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- Larsson, Lena, 1969, et al.
(författare)
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The Sp1 transcription factor binds to the G-allele of the -1087 IL-10 gene polymorphism and enhances transcriptional activation.
- 2009
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Ingår i: Genes and immunity. - : Springer Science and Business Media LLC. - 1466-4879 .- 1476-5470. ; 10, s. 280-284
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Tidskriftsartikel (refereegranskat)abstract
- The objectives of this study were to evaluate the influence of the -1087 single nucleotide polymorphism (SNP) on the gene expression of interleukin (IL)-10 and to identify transcription factors binding to this site in B cells. Using electrophoretic mobility-shift assays and nuclear extract from the DG75 B-cell line, we demonstrated that the Sp1 transcription factor bound to the -1087 G-allele of the IL-10 promoter and that the transcription factors PU.1 and Spi-B bound to both the G- and A-alleles. Transient transfections showed that lipopolysaccharide stimulation resulted in a 15-fold increase in promoter activity for the G-allele as compared to a 6-fold increase for the A-allele. Co-transfection with Sp1 expression vector in Sp1-deficient SL2 cells leading to Sp1 binding to the G-allele of the -1087 SNP resulted in increased IL-10 promoter activity. The results suggest a role for Sp1 transcription factor in the activation of IL-10 through the G-allele of the -1087 SNP in response to inflammatory signals.Genes and Immunity advance online publication, 9 October 2008; doi:10.1038/gene.2008.79.
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1648. |
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- Larsson, Lars, 1984, et al.
(författare)
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The transition matrix method for a 2D eddy current interaction problem
- 2012
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Ingår i: AIP Conference Proceedings. - : AIP. - 1551-7616 .- 0094-243X. ; 1430:31, s. 316-323
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Konferensbidrag (refereegranskat)abstract
- A 2D model of the eddy current interaction problem that consists of an inhomogeneityin a conductive half space is presented. The applied analytical method of solution is thetransition (T) matrix method. This involves use of the free space Green’s function to generate asystem of boundary integral relations. In this way, it is easy to identify the contributions to thetotal solution from each different scattering surface. The different parts are separated also inthe computation of the impedance. This leads to low cost simulations in terms of computationtime and qualify the method to be used to obtain probability of detection (POD) curves. TheT matrix method is a building block method and the possibility to extend the geometry withseveral inhomogeneities and extra layers will be discussed. The model is compared with a FiniteElement (FE) model and numerical examples for the case with a cylindrical inhomogeneity aregiven.
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