizing the otherwise rare combination Vdelta1/Vgamma9 in their T cell receptors (TCR) may constitute a major gammadelta T cell population in palatine tonsils and are probably reactive to antigens specific to the tonsillar milieu. Furthermore, they indicate that preferentially this gammadelta T cell subpopulation is involved in immune reactions within the surface epithelium in RT. We speculate that gammadelta T cells are involved in clearing infectious bacteria at the tonsillar surface and in limiting inflammatory responses in the tonsils. Both local expansion and infiltration of blood cells probably contribute to the high numbers of gammadelta T cells in RT patients.
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| 219. |
- Olofsson, Thomas, 1968-, et al.
(författare)
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A method for predicting the annual building heating demand based on limited performance data
- 1998
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Ingår i: Energy and Buildings. - : Elsevier. - 0378-7788 .- 1872-6178. ; 28:1, s. 101-108
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Tidskriftsartikel (refereegranskat)abstract
- In this paper, we present an investigation of the possibility to use a neural network combined with a quasi-physical description in order to predict the annual supplied space heating demand (P) for a number of small single family buildings located in the North of Sweden. As a quasi-physical description for P, we used measured diurnal performance data from a similar building or simulated data from a steady state energy simulation software. We show that the required supplied space heating demand may be predicted with an average accuracy of 5%. The predictions were based on access to measured diurnal data of indoor and outdoor temperatures and the supplied heating demand from a limited time period, ranging from 10 to 35 days. The prediction accuracy was found to be almost independent of what time of the year the measurements were obtained from, except for periods when the supplied heating demand was very small. For models based on measurements from May and fo some buildings from April and September, the prediction was less accurate.
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| 220. |
- Olofsson, Thomas, 1968-, et al.
(författare)
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Energy load predictions for buildings based on a total demand perspective
- 1998
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Ingår i: Energy and Buildings. - : Elsevier. - 0378-7788 .- 1872-6178. ; 28:1, s. 109-116
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Tidskriftsartikel (refereegranskat)abstract
- The outline of this work was to develop models for single family buildings, based on a total energy demand perspective, i.e., building-climate-inhabitants. The building-climate part was included by using a commercial dynamic energy simulation software. Whereas the influence from the inhabitants was implemented in terms of a predicted load for domestic equipment and hot water preparation, based on a reference building. The estimations were processed with neural network techniques. All models were based on access to measured diurnal data from a limited time period, ranging from 10 to 35 days. The annual energy predictions were found to be improved, compared to models based on only a building-climate perspective, when the domestic load was included. For periods with a small heating demand, i.e., May-September, the average accuracy was 7% and 4% for the heating and total energy load, respectively, whereas for the rest of the year the accuracy was on average 3% for both heating and total energy load.
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