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Sökning: db:Swepub > Mattiasson Bo > (2000-2004) > Engelska

  • Resultat 61-70 av 103
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61.
  • Liu, Jing, et al. (författare)
  • On-line monitoring of a two-stage anaerobic digestion process using a BOD analyzer
  • 2004
  • Ingår i: Journal of Biotechnology. - : Elsevier BV. - 1873-4863 .- 0168-1656. ; 109:3, s. 263-275
  • Tidskriftsartikel (refereegranskat)abstract
    • A computer-controlled biochemical oxygen demand (BOD) analyzer has been developed for fast estimation of biochemical oxygen demand (BOD,,) automatically with the purpose of on-line monitoring of a process for conversion of biomass under field conditions. The instrument was tested by on-line monitoring of the connecting stream between two stages of a two-stage anaerobic process in laboratory scale. In the first stage, hydrolysis of sugar beet leaves and its conversion into volatile fatty acids and other low molecular weight substrates took place. The effluent from the first reactor was used as a feed stream to the second stage, i.e. an anaerobic contact reactor. The feed stream was sampled intermittently, diluted and analyzed by the BOD analyzer automatically in order to estimate the organic loading rate to the reactor. The results from this study demonstrated that the BOD analyzer could be a stand-alone and promising sensor device for rapid on-line monitoring of easily biodegradable organic substances in biological treatment processes. (C) 2004 Elsevier B.V. All rights reserved.
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62.
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63.
  • Liu, Jing, et al. (författare)
  • Short-term BOD (BODst) as a parameter for on-line monitoring of biological treatment process Part 1. A novel design of BOD biosensor for easy renewal of bio-receptor
  • 2004
  • Ingår i: Biosensors & Bioelectronics. - : Elsevier BV. - 1873-4235 .- 0956-5663. ; 20:3, s. 562-570
  • Tidskriftsartikel (refereegranskat)abstract
    • A novel design of a biochemical oxygen demand (BOD) biosensor has been developed for on-line monitoring of easily biodegradable organic compounds in aqueous samples. The biological recognition element of the sensor could be easily renewed by injecting new bacterial paste without disassembling the sensor system. The sensor measurements were carried out in the initial-rate mode using a flow injection (FI) system, resulting in 60s for one sample analysis followed by a recovery time less than 10 min. The sensor performance achieved showed a wide detection linearity over the range of 5-700 mg BOD5.1(-1) and a generally good agreement between the BOD values estimated by the biosensor and the conventional 5-day test. Furthermore, the precision test was in the control range (i.e. repeatability less than or equal to+/-7.5%, reproducibility less than or equal to+/-7.3%). The sensor could be used over 1 week in continuous test, however, the best performance was found within the first 24 h where standard deviation of the sensor response was +/-2.4%. The design of the sensor allows easy and fast renewal of the cells used as sensing elements. Replacement of biological recognition element and calibration of the sensor responses can be performed in a rather simple procedure on a daily regular basis. By using a mixed culture as the bio-receptor, one gets a sensor that reacts to a wide range of substrates. The new sensor construction will thus allow fast and convenient replacement of the bio-receptor and on-line assay of a broad range of substrates. This makes the sensor being an interesting and promising candidate for on-line monitoring of biological treatment process. (C) 2004 Elsevier B.V. All rights reserved.
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64.
  • Liu, Jing, et al. (författare)
  • Short-term BOD (BODst) as a parameter for on-line monitoring of biological treatment process. Part II: Instrumentation of integrated flow injection analysis (FIA) system for BODst estimation
  • 2004
  • Ingår i: Biosensors & Bioelectronics. - : Elsevier BV. - 1873-4235 .- 0956-5663. ; 20:3, s. 571-578
  • Tidskriftsartikel (refereegranskat)abstract
    • An instrument with integrated flow injection analysis (FIA) system has been developed for on-line monitoring a process for conversion of biomass under field condition. The instrument consists of a newly designed biosensor for easy renewal of the bio-receptor without disassembling the sensor, a FIA controller for controlling the analysis operations, and a computer-based data acquisition system for data recording and processing. The instrument performed a sequence operations automatically including preparation of sample in the desired concentration, sample loading, sample injection, signal recording, data processing, and self-cleaning of the system. This makes the instrument being an interesting and promising device for on-line process monitonng. (C) 2004 Elsevier B.V. All rights reserved.
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65.
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66.
  • Lozinsky, Vladimir I, et al. (författare)
  • Polymeric Cryogels as Promising Materials of Biotechnological Interest.
  • 2003
  • Ingår i: Trends in Biotechnology. - : Elsevier BV. - 0167-7799. ; 21:10, s. 445-451
  • Forskningsöversikt (refereegranskat)abstract
    • Cryogels are gel matrices that are formed in moderately frozen solutions of monomeric or polymeric precursors. Cryogels typically have interconnected macropores (or supermacropores), allowing unhindered diffusion of solutes of practically any size, as well as mass transport of nano- and even microparticles. The unique structure of cryogels, in combination with their osmotic, chemical and mechanical stability, makes them attractive matrices for chromatography of biological nanoparticles (plasmids, viruses, cell organelles) and even whole cells. Polymeric cryogels are efficient carriers for the immobilization of biomolecules and cells.
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67.
  • Lozinsky, Vladimir I, et al. (författare)
  • Synthesis and studies of N-vinylcaprolactam/N-vinylimidazole copolymers taht exhibit the ”proteinlike” behaviour in aquesous media
  • 2003
  • Ingår i: Macromolecules. - : American Chemical Society (ACS). - 0024-9297 .- 1520-5835. ; 36:19, s. 7308-7323
  • Tidskriftsartikel (refereegranskat)abstract
    • Proteinlike copolymers were first predicted by the computer-aided biomimetic design (Physica A 1998, 249, 253-261). These copolymers consist of comonomer units of differing hydrophilicity/hydrophobicity. Heterogeneous blockiness, characteristic for such copolymers, facilitates chain folding with the formation of specific spatial packing: a dense core consisting of hydrophobic units and a polar shell formed by hydrophilic units. This paper describes the synthesis of N-vinylcaprolactam/N-vinylimidazole copolymers via the redox-initiated radical copolymerization in the medium of 10% aqueous DMSO at the temperatures both below and above the phase separation threshold. The synthesized macromolecular products were separated into thermally precipitating and nonprecipitating fractions. Their molecular weight characteristics were evaluated using size-exclusion chromatography; their comonomer composition was determined from 1H NMR spectra of copolymers dissolved in DMSO-d6. The temperature-dependent behavior of copolymer macromolecules in water was investigated by thermonephelometry, high-sensitivity differential scanning calorimetry, and 1H NMR spectroscopy of the copolymers dissolved in D2O. It was shown that thermally nonprecipitating copolymer fractions obtained at initial comonomer molar ratios of 85:15 and 90:10 can be identified as proteinlike copolymers.
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68.
  • Lozinsky, Vladimir, et al. (författare)
  • The potential of polymeric cryogels in bioseparation
  • 2001
  • Ingår i: Bioseparation. - 1573-8272. ; 10:4-5, s. 163-188
  • Tidskriftsartikel (refereegranskat)abstract
    • This is a review discussing the production and properties of cryogels (from the Greek κριoσ (kryos) meaning frost or ice), immobilization of ligands in cryogels and the application of affinity cryogels in bioseparation. Cryotropic gel formation proceeds in a non-frozen liquid microphase existing in the macroscopically frozen sample. Due to the cryoconcentration of gel precursors in the non-frozen liquid microphase, cryogelation is characterised by a decrease in the critical concentration of gelation and an increase in gelation rates compared with traditional gelation at temperatures above freezing point. Cryogels can be obtained through the formation of both physically and covalently cross-linked heterogeneous polymer networks. Interconnected systems of macropores and sponge-like morphology are typical for cryogels, allowing unhindered diffusion of solutes of practically any size. Most of the water present in spongy cryogels is capillary bound and can be removed mechanically by squeezing. The properties of cryogels can be regulated by the temperature of cryogelation, the time the sample is kept in a frozen state and freezing/thawing rates, by the nature of the solvent and by the use of soluble and insoluble additives. The unique macroporous morphology of cryogels, in combination with osmotic, chemical and mechanical stability, makes them attractive matrices for chromatography of large entities such as protein aggregates, membrane fragments, viruses, cell organells and even whole cells. Special attention is given to immunosorption of viruses on cryogel-based sorbents. As chromatographic materials, cryogels can be used both in bead form and as spongy cylindrical blocks (monoliths) synthesized inside the chromatographic column. The macroporous nature of cryogels is also advantageous for their application as matrices in the immobilization of biocatalysts operating in both aqueous and organic solvents. New potential applications of cryogels are discussed.
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69.
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70.
  • Mattiasson, Bo, et al. (författare)
  • Process monitoring during protein purification.
  • 2003
  • Ingår i: Isolation and purifiaction of proteins (Biotechnology and Bioprocessing ; 27). - 0824707265
  • Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
    • Abstract is not available
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  • Resultat 61-70 av 103

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