| 1. |
- Banerjee, S, et al.
(författare)
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Amperometric Biosensor for estimation of Glucose-6-phosphate Using Prussian Blue Nanoparticles.
- 2013
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Ingår i: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 439:2, s. 194-200
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Tidskriftsartikel (refereegranskat)abstract
- Glucose-6-phosphateplays an important role in carbohydrate metabolism of all living organisms.Compared to the conventional analytical methods available for estimation of glucose-6-phosphate,the biosensors having relative simplicity, specificity, low-cost and fastresponse time are a promising alternative. We have reported a glucose-6-phosphatesensor based on screen-printed electrode utilizing Prussian blue nanoparticlesand enzymes, glucose-6-phosphate dehydrogenase and glutathione reductase. The Prussianblue nanoparticles acted as a mediator enhancing the rate of electrochemical responses.The Fourier transforminfrared spectroscopy and energy-dispersiveX-ray spectroscopy study confirmed the formation of Prussian blue, whereas, the atomic forced microscopy revealed that Prussian bluenanoparticles were about 25-30 nm in diameter. To obtainmaximum amperometric response, optimization studies were conducted for pH,enzyme and cofactor loading. The proposed glucose-6-phosphate biosensor showed goodstability, rapid response time and broad linear response in the range of 0.01-1.25mM and detection limit of 6.3 mM. The biosensor also worked well for serum samples and exhibitedexcellent anti-interference ability.
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| 2. |
- Bossi, Alesandra, et al.
(författare)
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Ice matrix inreconfigurable microfluidic systems
- 2013
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Ingår i: Laser physics. - : Institute of Physics (IOP). - 1054-660X .- 1555-6611. ; 23:7
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Tidskriftsartikel (refereegranskat)abstract
- Microfluidic devices find many applications in biotechnologies. Here, we introduce a flexible and biocompatible microfluidic ice-based platform with tunable parameters and configuration of microfluidic patterns that can be changed multiple times during experiments. Freezing and melting of cavities, channels and complex relief structures created and maintained in the bulk of ice by continuous scanning of an infrared laser beam are used as a valve action in microfluidic systems. We demonstrate that pre-concentration of samples and transport of ions and dyes through the open channels created can be achieved in ice microfluidic patterns by IR laser-assisted zone melting. The proposed approach can be useful for performing separation and sensing processes in flexible reconfigurable microfluidic devices.
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| 3. |
- Cao, S., et al.
(författare)
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Hierachically Structured Hollow Silica Spheres for High Efficiency Immobilization of Enzymes
- 2013
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Ingår i: Advanced Functional Materials. - : Wiley. - 1616-301X .- 1616-3028. ; 23:17, s. 2162-2167
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Tidskriftsartikel (refereegranskat)abstract
- In this work, the first example of a hierarchically structured hollow silica system is reported without any chemical modification to the enzyme involved in the process. The leaching of the physically adsorbed enzyme is substantially restrained in comparison to pure hollow silica supports. The hierarchical architecture is composed of the ordered hollow silica spheres with a shell-in-shell structure. This rationally integrated architecture, which serves as the host for glucose oxidase immobilization, displays many significant advantages, including increases in mechanical stability, enzyme loading, and bioactivity, and a decrease in enzyme leaching compared to existing pure hollow silica matrices. This facilitates further multifarious applications for enhanced enzyme immobilization, biosensors, and biocatalysis.
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| 4. |
- Karimian, N, et al.
(författare)
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On/off-switchable electrochemical sensor for folicacid based on molecularly imprinted technology.
- 2013
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Ingår i: Electrochemistry communications. - : Elsevier BV. - 1388-2481 .- 1873-1902. ; 36, s. 92-95
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Tidskriftsartikel (refereegranskat)abstract
- The combination of smart polymers with molecular imprinting offers a powerful tool to design more effective sensors and medical devices. In this study, a temperature sensitive amine-terminated poly(N-isopropylacrylamide) block with (N,N'-methylenebisacrylamide) cross-linker along with o-phenylenediamine was electropolymerised on a gold electrode in the presence of folic acid (FA) as template to produce an on/off-switchable molecularly imprinted polymer (MIP) affinity sensor for folic acid. Differential pulse voltammetry and cyclic voltammetry were used to characterise the FA-imprinted layer. Incubation of the MIP-modified electrode with FA resulted in a suppression of the ferro/ferricyanide redox process. The highest sensitivity of this temperature gated on/off-switchable folic acid sensor was achieved at 22 °C. Such switchable affinity materials offer considerable potential for the design of highly selective and controllable biosensors and immunoassays.
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| 5. |
- Li, He, et al.
(författare)
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Electrochemical immunosensor with N-doped graphene-modified electrode for label-free detection of the breast cancer biomarker CA 15-3
- 2013
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Ingår i: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 43, s. 25-29
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Tidskriftsartikel (refereegranskat)abstract
- Highly sensitive and label-free detection of the biomarker carbohydrate antigen 15-3 (CA 15-3) remains a challenge in the diagnosis of breast cancer. Here, a novel electrochemical immunosensor capable of sensitive and label-free detection of CA 15-3 is reported. This unique immunosensor, equipped with a highly conductive graphene (i.e., N-doped graphene sheets)-modified electrode, exhibited significantly increased electron transfer and high sensitivity toward CA 15-3. This novel immunosensor, with a low detection limit of 0.012U/mL, worked well over a broad linear range of 0.1-20U/mL. Unlike conventional immunosensors, which usually involve complicated label processing and time-consuming separations, the use of highly conductive graphene avoids the need for labels and is simple in nature. The strategy developed for this immunosensor provides a promising approach for clinical research and diagnostic applications.
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| 6. |
- Li, Songjun, et al.
(författare)
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A Catalytic and Positively Thermosensitive Molecularly Imprinted Polymer
- 2011
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Ingår i: Advanced Functional Materials. - : John Wiley and Sons, Ltd. - 1616-301X .- 1616-3028. ; 21:6, s. 1194-1200
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Tidskriftsartikel (refereegranskat)abstract
- A catalytic and positively thermosensitive molecularly imprinted polymer is reported. This unique imprinted polymer was composed of 4-nitrophenyl phosphate-imprinted networks that exhibited a thermosensitive interpolymer interaction between poly(2-trifluoromethylacrylic acid) (PTFMA) and poly(1-vinylimidazole) (PVI), which contains catalytically active sites. At a relatively low temperature (such as 20 degrees C), this imprinted polymer did not demonstrate significant catalytic activity for the hydrolysis of 4-nitrophenyl acetate due to the interpolymer complexation between PVI and PTFMA, which blocked access to the active sites of PVI and caused shrinking of the polymer. Conversely, at higher temperatures (such as 40 degrees C), this polymer showed significant catalytic activity resulting from the dissociation of the interpolymer complexes between PVI and PTFMA, which facilitated access to the active sites of PVI and inflated the polymer. Unlike previously reported poly(N-isopropylacrylamide)-based molecularly imprinted polymers, which demonstrated decreased molecular recognition and catalytic activity with increased temperatures, i.e., negatively thermosensitive molecular recognition and catalysis abilities, this imprinted polymer exploits the unique interpolymer interaction between PVI and PTFMA, enabling the reversed thermal responsiveness.
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| 7. |
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| 8. |
- Sekretaryova, Alina, et al.
(författare)
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Unsubstitutedand insoluble phenothiazine as an electron-transfer mediator in enzymaticelectrochemical biosensors
- 2013
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Ingår i: <em>Nano-scaled arrangements of proteins, aptamers, andother nucleic acid structures – and their potential applications</em> , COST Thematic Workshop, 8-9 October 2013, Helmholtz Zentrum fürUmweltforschung, Leipzig, Germany. - Leipzig : Helmholtz Zentrum für Umweltforschung. ; , s. O1-
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Konferensbidrag (refereegranskat)
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| 9. |
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| 10. |
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