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  • Resultat 31-40 av 27345
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31.
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32.
  • Abdel-Rehim, Mohamed (författare)
  • Microextraction by packed sorbent (MEPS) : A tutorial
  • 2011
  • Ingår i: Analytica Chimica Acta. - : Elsevier BV. - 0003-2670 .- 1873-4324. ; 701:2, s. 119-128
  • Tidskriftsartikel (refereegranskat)abstract
    • This tutorial provides an overview on a new technique for sample preparation, microextraction by packed sorbent (MEPS). Not only the automation process by MEPS is the advantage but also the much smaller volumes of the samples, solvents and dead volumes in the system. Other significant advantages such as the speed and the simplicity of the sample preparation process are provided. In this tutorial the main concepts of MEPS will be elucidated. Different practical aspects in MEPS are addressed. The factors affecting MEPS performance will be discussed. The application of MEPS in clinical and pre-clinical studies for quantification of drugs and metabolites in blood, plasma and urine will be provided. A comparison between MEPS and other extraction techniques such as SPE, LLE, SPME and SBSE will be discussed. (C) 2011 Elsevier B.V. All rights reserved.
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36.
  • Abdel-Rehim, Mohamed, et al. (författare)
  • Microextraction in Packed Syringe Online with Liquid Chromatography-Tandem Mass Spectrometry : Molecularly imprinted polymer as packing material for MEPS in selective extraction of ropivacaine from plasma
  • 2006
  • Ingår i: Journal of Liquid Chromatography & Related Technologies. - : Informa UK Limited. - 1082-6076 .- 1520-572X. ; 29:12, s. 1725-1736
  • Tidskriftsartikel (refereegranskat)abstract
    • The excellent performance of a new sample preparation method, microextraction in packed syringe (MEPS), was recently illustrated by online LC‐MS and GS‐MS assays of local anaesthetics in plasma samples. In the method, approximately 1 mg of solid packing material was inserted into a syringe (100–250 µL) as a plug. Sample preparation took place on the packed bed. The new method was easy to use, fully automated, of low cost, and rapid in comparison with previously used methods. This paper presents the use of molecularly imprinted polymers (MIPs) as packing material for higher extraction selectivity. Development and validation of a method for MIP‐MEPS online with LC‐MS‐MS using ropivacaine in plasma as model compound were investigated. A bupivacaine imprinted polymer was used. The method was validated and the standard curves were evaluated by means of quadratic regression and weighted by inverse of the concentration: 1/x for the calibration range 2–2000 nM. The applied polymer could be used more than 100 times before the syringe was discarded. The extraction recovery was 60%. The results showed high correlation coefficients (R 2 >0.999) for all runs. The accuracy, given as a percentage deviation from the nominal concentration values, ranged from -6% to 3%. The precision, given as the relative standard deviation, at three different concentrations (QC samples) was consistently about 3% to 10%. The limit of quantification was 2 nM.
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38.
  • Abdel-Rehim, Mohamed (författare)
  • On-Line Whole Blood Analysis Using Microextraction by Packed Sorbent and LC-MS-MS
  • 2011
  • Ingår i: LC GC North America. - 1527-5949 .- 1939-1889. ; 29:7, s. 612-618
  • Tidskriftsartikel (refereegranskat)abstract
    • Microextraction by packed sorbent (MEPS) is a new technique for sample preparation that can be connected on-line with liquid chromatography (LC) or gas chromatography (GC) systems without any modifications. This article describes the use of MEPS in clinical and preclinical studies to quantify different drugs in whole blood samples. MEPS was used to determine cyclophosphamide in mouse blood from preclinical g studies using 20 mu L of blood samples. The interday accuracies and 0 precisions ranged from 107-109% and from 2.0-7.0%, respectively. The determination of four immunosuppressive drugs in human blood by MEPS and liquid chromatography-mass spectrometry (LC-MS) is described. The method showed a good selectivity and sensitivity. The calibration curves for everolimus, sirolimus, and tacrolimus ranged from 0.5 to 50 ng/mL and for cyclosporine from 3.0 to 1500 ng/mL. Intraday precisions for the studied immunosuppressive drugs were 2.0-11.7% and interday precision ranged from 5.1 to 13.7% (CV).
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40.
  • Abdollahi, Kamran, et al. (författare)
  • Improving Performance of On Demand Multicast Routing by deleting lost join query packet
  • 2010
  • Ingår i: Proceedings of The Sixth Advanced International Conference on Telecommunications - AICT 2010. - : IEEE conference proceedings. - 9780769540214 ; , s. 316-322
  • Konferensbidrag (refereegranskat)abstract
    • A Mobile ad hoc network is a collection of wireless nodes that dynamically organize themselves to form a network without the need for any fixed infrastructure or centralized administration. The network topology dynamically changes frequently in an unpredictable manner since nodes are free to move. Support for multicasting is essential in such environment as it is considered to be an efficient way to deliver information from source nodes to many client nodes. A problem with multicast routing algorithms is their efficiency as their forwarding structure determines the overall network resource consumption makes them significantly less efficient than unicast routing algorithms. In this research, we improve the performance of the popular ODMRP multicast routing protocol by restricting the domain of join query packets, which have been lost. This is achieved by augmenting the join query packets with minimum extra information (one field), which denotes the number of the visited node from previous forwarding group. Simulation results show, that our mechanisms significantly reduce the control traffic and thus the overall latency and power consumption in the network.
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