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  • Result 21-30 of 547
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21.
  • Puschmann, Till B., et al. (author)
  • HB-EGF affects astrocyte morphology, proliferation, differentiation, and the expression of intermediate filament proteins
  • 2014
  • In: Journal of Neurochemistry. - : Wiley. - 1471-4159 .- 0022-3042. ; 128:6, s. 878-889
  • Journal article (peer-reviewed)abstract
    • Heparin-binding epidermal growth factor-like growth factor (HB-EGF), a vascular-derived trophic factor, belongs to the epidermal growth factor (EGF) family of neuroprotective, hypoxia-inducible proteins released by astrocytes in CNS injuries. It was suggested that HB–EGF can replace fetal calf serum (FCS) in astrocyte cultures. We previously demonstrated that in contrast to standard 2D cell culture systems, Bioactive3D culture system, when used with FCS, minimizes the baseline activation of astrocytes and preserves their complex morphology. Here, we show that HB-EGF induced EGF receptor (EGFR) activation by Y1068 phosphorylation, Mapk/Erk pathway activation, and led to an increase in cell proliferation, more prominent in Bioactive3D than in 2D cultures. HB-EGF changed morphology of 2D and Bioactive3D cultured astrocytes toward a radial glia-like phenotype and induced the expression of intermediate filament and progenitor cell marker protein nestin. Glial fibrillary acidic protein (GFAP) and vimentin protein expression was unaffected. RT-qPCR analysis demonstrated that HB-EGF affected the expression of Notch signaling pathway genes, implying a role for the Notch signaling in HB-EGF-mediated astrocyte response. HB-EGF can be used as a FCS replacement for astrocyte expansion and in vitro experimentation both in 2D and Bioactive3D culture systems; however, caution should be exercised since it appears to induce partial de-differentiation of astrocytes.HB-EGF (heparin-binding EGF-like growth factor) was previously suggested to replace serum, a common and undefined component in primary astrocyte cultures. We show that both in standard 2D and in our newly developed Bioactive3D culture system, HB-EGF affects astrocyte morphology, proliferation, gene/protein expression and leads to partial de-differentiation of astrocytes. Thus, HB-EGF should only be used with caution as a serum replacement in astrocyte cultures.
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23.
  • Andersson, Cristina, 1969, et al. (author)
  • Effect of different temperature cycle profiles on the crack propagation and microstructural evolution of Sn-3.8Ag-0.7Cu solder joints
  • 2005
  • In: 15th European Microelectronics and Packaging Conference and Exhibition, EMPC 2005 - Conference Programme and Proceedings. ; 2005, s. 523-528
  • Conference paper (peer-reviewed)abstract
    • Temperature cycling of real electronic components was carried out in a systematic manner at two different temperature profiles and in a single-chamber Heraeus climate cabinet. The first temperature profile ranged between -55°C and 100°C and the second between 0°C and 100°C. Top-side SMD components (chip resistors and Ball Grid Arrays (BGA)) were soldered with Sn-3.8Ag-0.7Cu lead free solder paste. Crack initiation and propagation was analyzed after every 500 cycles for each temperature cycling profile. Totally, 6500 cycles were run at both temperature profiles. Finite element modeling (FEM) calculations, for the analysis of strain and stress of 1206 chip joints were used to corroborate the experimental work results, especially regarding the crack initiation sites. Cracks were firstly visible for the temperature cycling ranging between -55°C and 100°C. The cracks observed were also visibly smaller for the temperature profile ranging between 0°C and 100°C, concluding that crack initiation and propagation was slightly slower for this temperature profile. Cracks continued to propagate as a function of temperature cycles. The reason for this difference in crack initiation and propagation was also analyzed.
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29.
  • Fu, Ying, 1964, et al. (author)
  • Conductive adhesives
  • 2004
  • In: Lead free electronics. ; , s. pp 285-351
  • Book chapter (other academic/artistic)
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  • Result 21-30 of 547
Type of publication
conference paper (329)
journal article (195)
book chapter (11)
research review (8)
patent (2)
reports (1)
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book (1)
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Type of content
peer-reviewed (515)
other academic/artistic (32)
Author/Editor
Fu, Yifeng, 1984 (95)
Ye, L (70)
Wang, Teng, 1983 (46)
Andersson, Cristina, ... (44)
Ye, Lilei (41)
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Cheng, Zhaonian, 194 ... (37)
Zhang, Yong, 1982 (37)
Lai, Zonghe, 1948 (34)
Lu, Xiuzhen (34)
Jeppson, Kjell, 1947 (32)
Zhang, Yan, 1976 (30)
Zhang, Yan (29)
Wang, Nan, 1988 (29)
Zandén, Carl, 1984 (29)
Carlberg, Björn, 198 ... (27)
Chen, Si, 1981 (26)
Sun, Shuangxi, 1986 (25)
Jiang, Di, 1983 (25)
Edwards, Michael, 19 ... (23)
Zhang, Y. (22)
Zehri, Abdelhafid, 1 ... (20)
Mu, Wei, 1985 (19)
Ye, Lilei, 1970 (18)
Nylander, Andreas, 1 ... (18)
Kabiri Samani, Majid ... (16)
Wang, Nan (16)
Luo, Xin, 1983 (16)
Hu, Zhili, 1983 (16)
Chen, S. (15)
Hansson, Josef, 1991 (15)
Huang, S. (14)
Sun, Peng, 1979 (13)
Larsson, Ragnar, 196 ... (12)
Wang, Xitao (12)
Shangguan, Dongkai (12)
Bao, Jie (12)
Huang, Shirong (12)
Nkansah, Amos (12)
Liu, Ya, 1991 (12)
Murugesan, Murali, 1 ... (12)
Nilsson, Torbjörn, 1 ... (11)
Yuan, G. (11)
Gao, Yulai (10)
Zhai, Qijie (10)
Andrae, Anders, 1973 (10)
Lu, X. (9)
Cao, Liqiang, 1974 (9)
Cui, H (9)
Lu, Hongbin (9)
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University
Chalmers University of Technology (547)
University of Gothenburg (11)
Uppsala University (6)
RISE (3)
Royal Institute of Technology (1)
Linköping University (1)
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Jönköping University (1)
Lund University (1)
Karlstad University (1)
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Language
English (547)
Research subject (UKÄ/SCB)
Engineering and Technology (491)
Natural sciences (115)
Medical and Health Sciences (13)
Agricultural Sciences (1)

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