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Träfflista för sökning "WFRF:(Andersson Carina) srt2:(1995-1999)"

Sökning: WFRF:(Andersson Carina) > (1995-1999)

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  • Berg, Elisabeth, 1951-, et al. (författare)
  • Myggan
  • 1997
  • Bok (populärvet., debatt m.m.)
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  • Hellberg, Carina, et al. (författare)
  • Disruption of β2-integrin-cytoskeleton coupling abolishes the signaling capacity of these integrins on granulocytes
  • 1999
  • Ingår i: Biochemical and Biophysical Research Communications. - : Elsevier BV. - 0006-291X. ; 265:1, s. 164-169
  • Tidskriftsartikel (refereegranskat)abstract
    • Integrin dependent adhesion and dynamic modulations of the actin network are prerequisites for normal cell locomotion. To investigate whether the actin microfilamentous system does play a role in regulation of β2-integrin-induced signalling, we pretreated granulocytes with staurosporine, a well-known protein kinase inhibitor that has also been shown to disrupt the cytoskeleton of intact cells. Pretreatment with staurosporine completely inhibited the β2-integrin-induced Ca2+ signal and also its ability to trigger actin polymerisation. This inhibition was not related to phosphorylation of the CD18-chain of the β2-integrin, nor to inhibition of protein kinases. Instead, association of β2-integrins with the cortical cytoskeleton, which was observed in untreated cells, was abolished after exposure to staurosporine, indicating that β2-integrin signalling depends on integrin-cytoskeleton interaction. These results suggest not only that the actin network provides an adhesive link to the extracellular matrix and a driving force for the locomotory response, but also that it participates in regulation of β2-integrin signalling during granulocyte locomotion.
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